[1]赖黎丽,张嘉园,杨梅,等.新疆加工型辣椒花药培养技术研究[J].北方园艺,2017,41(19):6-10.[doi:10.11937/bfyy.20170971]
　LAI Lili,ZHANG Jiayuan,YANG Mei,et al.Technological Study of Processing Pepper Anther Culture in Xinjiang[J].Northern Horticulture,2017,41(19):6-10.[doi:10.11937/bfyy.20170971]
点击复制

新疆加工型辣椒花药培养技术研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年19 页码: 6-10 栏目: 出版日期: 2017-10-15
Title:
Technological Study of Processing Pepper Anther Culture in Xinjiang
作者:
赖黎丽1; 2; 张嘉园1; 杨梅1; 宋文胜2; 朱华国1
1.石河子大学 农学院，新疆 石河子 832000；2.新疆隆平高科红安种业有限责任公司，新疆 石河子 832000
Author(s):
LAI Lili1; 2; ZHANG Jiayuan1; YANG Mei1; SONG Wensheng2; ZHU Huaguo1
1.College of Agronomy,Shihezi University,Shihezi,Xinjiang 832000；2.Xinjiang Longping High-Tech Hongan Seeds Co.Ltd.，Shihezi,Xinjiang 832000
关键词:
辣椒; 花药培养; 胚状体
Keywords:
pepper; anther culture; embryo
DOI:
10.11937/bfyy.20170971
文献标志码:
A
摘要:
以6个新疆加工型辣椒品种207、208、64、66、67、HT113为试材，以MSB培养基为基本培养基，附加不同植物激素浓度的NAA、2,4-D、TDZ、KT进行花药培养，研究不同植物激素处理对加工型辣椒花药愈伤组织和胚状体诱导的影响，以建立适应于新疆加工型辣椒的花药培养技术。结果表明：不同基因型辣椒的愈伤组织诱导率和出胚率均不同，愈伤组织诱导率为4.98%~29.65%，胚状体诱导率为0~6.88%。0.5 mg?L-1 2,4-D+0.1 mg?L-1 TDZ和 0.5 mg?L-1 NAA+0.1 mg?L-1 KT分别是愈伤组织诱导和胚状体诱导最适宜的激素组合；在新疆特有的气候条件和种植模式下，7月中旬前的花药接种培养最为适宜；胚状体继续发育成熟后，通过练苗移栽获得了56株单倍体植株。
Abstract:
Six processing pepper varieties 207,208,64，66，67 and TH113 in Xinjiang were used for anther culture.The anthers in different stages were induced in basic MSB medium supplemented with NAA,2,4-D,TDZ and KT in various concentrations.Effects of different hormone treatment on processing pepper anther culture were studied,and established a suitable anther culture method for processing pepper in Xinjiang.The results showed that different ratio of callus induction and embryo induction emerged in different genotype peppers,the ratio of callus induction were 4.98%-29.65% and the ratio of embryo induction were 0-6.88%.0.5 mg?L-1 2,4-D+0.1 mg?L-1 TDZ and 0.5 mg?L-1 NAA+0.1 mg?L-1 KT were the suitable hormone combination for callus induction and embryo induction,respectively.Anther culture conducted before middle of July in Xinjiang was effective.In this study,56 haploid plants were achieved by acclimatization and transplant followed embryo development.

参考文献/References:

[1]王玉英,孙敬三,王敬驹,等.小黑麦和辣椒花粉植株的诱导率[J].中国科学,1973(1):104-107.

[2]GEORGE L,NARAYANASWAMY S.Haploid capsicum through experimental androgenesis[J].Protoplasm,1973,78：467-470.
[3]张馨宇,王永成,刘爱群,等.辣椒花药培养研究新进展[J].中国农学通报,2007(8):331-334.
[4]李春玲,蒋钟仁.甜辣椒花药培养单倍体育种技术[C]//邹学校.中国辣椒.北京:中国农业出版社,2000:191-205.
[5]KRISTIANSEN K，ANDERSON S B．Effects of donor plant temperature，photoperiod and age on anther culture response of Capsicum anuum L.[J].Euphytica,1993,67:105-109.
[6]张树根,沈火林.辣椒花药培养单倍体育种技术研究进展[J].辣椒杂志,2006(3):1-5.
[7]MITYKO J,ANDRASFALVY A,CSILLERY G,et al.Anther-culture response in different genotypes and F1 hybrids of pepper (Capsicum annuum L.)[J].Plant Breeding,1995,114:78-80.
[8]DUMAS der V R,CHAMBONNET D,POCHARD E.Culture in vitro d′antheres de piment (Capsicum annuum L.)：Amelioration destauxd′ obtention de plantes ches differents genotypes par des traitmentsa+35℃[J].Agronomie,1981(1):859-864.
[9]王玉英,李春玲,蒋钟仁.辣椒和甜椒花药培养的新进展[J].园艺学报,1981,8(2):41-45.
[10]张恩让,须海丽.贵州地方辣椒花药培养体系的初步研究[J].辣椒杂志,2005(4):24-27.
[11]赵激,邹学校.不同培养基对辣椒花药培养的影响[J].湖南农业大学学报(自然科学版),2010(4):181-187.
[12]王仲慧,刘金兵,刁卫平,等.辣椒花药培养胚状体发生途径影响因子研究[J].核农学报,2015(8)：1471-1478.
[13]付文婷,廖芳芳,何建文,等.不同温度和外源添加物对辣椒花药的培养效果[J].南方农业学报,2015(4)：635-640.

