ZHI Yajing,WANG Wenhe,LENG Pingsheng,et al.Components and Content of Flower Pigments in the Petals of Different Color Lilium Species[J].Northern Horticulture,2017,41(09):62-69.[doi:10.11937/bfyy.201709014]
不同花色百合色素种类和含量分析
- Title:
- Components and Content of Flower Pigments in the Petals of Different Color Lilium Species
- Keywords:
- Lilium brownie; flower pigments; component; content
- 文献标志码:
- A
- 摘要:
- 以4个杂种系共20种不同花色的百合品种为试材，采用花色描述、显色反应和紫外可见光谱分析法，研究了不同花色中色素种类的差异；采用GB/12291-90法、pH示差法、NaNO2-AlCl3比色法，研究了不同花色中类胡萝卜素、花青素和类黄酮含量的差异，以期为百合花色素的分离鉴定和培育新花色百合新品种提供参考。结果表明：不同色系的花色素种类差异明显，黄色系和橙色系主要含有类胡萝卜素和黄酮类化合物；红色系主要含花青素和黄酮类化合物，但部分品种仅含有类胡萝卜素；白色系中仅含有黄酮类化合物。相同色系间花色素含量存在差异，“热辣”（‘Batistero’）中花青素含量最高，“黑人蒙特”（‘Monte Negro’）中类胡萝卜素含量最高，“白天堂”（‘White Heaven’）中类黄酮含量最高。
- Abstract:
- The petals of 20 Lilium cultivars belonging to four strains were used as tested materials.The color description，chromogenic reaction and UV-visible spectra of flower pigments were performed to research the imparity of pigment types in different Lilium species.The contents of carotenoids，anthocyanins and flavonoids were determined by GB/12291-90 method，pH-differential spectrophotometry and NaNO2-AlCl3 method.All of those were studied in order to provide a theoretical reference for the separation and identification of lily pigments and the cultivation of new varieties of lily.The results showed that there were significant differences in the pigments types of lily flower.The pigments of yellow and orange petals were carotenoids and flavonoids；the pigments of red petals were anthocyanins and flavonoids，some species only contained carotenoids.There was only flavonoids in the pigments of white petals.There were significant differences in contents of the flower pigments in the same color.‘Batistero’ was the highest content of anthocyanins，the content of carotenoids in ‘Monte Negro’ was the highest，and the content of flavonoids in ‘White Heaven’ was the highest.
参考文献/References:
[1]陈俊愉.中国花卉品种分类学[M].北京:中国林业出版社,2001:248-252. [2]程金水,刘青林.园林植物育种学[M].北京:中国林业出版社,2010:64-75. [3]刘志祥,洪亚辉,莫爱华,等.观赏植物花色分子遗传学及基因工程研究进展[J].湖南农业大学学报(自然科学版),2002,28(6):531-534. [4]王秀刚.百合种质资源的花色评价及形成分析[D].哈尔滨:东北农业大学,2012. [5]MATTHEWS V.The International lily register & checklist (2007)(Fourth Supplement)[M].London:The Royal Horticultural Society,2014:2-3. [6]赵昶灵,郭维明,陈俊愉.梅花花色色素种类和含量的初步研究[J].北京林业大学学报,2004,26(2):84-89. [7]白新祥,胡可,戴思兰,等.不同花色菊花品种花色素成分的初步分析[J].北京林业大学学报,2006,28(5):68-73. [8]安田齐.花色的生理生物化学[M].北京:中国林业出版社,1989:15-54. [9]夏婷,耿兴敏,罗凤霞.不同花色野生百合色素成分分析[J].东北林业大学学报,2013,41(5):108-165. [10]高锦明.植物化学[M].