ZHANG Shuihua,LI Benhong,ZHOU Weidong.Ultrasonic-assisted Extraction and Antioxidant Activity of Polysaccharides From Laccaria laccata[J].Northern Horticulture,2018,42(17):133-138.[doi:10.11937/bfyy.20174106]
红蜡蘑多糖的超声波辅助提取工艺及其体外抗氧化性研究
- Title:
- Ultrasonic-assisted Extraction and Antioxidant Activity of Polysaccharides From Laccaria laccata
- Keywords:
- Laccaria laccata; polysaccharides; ultrasonic; DPPH·; ABTS+·
- 文献标志码:
- A
- 摘要:
- 以红蜡蘑为试材，研究了液料比、超声功率、超声时间、超声温度、提取次数对多糖得率的影响，在单因素试验基础上，采用正交实验优化了工艺条件，对提取产物多糖进行了DPPH·、ABTS+·清除研究。结果表明：对多糖提取率影响最大的是提取次数，其次是液料比，提取时间影响最小；最佳提取工艺条件为提取3次，液料比20∶1 mL·g-1，超声功率300 W，超声温度70 ℃，超声时间40 min，在此条件下多糖提取率达6.27%。多糖对DPPH·、ABTS+·有一定的清除作用，说明红蜡蘑多糖具有一定的抗氧化活性。
- Abstract:
- Taking Laccaria laccata as the raw material,the effects of extraction conditions such as ratio of liquid-solid,ultrasonic time,ultrasonic power,ultrasonic temperature and time of extraction were investigated by one-factor tests and orthogonal test,and the extraction polysaccharides were analyzed with DPPH· and ABTS+· test.The results showed that the times of extraction affected the yield markably,the second was ratio of liquid-solid,and ultrasonic time had the least influence;the optimum coditions were determined as follows:times of extraction of 3 times,ratio of liquid-solid of 20∶1 mL·g-1,ultrasonic power of 300 W,ultrasonic temperature of 70 ℃ and ultrasonic time of 40 minutes.Under the condition,the yield was up to 6.27%.Polysaccharides exhibited antioxidanteffects in 2,2′-diphenyl-1-picrylhydrazyl(DPPH·),2,2′-azino-bis(3-ethylben-othiazoline-6-sulfonic acid(ABTS+·) radical scavenging assay.And it showed that the extraction polysaccharides which had some oxidation resistance.
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备注/Memo
第一作者简介：张水花（1980-），女，硕士，副教授，研究方向为天然产物化学及保健食品。E-mail:z_shh@163.com.基金项目：云南省科技厅研究基金资助项目（2016FD081）。收稿日期：2018-03-28