LIU Kaige,GONG Fanrong,SONG Yunpeng,et al.Effects of Low Temperature and Weak Light on the Growth and Physiological and Biochemical Indexes of Sweet Pepper Seedlings and Their Relationship to Varieties Tolerance[J].Northern Horticulture,2020,44(03):8-14.[doi:10.11937/bfyy.20192253]
低温弱光对甜椒幼苗生长和生理生化指标的影响及其与品种耐性的关系
- Title:
- Effects of Low Temperature and Weak Light on the Growth and Physiological and Biochemical Indexes of Sweet Pepper Seedlings and Their Relationship to Varieties Tolerance
- Keywords:
- sweet pepper; low temperature and weak light stress; chlorophyll fluorescence parameters; greenhouse cultivation
- 文献标志码:
- A
- 摘要:
- 以甜椒品种‘P12101’（低温弱光敏感品种）和‘夏寒帝’（耐低温弱光品种）为试材，在光照培养箱中采用常规基质培养法，研究了低温弱光条件下甜椒生长指标、叶绿素含量、叶绿素荧光参数、相对电导率、丙二醛（MDA）含量以及超氧化物歧化酶（SOD）活性在不同处理天数下的变化，以期探讨甜椒耐低温弱光胁迫的生理机制和生理生化指标。结果表明：1）低温弱光胁迫下，甜椒幼苗的生长受到抑制，2个品种的株高、茎粗、地上鲜质量、地下鲜质量均呈下降趋势，‘P12101’的各生长指标下降幅度显著高于‘夏寒帝’；2个品种的叶绿素含量在低温弱光条件下也表现出下降趋势，其中‘P12101’显著低于‘夏寒帝’。2）2个甜椒品种在低温弱光处理下其叶绿色荧光参数Fo和Fv/Fm差异不显著，而Y（II）在处理10 d，Y（NPQ）和Y（NO）在处理15 d 2个品种表现出显著性差异。这表明虽然耐低温弱光品种在光能捕获吸收上同低温弱光敏感品种差异性不显著，但其在光能调节和利用上比后者更加有效。3）2个品种的MDA含量和相对电导率在低温弱光处理期间均呈逐渐升高趋势，并且‘P12101’显著高于‘夏寒帝’；4）低温弱光导致2个品种的SOD活性均呈先降后升趋势，处理15 d后，2个品种表现出显著性差异。对于甜椒品种耐低温弱光鉴定上，以处理15 d为宜，生长指标、叶绿素含量、电导率、MDA含量、叶绿素荧光参数Y（II）、Y（NPQ）和Y（NO）均可作为甜椒耐低温弱光鉴定指标，其中叶绿素荧光参数Y（II）、Y（NPQ）和Y（NO）较合适。
- Abstract:
- To elucidate the physiological mechanism of tolerance and the physiological and biochemical indexes of sweet pepper to low temperature and weak light stress ,the growth indicators,chlorophyll contents,chlorophyll fluorescence parameters,relative conductivities,malondialdehyde (MDA) content and the change of superoxide dismutase (SOD) activities in different treatment days were studied in two kinds of sweet pepper,‘P12101’ (a low temperature and weak light sensitive variety) and ‘Xiahandi’ (a low temperature and weak light resistant variety) by using the conventional medium culture method in the light incubator.The results showed that,1) the growth of sweet pepper seedings was inhibited under low temperature and weak light stress,the plant height,stem diameter,fresh shoot weight and fresh root weight of two varieties showed a downward trend.Compared to ‘Xiahandi’,the decline range of each growth index of ‘P12101’ was significantly higher.The content of chlorophyll of the two varieties also showed a downward trend under low temperature and low light,of which ‘P12101’ was significantly lower than that of ‘Xiahandi’.2) Chlorophyll fluorescence parameters Fo and Fv/Fm of two sweet pepper varieties had no significant difference under low temperature and week light stress.Y(II) showed remarkable difference after 10 days′ treatment,and Y(NPQ) and Y(NO) showed remarkable differences after 15 days′ treatment.This indicated that although there was no significant difference in optical energy capture and absorption between the low temperature and weak light resistant variety and the low temperature and weak light sensitive variety,the low temperature and weak light resistant variety was more effective in light energy regulation and utilization than the latter.3) MDA content and relative conductivities of the two varieties increased gradually during low temperature and week light treatment,and ‘P12101’ was significantly higher than ‘Xiahandi’.4) SOD activities of the two varieties decreased firstly and then increased due to low temperature and weak light treatment.After 15 days′ treatment,two varieties showed significant difference.The above results indicated that 15 days of treatment was the best time for identification of low temperature and weak light tolerance of sweet pepper varieties.Growth index,chlorophyll content,relative conductivity,MDA content,chlorophyll fluorescence parameters Y (II),Y (NPQ) and Y (NO) could be used as identification indexes of low temperature and weak light tolerance of sweet pepper,and among them,chlorophyll fluorescence parameters Y (II),Y (NPQ) and Y (NO) were more suitable.
