GUO Xue,LI Xingguo,SUN Qiaoye,et al.Optimization of Pressurised Water Extraction of Polysaccharides From Vaccinium uliginosum L. Using Response Surface Methology[J].Northern Horticulture,2019,43(05):116-122.[doi:10.11937/bfyy.20182804]
响应面试验优化加压水提法提取笃斯越桔多糖的工艺研究
- Title:
- Optimization of Pressurised Water Extraction of Polysaccharides From Vaccinium uliginosum L. Using Response Surface Methology
- Keywords:
- Vaccinium uliginosum L.; pressurised water extraction; polysaccharides; response surface methodology; extraction
- 文献标志码:
- A
- 摘要:
- 以笃斯越桔(Vaccinium uliginosum L.)为试材，在单因素试验基础上，考察提取时间、提取压力、提取温度对笃斯越桔粗多糖得率的影响；利用Box-Behnken中心组合试验建立数学模型，采用响应面优化加压水提法提取笃斯越桔多糖的工艺,以期为深入研究笃斯越桔果实多糖提供参考依据。结果表明：最优提取工艺为提取时间23 min、提取压力1.2 MPa，提取温度81 ℃，笃斯越桔粗多糖得率达（7.48±0.11）%，与预测值的相对误差为0.09%，说明响应面法优化笃斯越桔果实中提取多糖的工艺可行。
- Abstract:
- Taking Vaccinium uliginosum L.as test material，pressurised water extraction (PWE) of polysaccharides from Vaccinium uliginosum L.fruits was investigated using a response surface methodology (RSM).The effects of extraction time，extraction pressures and extraction temperature on the yield of polysaccharides were examined by using single factor experiments.A mathematical model was established using Box-Behnken center combination test，and response surface analysis was performed to determine the optimal extraction conditions,inorder to procide reference for the study of polysaccharides of Vaccinium uliginosum L. fruit.The results showed that the optimum conditions were extraction time 23 minutes，extraction pressures 1.2 MPa，extraction temperature 81 ℃，extraction rate of polysaccharides was 7.48%，with relative error of 0.09%，which revealed response surface methodology was feasible to optimize the pressurised water extraction of polysaccharides from Vaccinium uliginosum L..
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备注/Memo
第一作者简介：郭雪（1993-），女，硕士研究生，研究方向为果树采后生理。E-mail:2459707808@qq.com.责任作者：于泽源（1961-），男，博士，教授，现主要从事果树采后生理等研究工作。E-mail:yzy@neau.edu.cn.收稿日期：2018-10-17