[1]苑克俊,牛庆霖,葛福荣,等.利用荧光SSR标记构建杏新品系的分子身份证[J].北方园艺,2018,42(04):34-40.[doi:10.11937/bfyy.20172966]
　YUAN Kejun,NIU Qinglin,GE Furong,et al.Molecular Identity Establishment of Several Apricot Strains Based on Fluorescent Labeled SSR Markers[J].Northern Horticulture,2018,42(04):34-40.[doi:10.11937/bfyy.20172966]
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利用荧光SSR标记构建杏新品系的分子身份证

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年04 页码: 34-40 栏目: 出版日期: 2018-02-28
Title:
Molecular Identity Establishment of Several Apricot Strains Based on Fluorescent Labeled SSR Markers
作者:
苑克俊; 牛庆霖; 葛福荣; 王江勇; 王培久
山东省果树研究所，山东 泰安 271000
Author(s):
YUAN Kejun; NIU Qinglin; GE Furong; WANG Jiangyong; WANG Peijiu
Shandong Institute of Pomology，Tai′an,Shandong 271000
关键词:
杏; 种质; SSR标记; 荧光引物; 分子身份证
Keywords:
apricot; germplasm; SSR marker; fluorescent labeled primer; molecular identity
DOI:
10.11937/bfyy.20172966
文献标志码:
A
摘要:
杏；种质；SSR标记；荧光引物；分子身份证
Abstract:
The 15 germplasms （including 4 new strains) and 6 SSR markers with fluorescent labeled primers were used to perform PCR experiment,the molecular identities of 4 strains selected from hybrid apricot plants were established.The purpose of this study was to provide experiment basis for cultivar right protection of 4 apricot strains using molecular identification.The results showed that a total of 47 polymorphic alleles and 57 electrophoresis patterns were revealed by the 6 markers，with an average of 7.8 alleles and 9.8 patterns for each marker；and the molecular identities of 4 strains were different from each other and other 11 germplasms.These results provided experiment basis for cultivar right protection of 4 apricot strains using molecular identification.

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备注/Memo

第一作者简介：苑克俊（1963-），男，博士，研究员，现主要从事杏树栽培育种及果树基因与分子标记等研究工作。E-mail：yuankj@vip.sina.com.

基金项目：山东省农业良种工程资助项目（鲁科字[2014]96）；泰安市科技发展计划资助项目（2015NS1067）；山东省农业科学院农业科技创新工程资助项目。
收稿日期：2017-09-30

更新日期/Last Update: 2018-02-23