参考文献/References:
[1]王洪涛,艾希珍,秦竞,等.几个甜椒砧木品种对低温弱光耐受性的差异[J].西北农业学报,2008,17(1):233-237.[2]钱芝龙,丁犁平,曹寿椿.低温胁迫对辣(甜)椒幼苗膜脂过氧化水平及保护酶活性的影响[J].园艺学报,1994,21(2):203-204.[3]沈齐,刘涛,徐刚,等.ALA对辣椒低温胁迫下伤害的缓解效应[J].江苏农业学报,2012,28(2):376-383.[4]马艳青,戴雄泽.低温胁迫对辣椒抗寒性相关生理指标的影响[J].湖南农业大学学报(自然科学版),2000,26(6):461-462.[5]柴文臣,马蓉丽,焦彦生,等.低温胁迫对不同辣椒品种生长及生理指标的影响[J].华北农学报,2010,25(2):168-171.[6]李晓,冯伟,曾晓春.叶绿素荧光分析技术及应用进展[J].西北植物学报,2006,26(10):2186-2196.[7]莫亿伟,郭振飞,谢江辉.温度胁迫对柱花草叶绿素荧光参数和光合速率的影响[J].草业学报,2011,20(1):96-101.[8]AMON D I.Copper enzymes in isolated chloroplasts polyphenol-oxidase in Beta vulgaris[J].Plant Physiology,1981,24(1):1-15.[9]HEATH R L,PACKER L.Photo peroxidation in isolated chloroplasts I.kinetics and stoichiometry of fatty acid peroxidation[J].Archives of Biochemistry and Biophysics,1968,125(1):189-198.[10]GIANNOPOLITIS N,RIES S K.Superoxide dismutase I.occurrence in higher plants[J].Plant Physiology,1977,59(2):309-314.[11]王平荣,张帆涛,高家旭,等.高等植物叶绿素生物合成的研究进展[J].西北植物学报,2009,29(3):629-636.[12]陈建明,俞晓平,程家安.叶绿素荧光动力学及其在植物抗逆生理研究中的应用[J].浙江农业学报,2006,18(1):51-55.[13]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.[14]KRAMER D M,JOHNSON G,KIIRATS O,et al.New fluorescence parameters for the determination of QA redox state and excitation energy fluxes[J].Photosynthesis Research 2004,79:209-218.[15]李衍素,高俊杰,陈民生,等.高温胁迫对豇豆幼苗叶片膜伤害与保护性物质的影响[J].山东农业大学学报(自然科学版),2007,38(3):378-382.[16]CHAOUI A,MAZHOUDI S,GHORBAL M H.Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean[J].Plant Science,1997,127(2):139-147.[17]郁继华,张国斌,冯致,等.低温弱光对辣椒幼苗抗氧化酶活性与质膜透性的影响[J].西北植物学报,2005,25(12):2478-2483.[18]侯伟,孙爱花,杨福孙,等.低温弱光对西瓜幼苗光合作用和抗氧化酶活性的影响[J].热带作物学报,2015,36(7):1232-1237.
相似文献/References:
[1]田聪聪,乜兰春,张哲,等.非草炭依赖型甜椒育苗基质筛选[J].北方园艺,2014,38(06):1.
TIAN Cong-cong,NIE Lan-chun,ZHANG Zhe,et al.Study on Screening of Substrate Without Peat for Sweet Pepper Seedling[J].Northern Horticulture,2014,38(03):1.