北京:科学出版社,2002:165-169. [11]孙中武.植物化学[M].哈尔滨:东北林业大学出版社,2000:302-316. [12]谭仁祥.植物成分分析[M].北京:科学出版社,2000:112-133. [13]波钦诺克X H.植物生物化学分析方法[M].邢家海,丁钟荣,译.北京:科学出版社,1981:229-261. [14]林文华,郭红辉.植物花色苷[M].北京:科学出版社,2009:87-99. [15]马卡姆K R.黄酮类化合物结构鉴定技术[M].张宝琛,唐崇实,译.北京:科学出版社,1990:43-45. [16]蔬菜汁类胡萝卜素全量的测定:GB/12291-90水果[S].1990. [17]LEE J,DURST R,WROLSTAD R E.Determination of total monomeric anthocyanin pigment content of fruit juices，beverages，natural colorants，and wines by the pH differential method：collaborative study[J].Journal of AOAC International,2005,88(5):1269-12778. [18]张志良.植物生理学实验指导[M].北京:高等教育出版社,2009:135-137. [19]葛雨萱,王亮生,徐彦军.腊梅的花色和花色素组成及其在开花过程中的变化[J].园艺学报,2008,35(9):1331-1338. [20]张剑亮.观赏向日葵花色形成的机理研究[D].福州:福建农林大学,2008. [21]何小玲,王金发.观赏花卉的品质基因及其基因工程问题[J].植物生理学通讯,1998,34(6):462-466. [22]TANAKA Y,TSUDA S,KUSUMI T.Metabolic engineering to modify flower color[J].Plant Cell Physiol,1998,39(11):1119-1126. [23]BANBA H.Pigments of lily flower:ⅠSurvery of anthocyanin[J].Jpn Sci Hort Sci,1967,36(4):433-437. [24]BANBA H.Pigments of lily flower:ⅡSurvery of carotenoid[J].Jpn Sci Hort Sci,1968,37(4):72-82.(in Japanese). [25]郭鸿飞,张延龙,牛立新,等.8种中国野生百合花色素成分分析[J].西北农林大学报,2015,43(3):98-104. [26]NORBAEK R,KONDO T.Anthocyanins from flowers of Lilium ‘Liliaceae’[J].Phytochemistry,1999,50(9):1181-1184. [27]VALADON L R G,MUMMERY R S.Carotenoids of lilies and of red pepper：Biogenesis of capsanthin and capsorubin[J].Z.Pflanzenphysiol(Bd),1977,82(5):407-416. [28]JEKNIC Z,MORRE J T,JEVREMOVIC S,et al.Cloning and functional characterization of a gene for capsanthin synthase from tiger lily (Lilium lancifolium Thunb.‘Splendens’)[J].Plant and Cell Physiology,2012,53(11):1899-1912. [29]DELI J,MOLNDR P,MATUSZ,et al.Isolation and characterization of 3,5,6-trihydroxy-carotenoids from petal of Lilium trigrinum[J].Chromatographia,1998,48(1):27-31. [30]YAMAGISHI M,KISHIMOTO S,NAKAYAMA M.Carotenoid composition and changes in expression of carotenoid biosynthetic genes in tepals of Asiatic hybrid lily[J].Plant Breeding,2010a,129(1):100-107. [31]NAKANO M,NAKATSUKA A,NAKAYAMA M,et al.Mapping of quantitative trait loci for carotenoid pigmentation in flower tepals of Asiatic hybrid lily[J].Scientia Horticulturae,2005,104:57-64. [32]YAMAGISHI M,YOSHIDA Y,NAKAYAMA M.The transcription factor LhMYB12 determines anthocyanin pigmentation in the tepals of Asiatic hybrid lilies(Lilium spp.) and regulates pigment quantity[J].Molecular Breeding,2012,30(2):913-925.
相似文献/References:
[1]朱莉,李云飞,王忠义,等.秸秆发酵技术在百合生产中的应用[J].北方园艺,2013,37(04):74.
[2]闻月冰,邹 宇,胡立明,等.紫甘蓝色素提取条件优化及其稳定性研究[J].北方园艺,2014,38(10):127.
