[1]唐尧,张微,尹艳莉,等.拟南芥[STBX]AtOFP8[STBZ]的生物信息学分析及表达分析[J].北方园艺,2018,42(18):35-41.[doi:10.11937/bfyy.20174346]
　TANG Yao,ZHANG Wei,YIN Yanli,et al.Bioinformatics Analysis and Expression Analysis of AtOFP8 in Arabidopsis thaliana[J].Northern Horticulture,2018,42(18):35-41.[doi:10.11937/bfyy.20174346]
点击复制

拟南芥[STBX]AtOFP8[STBZ]的生物信息学分析及表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年18 页码: 35-41 栏目: 出版日期: 2018-09-25
Title:
Bioinformatics Analysis and Expression Analysis of AtOFP8 in Arabidopsis thaliana
作者:
唐尧1; 张微1; 2; 尹艳莉1; 冯鹏1; 陈宇峰1; 常缨1
（1.东北农业大学 生命科学学院，黑龙江 哈尔滨 150030；2.黑龙江省农业科学院 克山分院，黑龙江 克山 160000）
Author(s):
TANG Yao1; ZHANG Wei1; 2; YIN Yanli1; FENG Peng1; CHEN Yufeng1; CHANG Ying1
(1.College of Life Sciences,Northeast Agricultural University,Harbin,Heilongjiang 150030;2.Keshan Branch,Heilongjiang Academy of Agricultural Sciences,Keshan,Heilongjiang 160000)
关键词:
拟南芥; 转录因子; AtOFP8; 生物信息学
Keywords:
Arabidopsis thaliana; transcription factor; AtOFP8; bioinformatics
DOI:
10.11937/bfyy.20174346
文献标志码:
A
摘要:
以Columbia-0拟南芥为试材，采用生物信息学方法对拟南芥转录因子[STBX]AtOFP8[STBZ]进行生物信息学分析，应用荧光定量PCR技术对[STBX]AtOFP8[STBZ]基因进行组织表达分析和逆境表达分析，为进一步研究其它AtOFP的功能奠定基础。结果表明：[STBX]AtOFP8[STBZ]基因全长954 bp，编码蛋白由221个氨基酸组成，是一个分子量为25.466 kDa的不稳定蛋白，该蛋白无跨膜区和信号肽，定位在细胞核中，亲水能力较强；[STBX]AtOFP8[STBZ]基因在根和花中表达较高，在高温干旱等环境下转录水平明显升高。
Abstract:
The Columbia-0 Arabidopsis thaliana was used as material.Bioinformatics analysis of Arabidopsis transcription factor AtOFP8 was carried out by using bioinformatics methods and [STBX]AtOFP8[STBZ] gene expression analysis was used by fluorescence quantitative PCR technology.Both of them laid the foundation for further research on the function of other AtOFPs.The results showed that the full-length of [STBX]AtOFP8[STBZ] gene was 954 bp,AtOFP8 protein was composed of 221 amino acids,its molecular weight was 25.466 kDa,it had no transmembrane domain and signal peptide,it was located in the nucleus and it had strong hydrophilicity.In addition,[STBX]AtOFP8[STBZ] gene was highly expressed in roots and flowers,and its transcriptional level was obviously elevated in the environment of high temperature and drought.

相似文献/References:

