JIANG Tong,FU Xiang,WANG Hui,et al.Flower Morphological and Physiological Characters Between Male Sterile Line and Maintainer Line in Cluster Pepper[J].Northern Horticulture,2018,42(11):22-26.[doi:10.11937/bfyy.20173823]
簇生朝天椒雄性不育系及保持系的花器官及生理特性研究
- Title:
- Flower Morphological and Physiological Characters Between Male Sterile Line and Maintainer Line in Cluster Pepper
- Keywords:
- cluster pepper; CMS; flower; energy substance; protective enzyme
- 文献标志码:
- A
- 摘要:
- 以簇生椒不育系‘001A’和同型保持系‘002B’为材料，观察花器官的形态差异，研究不同器官和不同花蕾发育时期不育系与保持系的能量物质含量和保护酶活性的差异，以探索CMS型簇生椒物质代谢与雄性不育之间的关系，从理化方面研究簇生朝天椒不育系‘001A’的败育机理。结果表明：不育系‘001A’ 花药干瘪、无花粉、花器比正常花器小、柱头露出花药，而保持系‘002B’花药饱满，柱头不外露。不同植株器官和不同开花时期均存在保持系‘002B’中的游离脯氨酸、可溶性糖、蛋白质含量高于不育系‘001A’；各个开花时期不育系‘001A’的丙二醛含量高于保持系‘002B’，植株茎和叶的丙二醛含量表现则相反。辣椒不同植株器官均存在保持系‘002B’中的CAT活性高于不育系‘001A’，SOD活性表现则相反；在各个开花时期不育系‘001A’的POD、SOD活性均显著高于保持系‘002B’，而不育系‘001A’的CAT活性显著低于保持系‘002B’。因此，不育系花药中营养物质的缺乏和保护酶间的异常，是导致簇生朝天椒 CMS花粉败育的关键。
- Abstract:
- In order to explore the relationship between CMS metabolism and male sterility,the mechanism of the failure of the ‘001A’ was studied from the physiological and biochemical aspects.The cluster pepper sterile line ‘001A’ and their corresponding maintaine lines ‘002B’ were chosen as experimental material,either observations of both buds and anthers the cluster pepper were carried out,the changes of the content of energy substance and protective enzyme were determined at different development stage and organ.The results showed that,sterile line ‘001A’ was dry,no pollen and the flower device was smaller than the normal flower and the stigma was exposed to the anthers,the ‘002B’ was full and the stigma was not exposed.The contents of proline,soluble sugar and protein in sterile line ‘001A’ was significantly lower than that of in maintainer line ‘002B’ were determined at different development stage and organ.Malondialdehyde in each flowering period were higher in sterile lines‘001A’ than maintainer lines ‘002B’,whereas malondialdehyde in plant stems and leaves vice versa.The activity of CAT in sterile line ‘001A’ was significantly lower than that of in maintainer line ‘002B’ were determined at different development organ,where as SOD in different development organ vice versa.The activities of POD and SOD in each flowering period were higher in sterile lines ‘001A’than maintainer lines ‘002B’,but the specific activity of CAT in sterile line ‘001A’ significantly lower than that of in maintainer line ‘002B’.Therefore，the deficiency of nutritive substances in sterile line anthers and the abnormality of protective enzymes were the key to the pollen abortion of the cluster.
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备注/Memo
第一作者简介：姜童（1995-），男，硕士研究生，研究方向为蔬菜遗传育种与分子生物学。E-mail：583944745@qq.com.责任作者：林多（1973-），女，博士，教授，硕士生导师，现主要从事蔬菜遗传育种与分子生物学等研究工作。E-mail：linduo73@163.com.基金项目：山东省农业良种工程资助项目（2016LZGC011）；山东省农业重大应用技术创新资助项目（682214007）；山东省自然科学基金资助项目（ZR2014CQ034）。收稿日期：2018-03-01