[2]蔡琳,汤志洪.不同棚膜覆盖对温室甜椒生长发育环境及产量的影响[J].北方园艺,2014,38(07):44.
CAI Lin,TANG Zhi-hong.Effect of Different Film Covering on Growth Environment and Yield of Peppers in Greenhouse[J].Northern Horticulture,2014,38(03):44.
[3]王洪霞,郭尚敬.TDZ对甜椒不定芽分化的影响[J].北方园艺,2012,36(03):104.
WANG Hong-xia,GUO Shang-jing.Effect of TDZ on Adventitious Bud Differentiation of Sweet Pepper[J].Northern Horticulture,2012,36(03):104.
[4]柴梦颖,焦镭.药用植物复配液在甜椒贮藏保鲜中的防腐效果[J].北方园艺,2012,36(07):171.
CHAI Meng-ying,JIAO Lei.Study on the Anti-corrosion Effect of Medicinal Plant Compound in Sweet Pepper Storage[J].Northern Horticulture,2012,36(03):171.
[5]贺泉兴,赵怀勇,贾改秀.甜椒新品种“平圆椒1号”的选育[J].北方园艺,2013,37(23):171.
HE Quan-xing,ZHAO Huai-yong,JIA Gai-xiu.Breeding of New Sweet Pepper Hybrid ‘Pingyuanjiao 1’[J].Northern Horticulture,2013,37(03):171.
[6]李小梅,赵洪彬,张景涛.甜椒新品种“哈椒8号”的选育[J].北方园艺,2015,39(12):150.[doi:10.11937/bfyy.201512040]
LI Xiaomei,ZHAO Hongbin,ZHANG Jingtao.Breeding of a New Hybrid Sweet Pepper Variety ‘Hajiao 8’[J].Northern Horticulture,2015,39(03):150.[doi:10.11937/bfyy.201512040]
[7]张溪荷,杨再强,陈艳秋,等.低温胁迫对东北四种设施作物叶片光合特性及抗氧化酶活性的影响[J].北方园艺,2015,39(10):15.[doi:10.11937/bfyy.201510004]
ZHANG Xi-he,YANG Zai-qiang,CHEN Yan-qiu,et al.Effect of Low Temperature Stress on the Leaves Photosynthesis and Autioxidant Enzyme Activities of Four Species Greenhouse Crops in Northeast of China[J].Northern Horticulture,2015,39(03):15.[doi:10.11937/bfyy.201510004]
[8]周 曼,李德明,蔡兴来,等.海南设施甜椒品比试验[J].北方园艺,2015,39(12):29.[doi:10.11937/bfyy.201512008]
ZHOU Man,LI De-ming,CAI Xing-lai,et al.Comparison of Sweet Pepper Cultivars for Protected Cultivation in Hainan[J].Northern Horticulture,2015,39(03):29.[doi:10.11937/bfyy.201512008]
[9]王 林 闯,贺 超 兴.追施不同氮肥对日光温室甜椒产量和品质的影响[J].北方园艺,2010,34(16):0.[doi:10.11937/bfyy.201016020]
W ANG Lin -chuang,HE Chao -xing.Effect of Different Nitrogenous Fertilizer T opdressing on the Yield and Quality of Sweet Pepper in Solar Greenhouse[J].Northern Horticulture,2010,34(03):0.[doi:10.11937/bfyy.201016020]
[10]颜津宁.凌源“奥黛丽”甜椒日光温室生产技术[J].北方园艺,2016,40(07):41.[doi:10.11937/bfyy.201607011]
[J].Northern Horticulture,2016,40(03):41.[doi:10.11937/bfyy.201607011]
备注/Memo
第一作者简介：刘凯歌（1988-），男，硕士，研究实习员，研究方向为甜椒生理与生物技术的研究与应用。E-mail:809509748@qq.com.责任作者：龚繁荣（1966-），男，本科，研究员，现主要从事甜椒和生菜育种及其生理等研究工作。E-mail:gongfr@126.com.基金项目：上海市科学技术委员会科研计划资助项目(13391901100)。收稿日期：2019-09-23