DONG Shanshan,YANG Haiyan,WU Wenlong,et al.Physiological Characteristics Effects of Mn Stress on Rabbiteye Blueberry ‘Gardenblae’[J].Northern Horticulture,2019,43(12):34-38.[doi:10.11937/bfyy.20183603]
兔眼蓝莓品种‘Gardenblue’对锰胁迫的响应机制
- Title:
- Physiological Characteristics Effects of Mn Stress on Rabbiteye Blueberry ‘Gardenblae’
- Keywords:
- blueberry; Mn stress; physiological resistance
- 文献标志码:
- A
- 摘要:
- 以兔眼蓝莓‘Gardenblue’为试材，采用盆栽试验的方法，研究了不同浓度（0、1.0、2.5、5.0、10.0 mmol?L-1）Mn2+胁迫下蓝莓叶片生理和结构的变化。Mn2+胁迫下，叶片中叶绿素（Chl）、类胡萝卜素（Car）和可溶性蛋白质（SP）含量先升后降；过氧化氢（H2O2）、脯氨酸（Pro）含量呈下降趋势，丙二醛（MDA）含量10 mmol?L-1 Mn2+处理时有所下降；水杨酸（SA）和乙烯前体（ACC）含量呈上升趋势，脱落酸（ABA）含量在10 mmol?L-1 Mn2+处理之前较稳定；叶片气孔及表皮细胞无显著变化。综上所述，‘Gardenblue’对过量的锰具有一定的耐受性，其中1.0～5.0 mmol?L-1 Mn2+处理浓度适合其生长。
- Abstract:
- The physiological and structural changes of blueberry leaves under different concentrations of Mn2+ were studied by pot experiment with rabbiteye blueberry ‘Gardenblue’ as material.Under Mn2+ stress，the contents of chlorophyll (Chl)，carotenoid (Car) and soluble protein (SP) increased first and then decreased;hydrogen peroxide (H2O2) and proline (Pro) contents decreased，Malondialdehyde (MDA) content decreased under 10 mmol?L-1 Mn2+ treatment，salicylic acid (SA) and ethylene precursor (ACC) content increased and abscisic acid (ABA) content was stable before 10 mmol?L-1 Mn2+ treatment;leaf stomata and epidermal cells did not change significantly.In summary，‘Gardenblue’ had a certain tolerance to excess manganese，and 1.0-5.0 mmol?L-1 Mn2+ treatment concentration was suitable for its growth.
参考文献/References:
[1]文珂,郭晓玉,谭娜娜,等.锰胁迫对野大豆种子萌发及幼苗生理生化特征的影响[J].种子,2018，37(3):40-45.[2]牟东岭.葡萄(Vitisvinifera×V.labrusca)和刺梨(Rosaroxburghii)对锰胁迫的生理响应差异[D].贵阳:贵州大学，2010.[3]孙海悦,李亚东.世界蓝莓育种概述[J].东北农业大学学报，2014，45(9):116-112.[4]杨喜平,张胜利,王琚钢,等.我国蓝莓研究[J].内蒙古林业科技，2012，38(3):55-58.[5]HOAGLAND D R,ARNON D I.The water-culture method for growing plants without soil[J].Circular California Agricultural Experiment Station，1950，347:1-32.[6]ARNON D I.Copper enzymes in isolated chloroplasts polyphenol oxidase in Beta vulgaris[J].Plant Physiol，1949，24:1-5.[7]HEATH R L,PARKER L.Photoperoxidation in isolated chloroplasts I.Kinetics and stoichiometry of fatty acid peroxidation[J].Arch Biochem Biophys，1968，125(1):189-193.[8]BATES L E,WALDREN R P,TEARE I D.Rapid determination of free proline for water stress studies[J].Plant Soil，1973，39(1):205-207.[9]吴旭红,张超.锰胁迫对甜菜幼苗光合色素含量及碳氮代谢相关酶活性的影响[J].中国糖料，2011(4):42-45,48.[10]LIANGW B,XUE S G,SHEN J H,et al.Effects of manganese stress on photosythesis and chlorophyll fluorescence parameters of Phytolacca americana[J].Acta Ecologica Sinica，2010，30(3):619-625.[11]全先庆,张渝洁,单雷,等.脯氨酸在植物生长和非生物胁迫耐受中的作用[J].生物技术通讯，2007，18(1):159-162.[12]JOHN R,AHMAD P,GADGIL K,et al.Effect of cadmium and lead on growth，biochemical parameters and uptake in Lemna polyrrhiza L.[J].Plant Soil Environ，2008，54(6):262-270.[13]YU Y W，HUANG R F.Ethylene and plant resistance to adversity[J].J Agr Sci Tech-Irna，2013，15(2):70-75.[14]JING C.Effects of salicylic acid on resisting adversity of plants[J].Anhui Agri Sci Bull，2007(9):16.[15]赵艳侠,亓桂梅,王咏梅.水杨酸对葡萄抗逆性调控的研究进展[J].山东农业科学，2017，49(11):146-150.[16]LENG P,YUAN B,GUO Y.The role of abscisic acid in fruit ripening and responses to abiotic stress[J].J Exp Bot，2014，65(16):4577-4588.
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备注/Memo
第一作者简介：董珊珊（1992-），女，硕士研究生，研究方向为黑莓、蓝莓等小浆果生理。E-mail:15305183284@163.com.责任作者：李维林（1966-），男，博士，研究员，现主要从事黑莓、蓝莓等小浆果栽培及生理生化和分子等研究工作。E-mail:wlli@njfu.edu.cn.基金项目：江苏省农业科技自主创新资金资助项目（CX(17)2011）；江苏省农业三新工程资助项目（SXGC[2017]309）。收稿日期：2018-12-03