SUN Lili,MU Yunhui,ZHEN Canfu,et al.Genetic Diversity of SSR Analysis of Plum and Apricot in Cold Region[J].Northern Horticulture,2017,41(13):50-55.[doi:10.11937/bfyy.20170286]
寒地李和杏遗传多样性SSR分析
- Title:
- Genetic Diversity of SSR Analysis of Plum and Apricot in Cold Region
- Keywords:
- plum(Prunus salicina L.); apricot(Prunus armeniaca L.); SSR; genetic diversity; clustering analysis
- 文献标志码:
- A
- 摘要:
- 以27份适宜北方生长的李、杏品种为试材，采用SSR分子标记技术对其进行遗传多样性分析，并运用UPGMA聚类分析法，分析了27份试材的亲缘关系，为寒地李、杏种质资源保存和品种选育提供分子生物学依据。结果表明：筛选的25对SSR多态性引物共扩增等位位点245个，其中多态性位点240个，多态性比率为97.96%。每对引物的等位基因数量为4~13，平均值为9.8。有效等位基因数平均值为2.740 5，Shannon′s信息指数平均值为1.052 0，多态性信息含量平均值为0.595 5，表明试材具有较高的遗传多样性。聚类分析结果表明，在遗传相似系数0.612处，27份试材可以分为李和杏两大类群。
- Abstract:
- To explore the genetic diversity of Prunus salicina L.and Prunus armeniaca L.,the genetic diversity of 27 plum and apricot varieties grown in the north were analyzed by using SSR molecular marker technology,and the genetic relationship were analyzed by using UPGMA clustering analysis method in this study in order to provide molecular biological basis for the preservation and breeding of germplasm resources.The results showed that,245 alleles were amplified by 25 pairs of SSR primers and including 240 polymorphic loci,the ratio of polymorphism was 97.96%,4-13 alleles were amplified by per pairs of primer,with a mean of 9.8.The average of effective alleles was 2.740 5,the average of Shannon′s information index was 1.052 0,the average of polymorphism information content was 0.595 5,illustrated the germplasms with high genetic diversity.The clustering analysis results showed that,when the genetic correlation coefficient was 0.612,the 27 resources could be divided into two big groups of plum and apricot.
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备注/Memo
第一作者简介：孙莉莉（1983-），女，博士，助理研究员，研究方向为寒地李杏栽培与分子遗传育种。E-mail:sunlilineau@163.com.责任作者：牟蕴慧（1965-），女，本科，研究员，研究方向为寒地核果类果树育种与栽培技术。E-mail:yunhuimu@163.com.基金项目：“十二五”农村领域国家科技计划课题资助项目（2013BAD02B03）；哈尔滨市科技局资助项目（2016RAXYJ011）。