YANG Qin,CHEN Lei,ZHANG Tingting,et al.Effects of Flower Age on Pollen Germination and Pollen Tube Growth of ‘Britewell’ Rabbitteye Blueberry[J].Northern Horticulture,2018,42(09):57-61.[doi:10.11937/bfyy.20172906]
花龄对‘灿烂’兔眼蓝莓花粉原位萌发和花粉管生长的影响
- Title:
- Effects of Flower Age on Pollen Germination and Pollen Tube Growth of ‘Britewell’ Rabbitteye Blueberry
- 文献标志码:
- A
- 摘要:
- 以‘灿烂’兔眼蓝莓为试材，分别进行异花授粉和自花授粉后，于去雄后第0、1、2、3、4、5、6天对去雄的花进行授粉，于授粉后2、6、12、24、48、72、96 h进行取样观察花粉原位萌发和花粉管生长情况，以期阐明花龄对蓝莓花粉萌发和花粉管生长的影响，为杂交育种和花期人工辅助授粉提供科学依据。结果表明：‘灿烂’兔眼蓝莓开花第1～4天，异花授粉后花粉管生长至花柱基部的花柱比率显著高于其它时期，花龄对其异花和自花授粉后花粉原位萌发和花粉管生长有着显著的影响，且‘灿烂’兔眼蓝莓存在自交不亲和的现象，花柱基部为其自交不亲和的关键位置，大蕾期和延迟授粉可以一定程度地克服自交不亲和现象，人工辅助授粉和杂交育种的最佳授粉时间为花后第1～4天。
- Abstract:
- In this study，using the ‘Britewell’ as test materials，the pollen germination and pollen tube growth of self-pollination and cross-pollination after detasseled 0，1，2，3，4，5 days and 6 days were investigated，and the flowers pollinated were sampled 2，6，12，24，48，72 and 96 hours after pollination.So as to elucidate effects of flower age on pollen germination，pollen tube growth and fruit set of Rabbitteye blueberry，to provide knowledge for artificial supplementary pollination and crossbreeding.The results showed that there was a significant difference in pollen germination and pollen tube growth and fruit set among different growth-phase flowers of self-pollination or cross-pollination.In 1-4 days after flowering,the rate of style traversed by pollen tubes of flowers was significantly higher than other growth-phase flowers.Meanwhile，the self-incompatibility was observed in ‘Britewell’，and the base of style was the key position of self-incompatibility，and the self-incompatibility was reduced to a certain extent by bud pollination and delayed pollination.In short，it was the 1-4 days after flowering that the best time of artificial supplementary pollination and crossbreeding.
参考文献/References:
[1]李亚东,刘海广,张志东,等.中国蓝莓产业现状和发展趋势[J].中国果树,2008,28(6):67-69,71. [2]王乾,冷吉燕,郑恩来,等.蓝莓功效的研究进展[J].中国老年学杂志,2015(14):4082-4084. [3]李亚东,孙海悦,陈丽,等.我国蓝莓业发展报告[J].中国果树,2016(5):1-10. [4]徐兆丰,于晓伟,许文敏.蓝莓建园栽植及科学采收技术[J].中国园艺文摘,2012(11):161-163. [5]杨芩,李性苑,田鑫,等.‘粉蓝’兔眼蓝莓适宜授粉品种的筛选[J].北方园艺,2015(6):5-7. [6]杨芩,李性苑,田鑫,等.花粉直感对‘杰兔’兔眼蓝莓着果率和果实品质的影响[J].中国南方果树,2015,44(4):70-72. [7]杨芩,付燕,王永清,等.4个枇杷品种S基因型鉴定及新基因S31-RNase序列分析[J].果树学报,2015,32(1):37-42. [8]SASSA H.Molecular mechanism of the S-RNase-based gametophytic self-incompatibility in fruit trees of Rosaceae[J].Breeding Science,2016,(66):116-121. [9]杨芩,付燕.果树自交不亲和SFB与S-RNase作用分子机制研究进展[J].中国果树,2015(2):70-73. [10]李晓,吴华清,吴俊,等.不同发育阶段甜樱桃花授粉后花粉萌发和花粉管生长的差异[J].果树学报,2008,25(4):501-505. [11]陈迪新,张绍铃.梨不同花龄自花与异花授粉的花柱内花粉管生长及坐果率的比较[J].果树学报,2007,24(5):575-579. [12]杨芩,任永权,廖优江,等.花龄对蓝莓柱头可授性及花粉活力的影响[J].中国南方果树,2012,41(5):25-27. [13]杨芩,付燕,王永清,等.温度对枇杷花粉管生长和胚囊寿命的影响[J].果树学报,2015,32(4):646-652. [14]谷超.李属（PRUNUS）植物自交亲和性及S基因进化机制的研究[D].南京:南京农业大学,2012.
