WANG Jing,ZI Hongzhou,LI Huangwei,et al.Effects of Salt Stress on Stipa breviflora Seedling and Antioxidant Isozyme[J].Northern Horticulture,2018,42(14):109-117.[doi:10.11937/bfyy.20174088]
盐胁迫对短花针茅幼苗生长及抗氧化同工酶的影响
- Title:
- Effects of Salt Stress on Stipa breviflora Seedling and Antioxidant Isozyme
- Keywords:
- Stipa breviflora; salt stress; SOD; POD; CAT
- 文献标志码:
- A
- 摘要:
- 以从内蒙古境内采集的短花针茅种子为试材，设定0、50、100、150、200 mmol·L-1 NaCl 5个浓度处理，研究盐胁迫对短花针茅幼苗生长及抗氧化酶的影响，以期深入理解短花针茅对盐胁迫的响应机制，为其深化应用提供理论依据。结果表明：随着NaCl浓度的增大，短花针茅发芽率、根长及幼苗鲜质量会受到不同程度的影响。发芽率、根长及幼苗鲜质量受明显抑制的浓度分别为100、150、50 mmol·L-1，数值分别为62%、1.30 cm和0.299 g。随着NaCl浓度的增大，短花针茅幼苗超氧化物歧化酶（SOD）及过氧化物酶（POD）先升高后降低，分别于150、100 mmol·L-1 时达到最大值，分别为48.6 U·g-1和6 766 U·g-1；过氧化氢酶（CAT）随NaCl浓度增大酶活性逐渐增强，150 mmol·L-1 时达到最大值299 U·g-1。同工酶酶谱显示SOD 2～4，POD 1～2以及CAT 1～2，随NaCl浓度增大表达量增加，与短花针茅抵御盐胁迫密切相关。
- Abstract:
- Stipa breviflora seeds,collected from Inner Mongolia,were sowed on MS medium containing 0,50,100,150,200 mmol·L-1NaCl,to investigate the effect of salt stress on the physiological phenotype and antioxidant enzymes activity of Stipa breviflora seedling.The results showed the germination rate,root length and fresh weight of seedling [JP2]were all influenced by salinity.Germination rate,root length and fresh weight of seedling were significantly inhibited by 100,150 and 50 mmol·L-1 NaCl and data were 62%,1.30 cm,0.299 g respectively.With NaCl content increasing,the tread of superoxide dismutase (SOD) and peroxidase (POD) activity were first rising and then falling.When the NaCl concentration reached 150 and 100 mmol·L-1,the activity of SOD and POD were the maximum,48.6 U·g-1 and 6 766 U·g-1 respectively.The activity of catalase (CAT) was continuing increased and reached the maximum at 100 mmol·L-1,was 299 U·g-1.The isozyme showed SOD 2-4,POD 1-2,and CAT 1-2 were positively correlated with salinity tolerance of Stipa breviflora seedling.
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WANG Jing,ZI Hongzhou,LI Huangwei,et al.Effects of Salt Stress on Stipa breviflora Seedling and Antioxidant Isozyme[J].Northern Horticulture,2018,42(14):77.[doi:10.11937/bfyy.20174088]
备注/Memo
第一作者简介：王静（1984-），女，博士，讲师，现主要从事植物逆境生物化学与分子生物学等研究工作。E-mail：wangjing_imu@163.com.基金项目：北方民族大学引进人员科研启动资助项目（4400302550）；国家自然科学基金资助项目（31600202）。收稿日期：2018-03-01