GAO Wa,ZHANG Dongmei,GAO Zhenjiang,et al.Identification of Pathogeny of Tomato Canker Disease and Screening of Resistance Varieties[J].Northern Horticulture,2019,43(23):20-26.[doi:10.11937/bfyy.20191132]
番茄溃疡病病原的鉴定和抗性品种的筛选
- Title:
- Identification of Pathogeny of Tomato Canker Disease and Screening of Resistance Varieties
- 文献标志码:
- A
- 摘要:
- 以番茄“CM966”病株为试验材料,采用PCR的方法和形态学鉴定法对包头市采集的疑似番茄病株进行鉴定,并构建系统发育树,以期确定其分类地位;以17个番茄品系和品种为试验材料,采用与番茄溃疡病相关的QTL位点中贡献率最高的Rcm5.1对17个番茄品系及品种进行特异性PCR扩增,同时进行田间接种试验,探究番茄溃疡病抗性种质资源。结果表明:采自4个不同番茄种植田的疑似溃疡病病株,均携带番茄溃疡病菌。经过BLAST比对,其中4个病原菌的分离物与GenBank中已报道的密执安棒形杆菌密执安亚种(Clavibacter michiganensis subsp.michiganensis,Cmm)的一致性达90%以上,其中1个病原菌分离物序列与其它菌株亲缘关系较远;对17个番茄品系及品种进行检测,均不携带QTL位点中Rcm5.1点的抗性基因,但是田间接种试验表明有3个品系‘山东荷兰’‘圣490’及‘紫晶’表现为高抗,病情指数分别为2%、4%、4%。“160”“CM966”‘T54’及“普罗旺斯”表现为高感,病情指数均大于50%。
- Abstract:
- Using tomato ‘CM966’ disease strain as test material,the suspected tomato disease strain collected by Baotou city was identified by PCR method and morphological identification method,and the phylogenetic tree was constructed to determine its taxonomic status;17 tomato lines and cultivars were tested with specific PCR amplification and using Rcm5.1 with the highest contribution rate in the QTL locus associated with tomato canker disease,and field inoculation test was conducted to explore tomato canker resistant species resources.The results showed that the suspected canker disease strains from four different tomato plantations all carried tomato canker.After BLAST comparison,the isolates of 4 pathogens were more than 90% identical to the reported Clavibacter michiganensis subsp.michiganensis (Cmm) in GenBank,including 1 pathogen.The isolate sequence was significantly different from other strains;17 tomato lines and cultivars were tested,and none of the resistance genes at the Rcm5.1 point in the QTL locus was carried.However,field vaccination trials showed that three strains ‘Shandong Holland’,‘Sheng 490’ and ‘Amethyst’ showed high resistance,with disease indices of 2%,4%,and 4%,respectively.‘160’‘CM966’‘T54’ and ‘Provence’ showed high susceptible,and the disease index was greater than 50%.
参考文献/References:
[1]罗来鑫,赵廷昌,李健强,等.番茄细菌性溃疡病研究进展[J].中国农业科学,2004,37(8):1144-1150.[2]GLEASON M L,GITAITIS R D,RICKER M D.Recent progress in understanding and controlling bacterial canker of tomato in eastern north America[J].Plant Disease,1993,77:1069-1076.[3]KAMASA J.Clavibacter michiganensis subsp.michiganensis[J].Eppo Bulletin,2013,43(1):46-67.[4]吴兴海,邵秀玲,厉艳,等.应用分子生物学方法快速检测番茄溃疡病菌的研究[J].江西农业学报,2006,18(2):5-7.[5]蔡昭雄,廖家榜,罗标,等.桂林市番茄溃疡病的发生及防治[J].中国蔬菜,2007(7):53-54.[6]何亮,陈群,曾忠铭,等.通过特异PCR扩增和16S rDNA序列分析检测动弯杆菌[J].微生物学报,2009,25(2):6-9.[7]于力,朱龙英,万延慧,等.利用多重PCR反应同时筛选番茄Tr-1和Cf-5基因[J].上海农业学报,2005,45(1):27-30.[8]张先练.植物数量性状的遗传与育种[M].呼和浩特:内蒙古大学出版社,1990.[9]FRANCIS D M,KABELKA E.Resistance to bacterial canker in tomato (Lycopersicon hirsutum LA407) and its progeny derived from crossed to L.esculeentum[J].Plant Disease,2001,85:1171-1175.[10]闵现华.番茄溃疡病菌的富集和快速分子生物学检测方法研究[D].兰州:兰州大学,2010.[11]PARK N Y,CHUNG C Y,MCLAREN A J,et al.Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis[J].FEMS Microbiology Letters,1995,125(2-3):225-229.[12]COAKER G L.Mapping,genetic effects,and epistatic interaction of two bacterial canker resistance QTLs from Lycopersicon hirsutum[J].Theoretical & Applied Genetics,2004,108(6):1047-1055.[13]王蕊,罗来鑫,李健强.不同来源番茄溃疡病菌致病力差异研究[J].植物保护,2010(1):73-77.[14]曹春梅.不同番茄品种对细菌性溃疡病的抗性测定及其抗病机理的初步研究[D].呼和浩特:内蒙古农业大学,2006.[15]高娃,张冬梅,刘丹,等.番茄溃疡病防治药剂的筛选[J].安徽农业科学,2018,46(20):137-140.[16]MANCEAU C.Assessment of genetic diversity among strains of Pseudomonas syringae by PCR-restriction fragment length polymorphism analysis of rRNA operons with special[J].Appl Environ Microbiol,1997,63(2):498-505.
