[1]张淑颖,曲田丽,赵川德.JM-3菌株抗菌物质发酵条件优化及对苹果腐烂病的防效[J].北方园艺,2018,42(04):54-59.[doi:10.11937/bfyy.20171622]
　ZHANG Shuying,QU Tianli,ZHAO Chuande.Optimizing Fermentation Condition for Antagonistic JM-3 Strain and Protection Effect Against Valsa ceratosperma[J].Northern Horticulture,2018,42(04):54-59.[doi:10.11937/bfyy.20171622]
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JM-3菌株抗菌物质发酵条件优化及对苹果腐烂病的防效

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年04 页码: 54-59 栏目: 出版日期: 2018-02-28
Title:
Optimizing Fermentation Condition for Antagonistic JM-3 Strain and Protection Effect Against Valsa ceratosperma
作者:
张淑颖1; 曲田丽2; 赵川德1
1.青岛农业大学 植物保护学院，山东 青岛 266109；2.青岛农业大学 化学与药学院，山东 青岛 266109
Author(s):
ZHANG Shuying1; QU Tianli2; ZHAO Chuande1
1.College of Plant Protection,Qingdao Agricultural University,Qingdao,Shandong 266109；2.College of Chemistry and Pharmaceutical Sciences,Qingdao Agricultural University,Qingdao,Shandong 266109
关键词:
抗菌物质; 发酵条件; 防病作用; 苹果腐烂病
Keywords:
antifungal substances; fermentation condition; disease protection; Valsa ceratosperma
DOI:
10.11937/bfyy.20171622
文献标志码:
A
摘要:
以从剑麻中筛选出的1株对苹果腐烂病有较强拮抗作用的菌株JM-3为研究对象，采用单因子试验和正交实验对菌株JM-3的最适发酵培养基成分及发酵条件进行优化，并测定了其对苹果离体枝条腐烂病的防治效果，以期为该菌株的工业化生产提供参考依据。结果表明：菌株JM-3培养基各组分最佳配比为葡萄糖30 g?L-1，蛋白胨20 g?L-1，K2HPO4 1.0 g?L-1，最佳发酵条件为初始pH 7，装液量150 mL或100 mL/250 mL三角瓶，培养温度33 ℃，培养时间120 h。在最佳发酵培养基和培养条件下，菌株JM-3抑菌能力提高32.4%。JM-3发酵液不能对苹果离体枝条腐烂病起到治疗作用，但能够显著预防腐烂病斑扩展，提前24~48 h接种JM-3发酵液的预防保护效果最好，与对照农药苯醚甲环唑相比无显著差异。
Abstract:
Strain JM-3 isolated from sisal was used as study object,the single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium and fermentation conditions of JM-3,and the control effect on apple excised twigs against Valsa ceratosperma was tested,in order to provide reference for establishing industrial production.The results showed that optimum medium was glucose 30 g?L-1,peptone 20 g?L-1，K2HPO4 1.0 g?L-1.The optimal fermentation conditions were initial pH 7,medium capacity 150 mL or 100 mL/250 mL,temperature 33 ℃,fermentation time 120 hours.Inhibitory activity of strain JM-3 fermentation solution increased 32.4% under the optimum fermentation medium and culture conditions.The fermentative liquid of strain JM-3 could not cure canker lesion expansion,but it could prevent canker lesion expansion significantly.The preventive protection of strain JM-3 fermentative liquid was the best when it was inoculated on apple twigs before 24-48 hours,and its protective effect had no significant difference with difenoconazole.

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备注/Memo

第一作者简介：张淑颖（1980-)，女，博士，实验师，研究方向为植物病害的生物防治。E-mail:shuyingzhang@163.com.

基金项目：青岛农业大学高层次人才科研基金资助项目（1116028）。
收稿日期：2017-10-18

更新日期/Last Update: 2018-02-24