[1]侯丽霞,王文杰,刘新.葡萄VvWRKY13转录因子的生物信息学分析[J].北方园艺,2013,37(13):117-120.
　HOU Li-xia,WANG Wen-jie,LIU Xin.Bioinformatics Analysis of VvWRKY13 Transcription Factor in Vitis vinifera L.[J].Northern Horticulture,2013,37(13):117-120.
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葡萄VvWRKY13转录因子的生物信息学分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年13期 页码: 117-120 栏目: 出版日期: 2013-07-15
Title:
Bioinformatics Analysis of VvWRKY13 Transcription Factor in Vitis vinifera L.
文章编号:
1001-0009(2013)13-0117-04
作者:
侯丽霞; 王文杰; 刘新
青岛农业大学 生命科学学院，山东省高校植物生物技术重点实验室，山东 青岛 266109
Author(s):
HOU Li-xia; WANG Wen-jie; LIU Xin
Key Lab of Plant Biotechnology in Universities of Shandong Province，College of Life Science，Qingdao Agricultural University，Qingdao，Shandong 266109
关键词:
葡萄; VvWRKY13; 转录因子; 生物信息学
Keywords:
Vitis vinifera L.; VvWRKY13; transcription factor; bioinformatic
分类号:
Q 946.1；S 663.1
文献标志码:
A
摘要:
利用生物信息学方法对葡萄VvWRKY13基因编码蛋白的理化性质、保守结构域、亲水性/疏水性、信号肽、跨膜结构域、磷酸化修饰、二级结构、亚细胞定位和系统进化等进行了预测和分析。结果表明：该蛋白分子量为25 718.1 Da，等电点为9.08，不稳定系数为60-78；C端含有WRKY保守域，属于WRKY转录因子家族成员；不具有信号肽和跨膜域，是亲水性蛋白；蛋白质二级结构最主要的结构元件是α螺旋和无规则卷曲，并散布于整个蛋白；亚细胞定位预测显示该蛋白主要位于细胞核。该研究结果将为进一步研究其生物学功能提供参考。
Abstract:
The characteristic of VvWRKY13 transcription factor in grape was analyzed and predicted by bioinformatic methods in the following aspects：physical and chemical characters，conserved domain，hydrophobicity or hydrophilicity，signal peptide，transmembrance structure，phosphorylation in amino acid，secondary and tertiary structure of protein，subcellular localization and molecular phylogenetic evolution.The results showed that VvWRKY13 had molecular weight 25 718.1 Da，isoelectric point 9.08 and instability coefficient 60.87；it possessed one conserved WRKY domain，so it was Ⅱ type WRKY transcription factor；VvWRKY was a hydrophilicity protein without a signal peptide and transmembrance structure；random coil and αhelix were the major structural elements of polypeptide chain；subcellular localization result indicated that VvWRKY13 was located in nucleus very likely.This research would be of great information and values for its function study.

参考文献/References:

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备注/Memo

第一作者简介：侯丽霞（1978-），女，博士，讲师，研究方向为葡萄分子细胞生物学。E-mail:houlixia78@163.com.

责任作者：刘新（1966-），女，博士，教授，研究方向为植物逆境生理与分子生物学。E-mail:liuxin6080@yahoo.com.cn.
基金项目：青岛市自然科学基金资助项目（12--1-4-5-(12)jch；10-3-4-5-5-jch）；青岛农业大学高层次人才启动基金资助项目（630722）。收稿日期：2013-03-04

更新日期/Last Update: 2014-08-08