相似文献/References:
[1]王明洁,吴雨蹊,段亚东,等.蓝莓栽培土壤改良技术研究[J].北方园艺,2014,38(14):179.
WANG Ming-jie,WU Yu-xi,DUAN Ya-dong,et al.Improvement Research of Technique on Cultivation Soil of Blueberry[J].Northern Horticulture,2014,38(09):179.
[2]王贺春.蓝莓冬季修剪技术[J].北方园艺,2013,37(13):58.
WANG He-chun.Winter Pruning Techniqne of Blueberry[J].Northern Horticulture,2013,37(09):58.
[3]聂飞,张玉春.我国蓝莓产业发展的机遇与挑战[J].北方园艺,2014,38(04):165.
NIE Fei,ZHANG Yu-chun.Opportunities and Challenges for the Development of Blueberry Industry in China[J].Northern Horticulture,2014,38(09):165.
[4]傅 冰,洪 震,刘跃钧,等.蓝莓SRAP-PCR反应体系的建立和优化及其遗传多样性分析[J].北方园艺,2013,37(17):95.
FU Bing,HONG Zhen,LIU Yue-jun,et al.Optimization and Establishment of SRAP-PCR Reaction System of Vaccinium ssp. and Analysis of the Genetic Diversity[J].Northern Horticulture,2013,37(09):95.
[5]张文英,周 婧.低糖蓝莓番茄复合果酱的研制[J].北方园艺,2013,37(17):142.
Brewing Technology Engineering Research Center,Department of Food Science,Jilin Agricultural Science and Technology College,et al.Preparation of Low-sugar Jam with Blueberry and Tomato[J].Northern Horticulture,2013,37(09):142.
[6]龚 娜,肖 军,杨 镇,等.不同菌根真菌对蓝莓根际土壤微生物数量的影响[J].北方园艺,2013,37(17):175.
GONG Na,XIAO Jun,YANG Zhen,et al.Effects of Different Mycorrhizal Fungi on Soil Microbial Number in the Blueberry Rhizosphere[J].Northern Horticulture,2013,37(09):175.
[7]王贺春.适于丹东地区温室栽培的蓝莓引种与筛选[J].北方园艺,2013,37(12):33.
WANG He-chun.Introduction and Selection of Blueberry Varieties for Greenhouse That Suitable for Dandong Region[J].Northern Horticulture,2013,37(09):33.
[8]吴立仁,段亚东,杜汉军,等.黑龙江省蓝莓种植应该注意的几个问题[J].北方园艺,2013,37(05):50.
WU Li-ren,DUAN Ya-dong,DU Han-jun,et al.Several Problems Existing in Planting of Blueberry in Heilongjiang Province[J].Northern Horticulture,2013,37(09):50.
[9]刘 波.果胶酶对蓝莓果汁澄清效果的研究[J].北方园艺,2014,38(03):127.
LIU Bo.Study on Clarification Effect of Pectinase on Blueberry Juice[J].Northern Horticulture,2014,38(09):127.
[10]刘娥,郭军,戴文佳,等.植物激活蛋白可湿性粉剂调节蓝莓生长试验[J].北方园艺,2012,36(02):159.
LIU E,GUO Jun,DAI Wen-jia,et al.The Test of Plant-activated Protein WP to Improve Blueberry Growth[J].Northern Horticulture,2012,36(09):159.
[11]杨芩,任永权,廖优江,等.五个兔眼蓝莓品种有效可授期研究[J].北方园艺,2013,37(14):5.
YANG Qin,REN Yong-quan,LIAO You-jiang,et al.Study on Effective Pollination Period of Five Cultivars of Rabbiteye Blueberry[J].Northern Horticulture,2013,37(09):5.
备注/Memo
第一作者简介：杨芩（1983-），男，博士，教授，现主要从事果树生长发育系统调控与生态栽培技术等研究工作。E-mail：yangqin1028518@126.com.基金项目：凯里学院2016年度科技合作协议资助项目（黔科合HL字[2016]7331号）；贵州省教育厅“125计划”重大科技专项资助项目（黔教合重大专项字[2013]028）；凯里学院院级规划资助项目 (Z1302)；贵州省教育厅自科资助项目（黔教合KY字[2016]303）。收稿日期：2018-02-23