相似文献/References:
[1]王 娜,卢宝慧,高 洁.树莓叶枯病的发生及病原菌的鉴定[J].北方园艺,2013,37(17):121.
WANG Na,LU Bao-hui,GAO Jie.Occurrence of Raspberry Leaf Blight and Its Pathogen Identification[J].Northern Horticulture,2013,37(23):121.
[2]胡彦江,张茹琴.烟台地区草莓根腐病病原鉴定及致病性测定[J].北方园艺,2012,36(10):141.
HU Yan-jiang ZHANG Ru-qin.Pathogenicity Test and Pathogen Identification of Strawberry Root Rot in Yantai Area[J].Northern Horticulture,2012,36(23):141.
[3]高国平,王 红,邓秋越,等.杨树叶缘枯病病原菌鉴定及生物学特性[J].北方园艺,2015,39(04):119.[doi:10.11937/bfyy.201504028]
GAO Guo-ping,WANG Hong,DENG Qiu-yue,et al.Study on Pathogen Identification and Biological Characteristics of Pathogenic Fungal of Poplar Leaf Margin Blight[J].Northern Horticulture,2015,39(23):119.[doi:10.11937/bfyy.201504028]
[4]毛芙蓉,李飞武,刘燕妮,等.番茄细菌性溃疡病菌的定性PCR检测方法[J].北方园艺,2015,39(05):128.[doi:10.11937/bfyy.201505037]
MAO Fu-rong,LI Fei-wu,LIU Yan-ni,et al.Qualitative PCR Detection Method for Clavibacter michiganensis subsp.Michiganensis[J].Northern Horticulture,2015,39(23):128.[doi:10.11937/bfyy.201505037]
[5]冯友仁,刘宝生,白鹏华.天津市新型月季叶枯病病原菌鉴定及生物学特性研究[J].北方园艺,2015,39(14):125.[doi:10.11937/bfyy.201514033]
FENG Youren,LIU Baosheng,BAI Penghua.Pathogen Identification and Biological Characteristics of Rose Leaf Blotch in Tianjin[J].Northern Horticulture,2015,39(23):125.[doi:10.11937/bfyy.201514033]
[6]阿里木?买买提,张静文,阿依夏木?亚库甫.新疆轮台县杏树侵染性流胶病病原菌的鉴定[J].北方园艺,2018,42(01):40.[doi:10.11937/bfyy.20172116]
AlimuMAIMAITI,ZHANG Jingwen,AyixiamuYAKUFU.Identification of Pathogen Causing Infectious Gummosis Disease ofApricot in Luntai of Xinjiang[J].Northern Horticulture,2018,42(23):40.[doi:10.11937/bfyy.20172116]
[7]尚晓静,罗妮星,张富美,等.树莓极细链格孢菌叶斑病病原菌鉴定及其生物学特性[J].北方园艺,2021,(16):24.[doi:10.11937/bfyy.20210163]
SHANG Xiaojing,LUO Nixing,ZHANG Fumei,et al.Identification of Alternaria tenuissima Pathogen of Raspberry Leaf Blotch and Its Biological Characteristics[J].Northern Horticulture,2021,(23):24.[doi:10.11937/bfyy.20210163]
[8]张琴,康明丽,赵茹,等.青皮核桃采后腐烂病病原菌分离与鉴定[J].北方园艺,2024,(4):74.[doi:10.11937/bfyy.20233560]
ZHANG Qin,KANG Mingli,ZHAO Ru,et al.Isolation and Identification of Pathogenic Bacteria Causing Postharvest Rot Disease in Green Walnuts[J].Northern Horticulture,2024,(23):74.[doi:10.11937/bfyy.20233560]
[9]王晓杰,侯文川,杨林林,等.箭叶淫羊藿灰霉病的病原菌鉴定及药剂筛选[J].北方园艺,2024,(4):89.[doi:10.11937/bfyy.20231968]
WANG Xiaojie,HOU Wenchuan,YANG Linlin,et al.Identification of Pathogen and Screening of Controlling Agents for Gray Mold Disease of Epimedium sagittatum (Sieb.et Zucc.) Maxim.[J].Northern Horticulture,2024,(23):89.[doi:10.11937/bfyy.20231968]
备注/Memo
第一作者简介:高娃(1987-),女,硕士,助理研究员,现主要从事植物保护等研究工作。E-mail:gaowabao@163.com.责任作者:张冬梅(1969-),女,本科,研究员,现主要从事植保农化等研究工作。E-mail:zhangdongmei69@126.com.基金项目:内蒙古自治区番茄工程中心资助项目(CX2017-51)。收稿日期:2019-05-03