QIAO Wei,CHEN Wenqiang,PENG Hao,et al.Optimization of Extraction Process of Response From Selenium-polysaccharide Enriched Lentinus edodes in Fermentation Liquid Surface[J].Northern Horticulture,2017,41(12):157-162.[doi:10.11937/bfyy.201712035]
富硒香菇发酵液中硒多糖提取工艺的响应面优化
- Title:
- Optimization of Extraction Process of Response From Selenium-polysaccharide Enriched Lentinus edodes in Fermentation Liquid Surface
- 关键词:
- 香菇; 硒多糖; Box-Behnken设计; 回归模型分析; 响应面分析
- Keywords:
- Lentinus edodes; selenium-polysaccharide; Box-Behnken design; regression model anaylsis; response surface anaylsis
- 文献标志码:
- A
- 摘要:
- 以香菇9608#菌株为试材，在单因素试验基础上，采用Box-Behnken响应面设计法优化香菇发酵液中硒多糖提取工艺。结果表明：富硒香菇发酵液中提取工艺硒多糖最佳醇沉时间为15.45 h、乙醇浓度82.30%、pH 7.95。在此条件下，富硒香菇发酵液中硒多糖产量达到3.23 g?L-1，香菇发酵液中硒多糖硒含量87.67 μg?g-1。利用Box-Behnken响应面设计法得到的富硒香菇发酵液中硒多糖提取工艺参数，可为工业化生产提供必要的技术支持。
- Abstract:
- Based on the single factor experiment,the method of Box-Behnken response surface design was used to optimize the extraction process of selenium-polysaccharide in Lentinus edodes fermentation broth.The results showed that the optimum alcohol extraction time of selenium-polysaccharide was 15.45 hours,ethanol concentration 82.30% and pH 7.95.Under these conditions,the yield of selenium-polysaccharide in the fermentation broth of selenium-enriched Lentinus edodes was 3.23 g?L-1,and the selenium content of selenium-polysaccharide was 87.67 μg?g-1.Using the Box-Behnken response surface design method,the extraction parameters of selenium-polysaccharide from the selenium-enriched Lentinus edodes fermentation broth could provide the necessary technical support for the industrial production.
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备注/Memo
第一作者简介：乔伟(1992-)，男，硕士研究生，研究方向为微生物资源利用开发。E-mail:838232966@qq.com.责任作者：陈文强(1956-)，男，教授，硕士生导师，现主要从事微生物资源保护与利用等研究工作。E-mail:wenqiangc@126.com.基金项目：陕西省“13115”科技创新工程计划资助项目(2008ZDGC-04)。