SONG Ying,LIU Junjie,LIU Yanyan,et al.ISSR Analysis of Genetic Diversity of Main Cultivated Strains of Lentinula edodes in Liaoning[J].Northern Horticulture,2017,41(05):82-85.[doi:10.11937/bfyy.201705019]
辽宁省主栽香菇菌株ISSR遗传差异性分析
- Title:
- ISSR Analysis of Genetic Diversity of Main Cultivated Strains of Lentinula edodes in Liaoning
- Keywords:
- Lentinula edodes; ISSR-PCR; clustering analysis
- 文献标志码:
- A
- 摘要:
- 以20个辽宁省主栽香菇菌株为试材，采用ISSR分子标记方法，研究了20个菌株的遗传差异性，并采用NTSYS-PCR软件进行聚类分析。结果表明：从30个随机引物中筛选出9个ISSR备用引物，并用这9个引物对供试香菇菌株基因组DNA进行了ISSR-PCR扩增，共扩增出72条清晰条带，其中多态性条带占83.3%。通过聚类分析发现，异化系数在0.36为阈值时，20个菌株聚为4个组群。
- Abstract:
- Twenty Lentinula edodes strains mainly cultivated in Liaoning were used as test materials，the genetic diversity and cluster analysis were studied by ISSR technique and NTSYS-PCR software，respectively.The results showed that 9 ISSR primers were selected from 30 random primers and 72 clear bands were identified by the ISSR-PCR amplification including 83.3% polymorphic bands.Clustering analysis found that 20 strains were gathered into four groups when the dissimilarity coefficient was 0.36.
参考文献/References:
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备注/Memo
第一作者简介：宋莹(1982-)，女，硕士，助理研究员，研究方向为食用菌遗传育种。E-mail:sy.512@163.com.基金项目：辽宁省农业领域青年科技创新人才培养资助项目（2015026）。