HUO Jianfei,YAO Yurong,HAO Yongjuan,et al.Identification of Pepper Anthracnose and Screening of Fungicides Against It in Ninghe of Tianjin[J].Northern Horticulture,2020,44(03):1-7.[doi:10.11937/bfyy.20192149]
天津市宁河区辣椒炭疽病病原鉴定及防治药剂筛选
- Title:
- Identification of Pepper Anthracnose and Screening of Fungicides Against It in Ninghe of Tianjin
- Keywords:
- pepper; C.acutatum; C.truncatum; identification; screening of fungicides
- 文献标志码:
- A
- 摘要:
- 以从天津市宁河区采集的辣椒炭疽病病样为试材，通过形态学与对病菌核糖体DNA的ITS区分析相结合的方法对引起该病的病原进行鉴定，采用菌丝生长速率法测定了12种杀菌剂对病原菌的室内抑菌效果。结果表明：天津市宁河区辣椒主产区的辣椒炭疽病病原为尖孢炭疽菌和平头炭疽菌，二者的分离比率为0.711∶0.289。室内药剂筛选结果表明，咪鲜胺、咯菌腈、多菌灵和吡唑醚菌酯对尖孢炭疽菌菌丝抑制效果较好，其EC50值分别为0.022 8、0.123 2、0.361 2、0.716 8 μg·mL-1；咪鲜胺、咯菌腈和多菌灵对平头炭疽菌菌丝抑制效果较好，其EC50值分别为0.066 7、0.193 7、0.240 0 μg·mL-1。咪鲜胺、咯菌腈、多菌灵和吡唑醚菌酯可以作为防治辣椒炭疽病的候选药剂。
- Abstract:
- Pepper infected by Colletotrichum spp.in Ninghe,Tianjin was taken as experimental material,the pathogen by a combination of morphology and ribosomal DNA internal transcribed spacer regin(ITS) was identified,and twelve kinds of fungicides against the disease by plate method in the laboratary were screened.The results showed that the pathogen which infected pepper in Ninghe district was C.acutatum and C.truncatum,which segregation ratio was 0.711∶0.289,respectively.The results of fungicides screening in laboratory indicate,prochloraz,fludioxonil,carbendazim and pyradostrobin had better inhibitory effect on the growth of mycelium of C.acutatum,which EC50 values were 0.022 8,0.123 2,0.361 2,0.716 8 μg·mL-1,respectively.Prochloraz,fludioxonil and carbendazim had better inhibitory effect on the growth of mycelium of C.truncatum,which EC50 values were 0.066 7,0.193 7,0.240 0 μg·mL-1,respectively.Therefore,prochloraz,fludioxonil,carbendazim and pyraclostrobin could be used as chemicals to control the disease.
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备注/Memo
第一作者简介：霍建飞（1981），男，硕士，助理研究员，现主要从事蔬菜病害鉴定及防治技术等研究工作。Email:hjf2203@163.com.责任作者：王万立（1964），男，硕士，研究员，现主要从事蔬菜病害及杀菌剂应用技术等研究工作。Email:wlwang122@163.com.基金项目：天津市农业科学院青年科研人员创新研究与试验资助项目（2018017）；天津市农业科技成果转化与推广资助项目（201601280）；天津市蔬菜现代农业产业技术体系创新团队资助项目（ITTVRS2017010）。收稿日期：2019-09-16