XUE Zhanjun,LI Hui,GAO Zhikui.Effect of Organochlorine Pesticides on Pollution Characteristic and Chlorophyll a Fluorescence of Muskmelon Fruit Surface[J].Northern Horticulture,2017,41(07):18-22.[doi:10.11937/bfyy.201707004]
有机氯农药对甜瓜果面污染特征和叶绿素荧光参数的影响
- Title:
- Effect of Organochlorine Pesticides on Pollution Characteristic and Chlorophyll a Fluorescence of Muskmelon Fruit Surface
- Keywords:
- muskmelon; organo-chlorine pesticides; comprehensive pollution index; chlorophyll fluorescence
- 文献标志码:
- A
- 摘要:
- 以浅色系厚皮甜瓜品种‘九红瑞’为试材，将有机氯杀菌剂（腐霉利，FML）和杀虫剂（高效氯氟氰菊酯，LJZ）分别配制成1 000倍和2 000倍药液，对开花后第15天的甜瓜果实进行单独喷施处理，在果实污染危害症状显现时，利用叶绿素荧光动力学技术对其污染效应进行分析和评价。结果表明：与对照相比（清水加助剂，CK），甜瓜果实在喷施有机氯农药后的第7天便开始产生明显的污染危害症状，且表现出多位点毒害特征，即不仅破坏原初光化学结构，还降低碳吸收动力学活性，更为重要的是增加光能过量耗散，引起光系统II(PSII)乃至整个光合机构的光抑制甚至光损伤，间接导致甜瓜果实表面组织的热灼伤斑点发生。通过模糊数学的隶属函数法对甜瓜果实受有机氯农药污染的程度进行综合评价，结果发现LJZ污染程度明显高于FML的，且随着喷施浓度的增大而增强。
- Abstract:
- The 1 000 and 2 000 times dilution of fungicides (procymidone,FML) and insecticides (lambda-cyhalothrin,LZJ) of organo-chlorine pesticides were alone applied to spray young muskmelon fruits of ‘Jiuruihong’ at the 15th day after flowering,respectively.The chlorophyll fluorescence parameters were measured by Mini-Imaging-Pam to analysis and evaluate the pollution effects of organo-chlorine pesticides.The results showed that,compared with the control (water plus additives,CK),pollution symptom charactered by scatter and non-uniform spots distribution was observed on muskmelon fruit surface at the 7th day after spraying pesticides.Not only had the primary photochemical apparatus been destroyed,but also dynamic activity of carbon absorption had been reduced.Moreover,the excess energy dissipation produced by increase of Y(NPQ) and Y(NO) could cause photoinhibition or damage of PSⅡ and/or entire photosynthetic apparatus,led to the generation of thermal burning spots on muskmelon fruit surface.The comprehensive pollution index (CPI) calculated by using subordinative fuzzy function was used to evaluate pollution level of different organo-chlorine pesticides on muskmelon fruits.The greater pollution level was recorded in LZJ than FML,and the higher concentration related to LJZ practices attributed to severe pollution level.
参考文献/References:
[1]潜宗伟,唐晓伟,吴震.甜瓜不同品种类型芳香物质和营养品质的比较分析[J].中国农学通报,2009,25(12):165-171. [2]黄远,王伟娟,汪力威,等.调亏灌溉对塑料大棚甜瓜光合特性果实产量和品质的影响[J].华中农业大学学报,2016,35(1):31-35. [3]胡国智,冯炯鑫,张炎,等.不同施氮量对甜瓜养分吸收、分配、利用及产量的影响[J].植物营养与肥料学报,2013,19(3):760-766. [4]王艳艳,焦自高,于贤昌.套袋对厚皮甜瓜品质的影响[J].中国蔬菜,2009(16):61-64. [5]李惠.甜瓜果面斑点的发生与套袋防控效应的研究[D].保定:河北农业大学,2014. [6]STRASSER R J,TSIMILLI-MICHAEL M,SRIVASTAVA A.Analysis of the chlorophyll a fluoresecence transient[M]//Papageorgiou G,Govindjee,eds.Series:Advance in photosynthesis and respiration Vol.19.chlorophyll fluorescence:A signature of photosynthesis.The Netherlands:Kluwer Academic Publishers,2004:321-362. [7]BRACK W,FRANK,H.Chlorophyll a fluorescence:A tool for the investigation of toxic effects in the photosynthetic apparatus[J].Ecotoxicology and Environmental Safety,1998,40:34-41. [8]JOSHI M K,MOHANTY P.Chlorophyll a fluorescence as a probe of heavy metal ion toxicity in plants[M]//Papageorgiou G C,Govindjee,eds.Chlorophyll a fluorescence:a signature of photosynthesis.The Netherlands:Springer,2004:637-661. [9]ESCHER B I,QUAYLE P,MULLER R,et al.Passive sampling of herbicides combined with effect analysis in algae using a novel high-throughput phytotoxicity assay (Maxi-Imaging-PAM)[J].Journal of Environmental Monitoring,2006(8):456-464. [10]姜闯道,高辉远,邹琦,等.链霉素处理对玉米叶片叶绿素荧光参数和叶黄素脱环氧化水平的影响[J].植物生理与分子生物学报,2003,29(3):221-226. [11]CHALIFOUR A,SPEAR P A,BOILY M H,et al.Assessment of toxic effects of pesticide extracts on different green algal species by using chlorophyll a fluorescence[J].Toxicological and Environmental Chemistry,2009,91(7):1315-1329. [12]王梅,高志奎,薛占军.套袋对番茄果实表面光系统Ⅱ光能吸收利用的影响[J].应用生态学报,2010,21(9):2329-2334. [13]段喜涵,高小莹,刘希望.苹果幼果期慎用复配药剂[J].西北园艺,2014(12):38. [14]吴降星,王汉荣,茹水江,等.主要农药对黄瓜的药害研究[J].浙江农业科学,2007(3):336-338. [15]王学梅,崔静英,于蓉.不同化学农药处理对甜瓜商品性的影响[J].北方园艺,2012(10):57-59. [16]李玮娟,高志奎,王梅,等.氧化乐果对不结球白菜光系统 II 的毒理效应[J].应用生态学报,2008,19(10):2317-2321.
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备注/Memo
第一作者简介：薛占军(1983-)，男，内蒙古人，博士，讲师，现主要从事设施蔬菜生理生态及生长调控等研究工作。E-mail：xzj_0117@126.com.责任作者：高志奎(1963-)，男，博士，教授，现主要从事设施蔬菜生理生态及生长调控等研究工作。E-mail：gaozhikui2005@163.com.基金项目：河北省科技支撑计划资助项目(13226419D)。