CHANG Qingquan,WANG Hailian,BAI Xiaoxian,et al.Extraction of Polysaccharide and Antioxidant Activity From Actinidia arguta Stems and Leaves in Changbai Mountain[J].Northern Horticulture,2018,42(19):136-144.[doi:10.11937/bfyy.20180316]
长白山产软枣猕猴桃茎叶多糖提取及抗氧化活性
- Title:
- Extraction of Polysaccharide and Antioxidant Activity From Actinidia arguta Stems and Leaves in Changbai Mountain
- Keywords:
- Actinidia arguta stems and leaves; polysaccharide; ultrasonic extraction; microwave-assisted extraction; response surface methodology
- 文献标志码:
- A
- 摘要:
- 以长白山产软枣猕猴桃茎、叶为原料，以茎叶多糖得率为指标，利用超声提取叶多糖微波提取茎多糖，通过单因素试验和响应面试验优化最佳提取方法，得到茎、叶多糖最优提取条件。结果表明：茎多糖提取工艺为微波时间40 min、微波功率315 W、液料比59 mL·g-1，茎多糖得率为2.85%；叶多糖超声提取工艺为超声时间40 min、液料比为40 mL·g-1、超声提取功率为900 W，在此条件下叶多糖得率为4.50%；提取得到的茎、叶多糖均具有清除DPPH自由基的能力，可以作为一种天然的抗氧化剂进一步开发利用。
- Abstract:
- The stems and leaves of Actinidia arguta were used as test materials,using the polysaccharide yields as index,the ultrasonic assisted extraction method was chosen for extraction leaves polysaccharides,and the microwave-assisted extraction method was chosen for extraction stems method.The optimum extraction methods of polysaccharide were determined on the base of single test and response surface methodology.The results showed that the optimum technology of leaves was that ultrasonic time was 40 minutes,liquid-solid ratio was 40 mL·g-1 and the ultrasonic power was 900 W.Under the condition the yield of leaves polysaccharide was 4.50%.The optimum technology of stems was that the microwave time was 40 minutes,the microwave power was 315 W,the liquid-solid ratio was 59 mL·g-1,under the condition the yield of stems polysaccharide was 2.85%.The polysaccharide extraction from the stems and leaves could scavenge DPPH,and the polysaccharides could be used as natural antioxidant,to be further exploited.
相似文献/References:
[1]李海平,何玥,闫军,等.响应面法优化超声提取地皮菜多糖工艺研究[J].北方园艺,2013,37(04):138.
[2]谭启明,付瑞敏,李景原,等.响应面法优化微波辅助提取芦荟凝胶多糖工艺[J].北方园艺,2014,38(13):133.
TAN Qi-ming,FU Rui-min,LI Jing-yuan,et al.Optimization of Microwave-assisted Extraction Process of Aloe barbadensis Miller Polysaccharies by Response Surface Method[J].Northern Horticulture,2014,38(19):133.
[3]徐 雷,刘常丽,张 群,等.不同初加工方法对茯苓多糖和三萜类成分的影响[J].北方园艺,2014,38(10):148.
XU Lei,LIU Chang-li,ZHANG Qun,et al.Effect of Different Processing Methods on Polysaccharides and Triterpenes of?Poria [J].Northern Horticulture,2014,38(19):148.
[4]罗 琴,林惠昆,管春平,等.正交实验法优化提取二型鳞毛蕨多糖工艺的研究[J].北方园艺,2013,37(22):146.
LUO Qin,LIN Hui-kun,GUAN Chun-ping,et al.Study on the Optimization of Extraction Technology of Polysaccharide from Dryopteris cochleata Using Orthogonal Experiment[J].Northern Horticulture,2013,37(19):146.
[5]陈玉琴,南海娟,刘坤峰.蕨菜多糖提取及抗氧化特性[J].北方园艺,2014,38(14):128.
CHEN Yu-qin,NAN Hai-juan,LIU Kun-feng.Extraction Technology and Antioxidant Acitvity of Polysaccharide From Pteridium revolutum [J].Northern Horticulture,2014,38(19):128.
[6]魏楠楠,姚 睿,李 阳,等.几种因素对反应器铁皮石斛原球茎生长及有效物质的影响[J].北方园艺,2014,38(14):156.
WEI Nan-nan,YAO Rui,LI Yang,et al.Effect of Several Factors on Accumulation of Biomass and Bioactive Compounds during Bioreactor Culture of Dendrobium candidum PLB[J].Northern Horticulture,2014,38(19):156.
[7]龚庆芳,赵 健,何金祥,等.遮荫对一年生铁皮石斛生长特性、光合特性及多糖含量的影响[J].北方园艺,2014,38(01):148.
GONG Qing-fang,ZHAO Jian,HE Jin-xiang,et al.Effects of Shade Treatment on the Growth and Photosynthetic Characteristics and Polysaccharides Content of Annual Dendrobium officinale [J].Northern Horticulture,2014,38(19):148.
[8]韩凤波,奚广生.不同采收期对轮叶党参有效成分含量的影响[J].北方园艺,2014,38(01):154.
HAN Feng-bo,XI Guang-sheng.Effect of Different Harvest Time on the Effective Component Content of Codonopsis lanceolata [J].Northern Horticulture,2014,38(19):154.
[9]姜少娟.毛竹叶多糖超声提取工艺研究[J].北方园艺,2013,37(13):166.
JIANG Shao-juan.Research on Ultrasonic Wave Extraction of Polysaccharides from Phyllostachys pubescens Leaves[J].Northern Horticulture,2013,37(19):166.
[10]胡月芳.淮山多糖微波法提取及清除羟基自由基的研究[J].北方园艺,2014,38(02):135.
HU Yue-fang.Study on the Microwave Extraction and Hydroxy Radical Scavenging of Yam Polysaccharide[J].Northern Horticulture,2014,38(19):135.
备注/Memo
第一作者简介：常清泉（1995-），女，硕士研究生，研究方向为天然有机化学。E-mail:Diana65154@163.com.责任作者：陆娟（1982-），女，博士，副教授，研究方向为天然有机化学。E-mail:smilecui0513@163.com.基金项目：长春师范大学2017年度研究生教育创新计划资助项目（cxcxy2017031）;2017年长春师范大学大学生创新创业训练计划资助项目（国家级，201710205009）。收稿日期：2018-03-19