ZHANGShuihua,LIBenhong.Microwave-assistedExtractionandAntioxidantActivityof PolysaccharidesFromTricholomaflavovirensLundell[J].Northern Horticulture,2018,42(20):120-124.[doi:10.11937/bfyy.20180052]
微波辅助提取油黄口蘑多糖工艺及其体外抗氧化性研究
- Title:
- Microwave-assistedExtractionandAntioxidantActivityof PolysaccharidesFromTricholomaflavovirensLundell
- Keywords:
- Tricholomaflavovirens; polysaccharides; microwave-assisted; DPPH·; ABTS+·
- 文献标志码:
- A
- 摘要:
- 以云南野生油黄口蘑为试材，研究了液料比、微波功率、微波时间、提取次数对多糖提取率的影响，在单因素试验基础上，采用正交实验优化了工艺条件，对最佳工艺条件下提取产物进行了DPPH·、ABTS+·清除研究。结果表明：对多糖提取率影响最大的是微波时间，其次是液料比，提取次数影响最小；优化工艺条件为液料比15∶1mL·g-1，微波功率320W，微波时间3min，提取3次，在此条件下多糖提取率达4.11%。抗氧化性研究表明，多糖具有良好的抗氧化能力。在一定浓度范围内，清除能力与浓度呈正相关，当多糖浓度分别为3.5、2.5g·L-1时对DPPH·及ABTS+·的清除率分别达到88%和90%。
- Abstract:
- TakingTricholomaflavovirensastherawmaterial,theeffectsofextractionconditionssuchasliquid-solid,microwavetime,microwavepowerandextractiontimeswereinvestigatedbyone-factortestsandorthogonaltest.AndtheextractionpolysaccharideswereanalyzedbyDPPH·andABTS+·test.Theresultsshowedthattheextractiontimeaffectedtheyieldmarkably,secondlywassolid-liquidratio,andtheextractiontimeshadtheleastinfluence；theoptimumconditionsweredeterminedasfollows：solid-liquidratio15∶1mL·g-1,microwavepowerof320W,microwavetimeof3minutesandextractiontimesof3times.Underthecondition,theyieldwasupto4.11%.Theantioxidationexperimentsindicatedthatthepolysaccharideshadgoodoxidationresistantability.Andthescavengingabilityrelatedwiththeconcentrations,attheconcentrationof3.5g·L-1and2.5g·L-1，theDPPH·andABTS+·scavengingratewere88%and90%.
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备注/Memo
第一作者简介：张水花（1980-），女，硕士，副教授，研究方向为天然产物化学及保健食品。E-mail:z_shh@163.com.基金项目：云南省科技厅研究基金资助项目（2016FD081）。收稿日期：2018-03-28