WEN Yue-bing,ZOU Yu,HU Li-ming,et al.Optimized Extraction Conditions and Stability of Purple Cabbage Pigment[J].Northern Horticulture,2014,38(09):127.
[3]王丽媛,周厚高.不同营养组合和施肥量对切花百合光合特性及干物质含量的影响[J].北方园艺,2014,38(04):58.
WANG Li-yuan,ZHOU Hou-gao.Effect of Nutrient Combinations and Fertilizing Amount on the Photosynthetic Characteristics and Dry Matter of Cut-flower Lily[J].Northern Horticulture,2014,38(09):58.
[4]闫京艳,唐道城,巨秀婷,等.焦锑酸钾沉淀法在百合花柱钙分布研究中的应用[J].北方园艺,2014,38(01):78.
YAN Jing-yan,TANG Dao-cheng,JU Xiu-ting,et al.Application of Potassium Pyroantimonate Precipitation on Calcium Distribution in the Style of Lily[J].Northern Horticulture,2014,38(09):78.
[5]赵兴华,吴海红,杨佳明.百合远缘杂交未成熟子房的离体培养研究[J].北方园艺,2014,38(04):90.
ZHAO Xing-hua,WU Hai-hong,YANG Jia-ming.Study on Lily Distant Hybridization in vitroCulture of Immature Ovary[J].Northern Horticulture,2014,38(09):90.
[6]吴 超,丁晓瑜,黎 侠,等.东方百合“Siberia”基因组DNA提取方法比较研究[J].北方园艺,2014,38(02):108.
WU Chao,DING Xiao-yu,LI Xia,et al.Comparative Study on Genomic DNA Extraction Methods From the Oriental Lily ‘Siberia’[J].Northern Horticulture,2014,38(09):108.
[7]张永福,吕金富,韩丽,等.凤仙花属不同花色株系间色素及营养物质含量的差异性研究[J].北方园艺,2013,37(14):77.
ZHANG Yong-fu,LV Jin-fu,HAN Li,et al.Study on Differences of Pigment and Nutrient Substance Among Different Flower’s Colour Strains of Impatiens L.[J].Northern Horticulture,2013,37(09):77.
[8]周春华,尤超,陈凝华.百合组织培养研究进展[J].北方园艺,2013,37(14):193.
ZHOU Chun-hua,YOU Chao,CHEN Ning-hua.Research Advances on Tissue Culture of Lilium brownie[J].Northern Horticulture,2013,37(09):193.
[9]夏九成,郑毅,吕会,等.利用百合花器官诱导试管苗快繁技术研究[J].北方园艺,2013,37(01):102.
XIA Jiu-cheng,ZHENG Yi,LV Hui,et al.The Rapid Propagation of Lily by Inducing Seedling in Test Tube with Floral Organ[J].Northern Horticulture,2013,37(09):102.
[10]时婷婷,张桂玲,温四民,等.百合花粉超低温保存方法研究[J].北方园艺,2013,37(03):64.
SHI Ting-ting,ZHANG Gui-ling,WEN Si-min,et al.Study on Crypreservation Methods of Lilium spp Pollens[J].Northern Horticulture,2013,37(09):64.
备注/Memo
第一作者简介：智雅静（1990-），女，河北石家庄人，硕士研究生，研究方向为园林植物资源与育种。E-mail：564336573@qq.com.责任作者：王文和（1964-），男，内蒙古丰镇人，博士，教授，研究方向为园林植物资源与育种。E-mail：wwhals@163.com.基金项目：北京市科技提升计划资助项目（PXM2016-014207-000015）；北京市科技计划资助项目（D161100001916003）；北京市属高等学校创新团队建设与教师职业发展计划资助项目（IDHT20150503）；科技创新服务能力建设-协同创新中心-林果业生态环境功能提升协同创新中心资助项目（PXM2017_014207_000043）。