HUANG Chao,YAO Xue,WANG Xuezheng,et al.Construction of Comprehensive Evaluation System for Cold Tolerance in Melon[J].Northern Horticulture,2018,42(22):43-51.[doi:10.11937/bfyy.20181029]
甜瓜耐冷性综合评价体系的构建
- Title:
- Construction of Comprehensive Evaluation System for Cold Tolerance in Melon
- Keywords:
- melon（Cucumis melo L.）; cold tolerance; principal components analysis; regression analysis
- 文献标志码:
- A
- 摘要:
- 以14份遗传背景差异明显的甜瓜品种为试材，采用低温处理的方法，研究了芽期16 ℃和18 ℃低温胁迫下种子相对发芽率、相对发芽势、相对发芽指数、相对胚根长度、相对种芽质量及相对活力指数，苗期4、7 ℃低温胁迫下处理8、16、24 h后测定相对电导率、丙二醛含量、脯氨酸含量及过氧化物酶活性，并运用多种分析方式对甜瓜耐寒性鉴定指标进行筛选和评价，以期为耐低温甜瓜新品种的选育和大规模甜瓜品种耐冷性评价提供参考依据。结果表明：18 ℃下芽期各项指标在甜瓜品种间差异更加显著，为最适芽期胁迫温度；7 ℃处理16 h下苗期各项指标差异范围更为明显，为最适苗期胁迫条件；通过主成分分析将10个单项指标转换为3个相互独立的综合指标；通过隶属函数和聚类分析将14份甜瓜品种分为4类：强耐寒型、中度耐寒类型、不耐寒类型和寒敏感类型；通过逐步回归建立了甜瓜耐冷性的评价数学模型Y=0.404 9＋0.400 4X.2＋0.177 5X.5-0.029 1X.8-0.004 2X.9（R2=0.999 6），估计精度大于96.75%，并筛选出4个甜瓜耐冷性鉴定指标，分别为相对发芽势、相对种芽质量、丙二醛含量和脯氨酸含量。研究结果可为耐低温甜瓜新品种的选育奠定基础。
- Abstract:
- The physiological and biochemical values were measured in fourteen melon cultivars after low temperature stress,including relative germination rate,relative germination potential,relative germination index,relative radicle length,relative shoots weight and relative vigor index during germination stage under 16 ℃ and 18 ℃ and relative electric conductivity,malondialdehyde content,proline content and peroxidase activity in leaves of melon seedlings under low temperature stress (4 ℃,7 ℃ for 8 hours,16 hours,24 hours，respectively).Identification methods and data of germination and seedling stages were evaluated under different low temperature stress conditions by multiple analytical methods,in order to provide a reference for the melon breeding of low temperature tolerance and the evaluation of cold tolerance of largescale melon varieties.The results showed that the suitable intensity was 18 ℃ at bud stage and 7 ℃ with treated 16 hours at seedling stage.Ten single indexes could be converted three independent comprehensive components by principal components analysis,14 melon cultivars were divided into four chillingtolerance types by cluster analysis.The mathematical evaluation model for melon cold tolerance was established,Y=0.404 9+0.400 4X.2+0.177 5X.5-0.029 1X.8-0.004 2X.9(R2=0.999 6),and the evaluation accuracy of the equation was higher than 96.75%,and four indexes closely related to the chilling tolerance were determined,which were relative germination percentage,relative shoots weight,malondialdehyde content and proline content.It would provide the basis for selection of cold tolerance melon.
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备注/Memo
第一作者简介：黄超（1992），女，硕士研究生，研究方向为蔬菜遗传育种。Email：858292575@qq.com.责任作者：刘宏宇（1967），男，硕士，副研究员，硕士生导师，研究方向为蔬菜遗传育种。Email：hyliu@neau.edu.cn.基金项目：国家自然科学基金资助项目(31372088)；东北农业大学“学术骨干”资助项目（15X025）。收稿日期：2018-06-06