[1]侯丽霞,王文杰,刘新.葡萄VvWRKY13转录因子的生物信息学分析[J].北方园艺,2013,37(13):117.
　HOU Li-xia,WANG Wen-jie,LIU Xin.Bioinformatics Analysis of VvWRKY13 Transcription Factor in Vitis vinifera L.[J].Northern Horticulture,2013,37(18):117.
[2]陈昱,官丽莉,杨晶,等.植物金属硫蛋白的研究进展[J].北方园艺,2014,38(07):185.
　CHEN Yu,GUAN Li-li,YANG Jing,et al.Research Progress on Plant Metallothionein[J].Northern Horticulture,2014,38(18):185.
[3]李学鑫,白志川,岳欢,等.甜橙WRKY转录因子序列的生物信息学分析[J].北方园艺,2013,37(01):119.
　LI Xue-xin,BAI Zhi-chuan,YUE Huan,et al.Bioinformatics Analysis of Orange WRKY Transcription Factor Sequence[J].Northern Horticulture,2013,37(18):119.
[4]尹跃,曹有龙,陈晓静,等.枸杞MYB103转录因子的生物信息学分析[J].北方园艺,2012,36(14):118.
　YIN Yue,CAO You-long,CHEN Xiao-jing,et al.Bioinformatics Analysis of MYB103 Transcription Factor in Wolfberry[J].Northern Horticulture,2012,36(18):118.
[5]郭涛１,２,晁跃辉,等.拟南芥NCED4基因的克隆及初步功能鉴定[J].北方园艺,2014,38(17):105.
　GUO Tao,CHAO Yue-hui,et al.Cloning and Transformation of NCED4 Gene from Arabidopsis thaliana[J].Northern Horticulture,2014,38(18):105.
[6]王 崇,程玉祥.AtGDPD-Like 6和AtGDPD-Like 7基因启动子表达特性及AtGDPD-Like 6蛋白定位[J].北方园艺,2015,39(11):90.[doi:10.11937/bfyy.201511022]
　WANG Chong,CHENG Yu-xiang.The Expression Patterns of Arabidopsis thaliana GDPDL6 and GDPDL7 and the Subcellular Localization of GDPDL6[J].Northern Horticulture,2015,39(18):90.[doi:10.11937/bfyy.201511022]
[7]杨淑萍,马伟琴,刘阳,等.模式生物拟南芥研究进展的文献计量分析[J].北方园艺,2015,39(19):195.[doi:10.11937/bfyy.201519049]
　YANG Shuping,MA Weiqin,LIU Yang,et al.Bibliometric Analysis of Research Progress on Model Species Arabidopsis thaliana[J].Northern Horticulture,2015,39(18):195.[doi:10.11937/bfyy.201519049]
[8]穆红梅,杜秀菊,张秀省,等.植物MYB转录因子调控苯丙烷类生物合成研究[J].北方园艺,2015,39(24):171.[doi:10.11937/bfyy.201524046]
　MU Hongmei,DU Xiuju,ZHANG Xiusheng,et al.Study on Plants MYB Transcription Factors Regulate Biological Synthesis of Phenylpropanoid Metabolism[J].Northern Horticulture,2015,39(18):171.[doi:10.11937/bfyy.201524046]
[9]刘璐,肖志娜,刘菲,等.矮牵牛花香生物合成及其调控研究进展[J].北方园艺,2016,40(01):181.[doi:10.11937/bfyy.201601047]
　LIU Lu,XIAO Zhina,LIU Fei,et al.Floral Scent Biosynthesis and Its Regulation in Petunia[J].Northern Horticulture,2016,40(18):181.[doi:10.11937/bfyy.201601047]
[10]周 　 索,杜 　 丽.四种生长素对拟南芥根生长发育的影响[J].北方园艺,2008,32(03):0.[doi:10.11937/bfyy.200803024]
　ZHOU Suo,DU Li.Study on the Root Growth and Development of Arabidopsis thaliana[J].Northern Horticulture,2008,32(18):0.[doi:10.11937/bfyy.200803024]

备注/Memo

第一作者简介：唐尧（1992-），男，硕士研究生，研究方向为植物资源学与分子植物学。E-mail：2427472516@qq.com.责任作者：常缨（1970-），女，博士，教授，博士生导师，研究方向为植物资源学与分子生物学。E-mail：changying@neau.edu.cn.基金项目：国家自然科学基金资助项目（31370221）。收稿日期：2018-03-22

更新日期/Last Update: 2018-10-16