WANG Yan,ZHANG Guifang,YAN Xiaoqiao,et al.Biological Characteristics of Glomerella cingulate From Dendrobium officinale[J].Northern Horticulture,2018,42(11):61-66.[doi:10.11937/bfyy.20171282]
铁皮石斛围小丛壳菌的生物学特性
- Title:
- Biological Characteristics of Glomerella cingulate From Dendrobium officinale
- 文献标志码:
- A
- 摘要:
- 以近期分离鉴定的铁皮石斛炭疽病原新纪录种铁皮石斛围小丛壳菌（Glomerella cingulata）为试材，采用组织培养的方法系统研究了铁皮石斛围小丛壳菌的生物学特性。以期找到高效快速的防治方法，以解决铁皮石斛炭疽病频繁发生且有日益加重趋势的问题。结果表明：铁皮石斛围小丛壳菌菌丝适应性强，在不同培养基均能生长，在PDA培养基上生长最快；在10~35 ℃下均可以生长,25~30 ℃下较适合菌丝体的生长，28 ℃菌丝生长最快，产子囊壳最多；不同光照条件下生长均较好，但光照可促进子囊壳的产生；在pH 5~12下均可生长，但pH 8时菌丝生长最快，pH 9时子囊壳产量最高；在不同碳氮源上均可生长，山梨醇对菌丝的生长最有利，在麦芽糖碳源上菌丝浓密，子囊壳的产量达到最高；菌丝对蛋白胨氮源的利用率最高。菌丝体的水浴致死条件是50 ℃。
- Abstract:
- Glomerella cingulata is a new record species which was recently isolated anthrax pathogen from Dendrobium officinale,and was used as a test material.The biological characteristics of Glomerella cingulata were studied by the method of tissue culture,in order to find efficient and rapid prevention and control methods.To solve the problem of frequent occurrence of anthrax of Dendrobium officinale and its increasing trend.The results showed that the adaptability of Glomerella cingulata was strong and could grow on different medium.PDA was suitable for mycelial growth.It could grow at 10-35 ℃,and it was suitable for the growth of mycelium at 25-30 ℃.When the temperature was 28 ℃,the dense mycelia grew the fastest and produced the highest perithecia.The growth was better under different light conditions.But the light could promote the perithecia.It could grow under pH 5-12,but mycelium growth rate was the fastest when the pH was 8.The yield of perithecia was the highest when the pH was 9.It could be grown on different carbon and nitrogen sources.Sorbitol was the best for mycelia growth.The mycelia was dense in the maltose carbon source media and perithecia production reached the highest.Mycelia had the highest efficiency using to peptone.The water bath lethal temperature of mycelium was 50 ℃.
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备注/Memo
第一作者简介：王艳（1990-），女，硕士研究生，研究方向为中药资源与开发。E-mail：1479723955@qq.com.责任作者：张桂芳（1980-），女，河南开封人，博士，副教授，现主要从事中药资源开发利用等研究工作。E-mail：zhanggf@gzucm.edu.cn.基金项目：广东省省级科技计划科技基础条件建设领域资助项目（2014A030304059）；广东省省级科技计划科技应用型科技研发专项资金资助项目（2015B020234008）；国家自然科学基金资助项目（81001601）；广东省优秀青年教师培养计划资助项目（yq2014042）；粤港澳岭南中药综合开发研究资助项目（2014DFH30010）。收稿日期：2017-07-18