[1]李庆飞,王梦梦,张曼楠,等.不同储藏温度及时间对南瓜花粉生活力的影响[J].北方园艺,2020,44(10):116-121.[doi:10.11937/bfyy.20193630]
　LI Qingfei,WANG Mengmeng,ZHANG Mannan,et al.Effects of Different Storage Temperature and Time on Viability of Pumpkin Pollens[J].Northern Horticulture,2020,44(10):116-121.[doi:10.11937/bfyy.20193630]
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不同储藏温度及时间对南瓜花粉生活力的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年10 页码: 116-121 栏目: 出版日期: 2020-05-30
Title:
Effects of Different Storage Temperature and Time on Viability of Pumpkin Pollens
作者:
李庆飞1; 2; 王梦梦1; 张曼楠1; 王广印1; 2; 陈碧华1; 2; 李新峥1; 2
（1.河南科技学院 园艺园林学院，河南 新乡 453003；2.河南省园艺植物资源利用与种质创新工程研究中心，河南 新乡 453003）
Author(s):
LI Qingfei1; 2; WANG Mengmeng1; ZHANG Mannan1; WANG Guangyin1; 2; CHEN Bihua1; 2; LI Xinzheng1; 2
(1.College of Horticulture and Landscape,Henan Institute of Science and Technology,Xinxiang,Henan 453003;2.Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement,Xinxiang,Henan 453003)
关键词:
南瓜; 花粉生活力; 温度; 时间; 测定方法
Keywords:
pumpkin; pollen viability; temperature; time; assay methods
DOI:
10.11937/bfyy.20193630
文献标志码:
A
摘要:
以3个南瓜自交系的花粉为试材，采用醋酸洋红、I2-KI以及花粉体外萌发3种方法，对在室温储藏0、8、16、24、32 h及4 ℃储藏0、24、48、72、96 h的花粉活力进行测定，以期探究不同储藏温度及时间对南瓜花粉生活力的影响。结果表明：南瓜花粉活力在室温及4 ℃条件下随着时间的推移均表现为逐渐降低；室温储藏其活力下降较快，4 ℃储藏更适宜南瓜花粉的保存。在3种南瓜花粉活力测定方法中，醋酸洋红法测定值偏高，花粉体外萌发测定方法最可靠。另外，还改良了南瓜花粉体外萌发培养基配方，其萌发率远高于以往研究结果。该研究为解决生产上雌雄花花期不一致以及强雌系等雄花较少重要材料的授粉困难问题提供了参考依据。
Abstract:
To explore the effects of different storage temperature and time on pumpkin pollens,the pollens of three pumpkin inbred lines were used as materials，and the carmine acetate dyeing,I2-KI and germination in vitro methods were used to determine the viability of pollens,which treated for 0,8,16,24,32 hours at room temperature and treated for 0,24,48,72,96 hours at 4 ℃.The results showed that the pollen viability of pumpkin decreased gradually with time both at room temperature and 4 ℃,as well as the pollen viability decreased rapidly when stored at room temperature,thus 4 ℃ was more suitable for the preservation of pumpkin pollen.Among the three methods,the value of acetic acid magenta method was higher,and the method for pollen germination in vitro was the most reliable.In addition,this study also improved the medium formula of pollen in vitro germination,the germination rate was much higher than previous research.The results would provide a basis for solving the pollination problems of inconsistent male and female flowers and resource with few male flower such as subgynoecious lines.

参考文献/References:

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备注/Memo

第一作者简介：李庆飞（1988-），女，博士，讲师，现主要从事南瓜种质资源创新与利用等研究工作。E-mail:lqf_20131025@126.com.责任作者：李新峥（1965-），男，硕士，教授，现主要从事南瓜种质资源创新与利用等研究工作。E-mail:lxz22833@126.com.基金项目：河南省大宗蔬菜产业技术体系建设资助项目（S2010-03-G04）；河南省重点研发与推广专项资助项目（182102110049）；河南省大学生创新创业训练计划资助项目（S201910467016）。收稿日期：2019-11-29

更新日期/Last Update: 2020-07-25