相似文献/References:

[1]胡凤霞,唐艳领,刘金,等.辣椒砧木的筛选及其耐盐性研究[J].北方园艺,2013,37(04):24.
[2]陈 潇,盛萍萍,刘润进,等.西瓜与辣椒间作体系接种AM真菌对连作西瓜植株[J].北方园艺,2014,38(12):6.
　CHEN Xiao,SHENG Ping-ping,LIU Run-jin,et al.Effect of Intercropping System of Pepper-Watermelon Crops Inoculating Arbuscular Mycorrhizal Fungus on Growth and Defence Enzymes Activity of the Continuous Cropping Watermelon[J].Northern Horticulture,2014,38(19):6.
[3]张婷玉,林 多,杨延杰.辣椒根系分泌物的收集方法研究[J].北方园艺,2014,38(12):14.
　ZHANG Ting-yu,LIN Duo,YANG Yan-jie.Study on Collection Method of Root Exudates on Pepper[J].Northern Horticulture,2014,38(19):14.
[4]叶 林,李春江,张光弟,等.不同育苗穴数、基质配比和施肥量对温室辣椒幼苗生长及其秧苗质量的影响[J].北方园艺,2014,38(13):50.
　YE Lin,LI Chun-jiang,ZHANG Guang-di,et al.Effects of Different Seedling Number of Holes，Substrates and Fertilization on the Growth and Quality of Greenhouse Pepper Seedlings[J].Northern Horticulture,2014,38(19):50.
[5]孔小平,苗增建.等离子体处理辣椒种子对其性状及产量的影响[J].北方园艺,2013,37(13):34.
　KONG Xiao-ping,MIAO Zeng-jian.Effect of Plasma Treatment on Characters and Output of Pepper Seeds[J].Northern Horticulture,2013,37(19):34.
[6]高万里.白城地区辣椒产业发展对策研究[J].北方园艺,2014,38(04):159.
　GAO Wan-li.Study on the Development Countermeasure of Pepper Industry in Baicheng Area[J].Northern Horticulture,2014,38(19):159.
[7]岳振平.不同种植密度对早春大棚辣椒产量和产值的影响[J].北方园艺,2014,38(05):51.
　YUE Zhen-ping.Effect of Different Planting Densty on Fruit Yield and Output Value of Pepper in Early Spring[J].Northern Horticulture,2014,38(19):51.
[8]郭亚华,谢立波,王雪,等.辣椒经地面模拟诱变后的RAPD鉴定[J].北方园艺,2014,38(05):95.
　GUO Ya-hua,XIE Li-bo,WANG Xue,et al.The RAPD Detection of Capsicum annuum L. After Ground Simulation[J].Northern Horticulture,2014,38(19):95.
[9]杨飞,高艳明,李建设.宁夏设施沙培辣椒引种试验[J].北方园艺,2013,37(10):44.
　YANG Fei,GAO Yan-ming,LI Jian-she.Introduction Test of Sand Culture Facilities Pepper in Ningxia[J].Northern Horticulture,2013,37(19):44.
[10]马寿宾,孙艳,王琛,等.分子标记辅助选择辣椒抗疫病新种质研究[J].北方园艺,2013,37(10):107.
　MA Shou-bin,SUN Yan,WANG Chen,et al.Molecular Marker Assisted Selection of Pepper Materials with Resistance to Phytophthora Blight[J].Northern Horticulture,2013,37(19):107.

备注/Memo

第一作者简介：赖黎丽（1983-），女，硕士，农艺师，现主要从事园艺植物生理等研究工作。E-mail：58859598@qq.com.

责任作者：朱华国（1979-），男，博士，副教授，现主要从事植物生物技术等研究工作。E-mail：zhhg_agr@shzu.edu.cn.
基金项目：兵团人才发展博士后专项资助项目（CZ0014）。
收稿日期：2017-07-18

更新日期/Last Update: 2017-10-11