LI Li,LIU Yiming,LI Yanyan,et al.Optimization of Ultrasound-assisted Extraction of Polyphenols From Boletus regius in Baoshan and Its Antioxidation[J].Northern Horticulture,2019,43(23):129-134.[doi:10.11937/bfyy.20190570]
超声波辅助保山桃红牛肝菌多酚提取工艺及其抗氧化活性
- Title:
- Optimization of Ultrasound-assisted Extraction of Polyphenols From Boletus regius in Baoshan and Its Antioxidation
- 文献标志码:
- A
- 摘要:
- 以云南保山桃红牛肝菌为试材，采用超声波辅助法提取多酚，使用正交实验法对提取工艺进行优化；通过测定桃红牛肝菌多酚对DPPH?、ABTS+?和?OH的清除能力评价其抗氧化活性。结果表明：最佳提取条件为乙醇体积分数50%，料液比1∶30 g?mL-1，超声时间50 min，超声功率100 W，在此工艺条件下桃红牛肝菌多酚得率为7.76 mg?g-1。桃红牛肝菌多酚呈现出较好的抗氧化活性，且与浓度成一定的正相关关系，在质量浓度为1.0 mg?mL-1时，对DPPH?、ABTS+?和?OH的清除率分别为42.3%、63.6%和66.5%。
- Abstract:
- Ultrasonic-assisted extraction of polyphenols,orthogonalization method was used to optimize the extraction process with Yunnan Baoshan Boletus regius;and the antioxidant activity was evaluated by the scavenging ability of DPPH?,ABTS+? and ?OH.The results showed that the optimal extraction processing parameters were ethanol concentration 50%,solid-liquid ratio 1∶30 g?mL-1,ultrasonic time 50 minutes and ultrasonic power 100 W.Boletus regius polyphenols showed good antioxidant activity and the antioxidant ability was correlated with the concentration,the clearance rates of DPPH?,ABTS+? and ?OH were respectively reached 42.3%,63.6% and 66.5% at the concentration of 1.0 mg?mL-1.
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备注/Memo
第一作者简介：李丽（1982-），女，硕士，副教授，研究方向为天然产物提取。E-mail：18660940@qq.com.基金项目：2018年国家级大学生创新创业训练计划资助项目（201810686003）；2018年保山学院校级课题面上资助项目（BYMS201821）。收稿日期：2019-05-03