[1]崔蕴刚,张华敏,李延龙,等.韭菜灰霉病病原鉴定及其生物学特性[J].北方园艺,2020,44(04):14-19.[doi:10.11937/bfyy.20191800]
　CUI Yungang,ZHANG Huamin,LI Yanlong,et al.Identification and Biological Characterization of the Pathogen of Allium tuberosum Grey Mold[J].Northern Horticulture,2020,44(04):14-19.[doi:10.11937/bfyy.20191800]
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韭菜灰霉病病原鉴定及其生物学特性

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年04 页码: 14-19 栏目: 出版日期: 2020-02-28
Title:
Identification and Biological Characterization of the Pathogen of Allium tuberosum Grey Mold
作者:
崔蕴刚; 张华敏; 李延龙; 尹守恒; 张明; 马培芳
（平顶山市农业科学院，河南省韭菜工程技术研究中心，河南 平顶山 467001）
Author(s):
CUI Yungang; ZHANG Huamin; LI Yanlong; YIN Shouheng; ZHANG Ming; MA Peifang
(Pingdingshan Academy of Agricultural Sciences,Henan Chinese Chive Engineering Technology Research Center,Pingdingshan,Henan 467001)
关键词:
韭菜; 灰霉病; 病原菌; 葱鳞葡萄孢; 生物学特性
Keywords:
Allium tuberosum; gray mold; pathogen; Botrytis squamosa; biological characterization
DOI:
10.11937/bfyy.20191800
文献标志码:
A
摘要:
以斑点型韭菜灰霉病的病原菌为研究对象，采用柯赫氏法则、形态特征观察及SCAR标记检测等方法，对病原菌进行了鉴定，并研究了病原菌的生物学特性，以期为韭菜灰霉病的科学防治提供参考依据。结果表明：斑点型韭菜灰霉病的病原菌为葱鳞葡萄孢（Botrytis squamosa），其菌丝生长对环境条件的适应性很强，对光照不敏感，在葡萄糖、果糖、肌醇和D-半乳糖为碳源，pH 50~70的弱酸性培养基中，温度为15~25 ℃的冷凉条件下均能生长良好。葱鳞葡萄孢菌丝生长的最适碳源是葡萄糖，最适pH为60，最适温度为20 ℃，高温对菌丝生长有明显的抑制作用，30 ℃时，菌丝生长显著变慢，35 ℃时，菌丝停止生长。
Abstract:
The pathogen of spot type Allium tuberosum grey mould was used as the research object.The pathogen was identified according to Koch′s Rule,observation of morphological characteristics and SCAR marker detection,and the biological characteristics of pathogen were studied.The results showed that the pathogen of spot type A.tuberosum grey mould was Botrytis squamosa,and the mycelial growth of B.squamosa had strong adaptability to environmental conditions.The mycelia was insensitive to light,and could grow well in the weak acidic (pH 50-70) medium with glucose,fructose,inositol and D-galactose as carbon sources,under the cool conditions of 15-25 ℃.The optimum carbon source,pH,and temperature for mycelial growth of B.squamosa was glucose,60 and 20 ℃,respectively.At 30 ℃,the mycelial growth slowed down significantly,and at 35 ℃,the mycelial growth stop.

参考文献/References:

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备注/Memo

第一作者简介：崔蕴刚（1981-），男，硕士，助理研究员，研究方向为韭菜抗病和单倍体育种。E-mail:cui175@163.com.责任作者：张华敏（1982-），男，博士，助理研究员，研究方向为韭菜遗传育种与生物技术。E-mail:hmzhang111@126.com.基金项目：国家现代农业产业技术体系专项资金资助项目（CARS-24-A-11）；河南省科技攻关计划资助项目（152102110006）。收稿日期：2019-08-06

更新日期/Last Update: 2020-03-29