CHEN Xuefeng,WU Xiaojian,WANG Canqin,et al.Identification and Physiological Characteristics of Pathogeny Caused Rusty Spot Disease for Pleurotus ostreatus[J].Northern Horticulture,2017,41(08):132-135.[doi:10.11937/bfyy.201708030]
平菇锈斑病病原菌鉴定及其部分生理特性
- Title:
- Identification and Physiological Characteristics of Pathogeny Caused Rusty Spot Disease for Pleurotus ostreatus
- 文献标志码:
- A
- 摘要:
- 以感染锈斑病的平菇为试材，采用组织分离法、柯赫法则、病原菌16S rDNA序列测定的方法分离并鉴定病原菌；采用测定细菌生物量的方法测定病原菌的适宜生长温度、酸碱度和盐度；采用抑菌圈法检测病原菌对抗生素敏感性，以期为研究病害发生与流行规律以及探索有效防治措施奠定基础。结果表明：锈斑病病原菌鉴定为托拉斯假单胞杆菌Pseudomonas tolaasii；该病原菌适生长的温度为21~30 ℃，适宜生长ｐH为7~8，适宜生长盐度为0.5%~2.5%；该病原菌对红霉素、氨苄青霉素不敏感;对链霉素、四环素、庆大霉素和土霉素的最低抑制浓度分别为312.0、156.0、78.0、78.0 μg?mL-1。
- Abstract:
- Pleurotus ostreatus sporocarp naturally infected rusty spot disease that collected from Nanning，Guangxi was used as test material.The pathogen was isolated by tissue isolation and pathogenicity was tested following Koch′s postulate;identification of the pathogen was conducted by ITS 16S rDNA molecular identification.The suitable growth temperature，pH levels and salinity were detected by testing the bacteria biomass.Sensitivity of antibiotics of the pathogen was detected by Haloes methods.The results showed that the pathogen infected the Pleurotus ostreatus was Pseudomonas tolaasii，and the optimal conditions for its growth were as follows,temperature 21-30 ℃;pH 7-8;salinity 0.5%-2.5%.Streptomycin，tetracycline，gentamicin andterramycin could inhibit growth of pathogen and the MIC was 312.0,156.0,78.0，78.0 μg?mL-1，respectively.The pathogen was not sensitive to erythromycin and ampicillin.
参考文献/References:
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备注/Memo
第一作者简介：陈雪凤（1976-），女，本科，高级农艺师，现主要从事食用菌育种及推广等工作。E-mail:xuefeng767@126.com.责任作者：韦仕岩（1965-），男，硕士，研究员，现主要从事食用菌育种等研究工作。E-mail：weisy0608@163.com.基金项目：广西农业科学院基本科研业务专项资助项目（2015JZ59）；国家食用菌产业技术体系广西创新团队建设专项资金资助项目（nycytxgxcxtd-07-02）。