FAN Lei,GAO Zhiying.Cloning and Expression Analysis of SlWRKY44 Gene in Tomato[J].Northern Horticulture,2018,42(22):6-10.[doi:10.11937/bfyy.20181435]
番茄SlWRKY44基因的克隆及表达
- Title:
- Cloning and Expression Analysis of SlWRKY44 Gene in Tomato
- Keywords:
- Solanum lycopersicum; SlWRKY44; cloning; expression analysis
- 文献标志码:
- A
- 摘要:
- 植物WRKY转录因子基因在植物逆境应答中具有重要的作用。以盐胁迫的番茄为试材，采用RTPCR和荧光定量PCR方法，研究了番茄SlWRKY44基因序列和表达模式，以期为研究该基因在盐胁迫应答中的功能提供参考依据。结果表明：SlWRKY44基因编码区长984 bp，编码327个氨基酸，预测分子量约为36.72 kDa，等电点为5.79。推测的氨基酸序列中含有1个高度保守的WRKY结构域。亚细胞定位预测SlWRKY44蛋白定位于细胞核。进化分析发现，SlWRKY44和拟南芥AtWRKY46、AtWRKY53和AtWRKY54为一个分支。组织特异性表达模式分析表明，在检测的所有组织中SlWRKY44均有表达，在根中表达量最高，在茎中表达量最低。SlWRKY44基因的表达受高盐诱导，说明番茄SlWRKY44基因可能参与番茄对高盐胁迫的应答。该研究为进一步分析该基因在盐胁迫应答中的功能提供了参考依据。
- Abstract:
- WRKYdomain proteins play important roles in plant stress responses.The salttreated tomato was used as material,the sequence and expression pattern of tomato SlWRKY44 gene were explored by using RTPCR and realtime PCR technology,in order to provide a reference for studying the function of this gene in salt stress response.The results showed that the full length SlWRKY44 CDS was 984 bp.This CDS sequence encoded a polypepide of 327 amino acid residues with a predicted molecular mass of 36.72 kDa and a basic isoelectric point of 5.79,and a highly conserved WRKY domain in the encoded putative protein.Protein subcellular localization predicted indicated that SlWRKY44 was localized in the nucleus.Phylogenetic analysis indicated that SlWRKY44 clustered with AtWRKY46,AtWRKY53 and AtWRKY54.Tissuespecific expression profiles showed that SlWRKY44 was nearly expressed in all detected tissues,and its expression level was relatively high at root and low at stem.Quantitative RTPCR analysis showed that SlWRKY44 was induced by salt stress.The expression patterns of SlWRKY44 under salt stress suggested that this gene might be involved in the regulations of tomato to salt stress.
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备注/Memo
第一作者简介：樊蕾（1983），女，山西运城人，硕士，讲师，现主要从事蔬菜育种等研究工作。Email：2162434367@qq.com.基金项目：山西运城农业职业技术学院资助项目(2016AB0005，2017AB0005)。收稿日期：2018-06-30