[1]刘子记,刘维侠,牛玉,等.樱桃番茄Ph-3基因调控元件与同源基因系统进化分析[J].北方园艺,2020,44(06):31-37.[doi:10.11937/bfyy.20192798]
　LIU Ziji,LIU Weixia,NIU Yu,et al.Analysis of Ph-3 Gene Regulating Elements and Homologous Gene Evolution in Cherry Tomato[J].Northern Horticulture,2020,44(06):31-37.[doi:10.11937/bfyy.20192798]
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樱桃番茄Ph-3基因调控元件与同源基因系统进化分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年06 页码: 31-37 栏目: 出版日期: 2020-03-30
Title:
Analysis of Ph-3 Gene Regulating Elements and Homologous Gene Evolution in Cherry Tomato
作者:
刘子记; 刘维侠; 牛玉; 杨衍
（中国热带农业科学院 热带作物品种资源研究所，海南 儋州 571737）
Author(s):
LIU Ziji; LIU Weixia; NIU Yu; YANG Yan
(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737)
关键词:
樱桃番茄; 晚疫病; 顺式调控元件; 同源基因; 直系同源基因
Keywords:
cherry tomato; late blight; cis-regulatory elements; homologous genes; orthologous genes
DOI:
10.11937/bfyy.20192798
文献标志码:
A
摘要:
以樱桃番茄品种“京番黄星”为试材，采用PlantCARE在线分析和Gcorn系统进化分析方法，研究了Ph-3基因的表达调控元件及同源基因系统进化特征，以期为进一步解析Ph-3表达调控机制奠定基础。结果表明：Ph-3启动子除了含有核心元件外，还含有光响应元件、赤霉素响应元件、茉莉酸甲酯响应元件、厌氧诱导元件、昼夜节律调控响应元件等。Ph-3同源基因系统进化分析结果表明，当同源指数为0.70时，番茄、马铃薯、辣椒含有Ph-3的同源基因，且同源基因属于直系同源基因。另外，由同源指数分析结果发现，番茄与马铃薯的亲缘关系最近，其次为辣椒。
Abstract:
Taking cherry tomato variety ‘JingfanHuangxing’ as the test material,the expression regulatory elements of Ph-3 gene and the evolution characteristics of Ph-3 homologous genes had been studied by adopting PlantCARE and Gcorn phylogenetic analysis method,which could lay a foundation for further resolving the regulation mechanism of Ph-3 expression.The results showed that Ph-3 promoter not only contained the core elements,but also included light response element,gibberellin response element,methyl jasmonateresponse element,anaerobic induction elements,circadian response elements and so on.Ph-3 homologous gene phylogenetic analysis had showed that when the homology index was 0.70,tomato,potato and pepper contained Ph-3 homologous gene,and the homologous gene belonged to the orthologous gene.In addition,homology index analysis had showed that tomato had the closest relationship with potato,followed by pepper.

参考文献/References:

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备注/Memo

第一作者简介：刘子记（1982-），男，博士，副研究员，现主要从事蔬菜遗传育种等研究工作。E-mail:liuziji1982@163.com.责任作者：杨衍（1971-），男，博士，研究员，现主要从事蔬菜遗传育种等研究工作。E-mail：catasvegetable@163.com.基金项目：海南省科技资助项目（ZDYF2018035）。收稿日期：2019-09-19

更新日期/Last Update: 2020-05-05