GAO Guifeng,WANG Junling,CHU Haijiao.Extraction of Atrazine-degrading Enzymes and Enzyme Activity Analysis[J].Northern Horticulture,2016,40(14):94-96.[doi:10.11937/bfyy.201614023]
阿特拉津降解酶的提取及酶活性分析
- Title:
- Extraction of Atrazine-degrading Enzymes and Enzyme Activity Analysis
- Keywords:
- atrazine-degrading enzymes; HPLC; degradability
- 文献标志码:
- A
- 摘要:
- 以阿特拉津降解菌株为试验材料，采用冰浴超声破壁水提法提取粗酶，用氯仿萃取培养液里残余的阿特拉津，用HPLC测定阿特拉津的残留量，在温度、pH、粗酶液含量不同、培养时间不同的条件下，研究了粗酶对阿特拉津降解率的影响，以期更好地利用阿特拉津降解酶。结果表明：阿特拉津降解酶最适宜的温度为35 ℃左右，最适pH 7.5；当粗酶液含量达到50%以上时，底物降解比较完全。
- Abstract:
- Taking atrazine degradation strains as experimental material.Crude enzyme was extracted by the ultrasonic wall-breaking water methods.Residual atrazine was extracted with chloroform in the culture medium.The residues of atrazine was measured by HPLC.Under different conditions of temperature，pH，crude enzyme fluid content，training time，crude enzyme was studied on the influence of atrazine degradation rate,in order to make better use of atrazine-degrading enzymes.The results showed that atrazine degradation enzyme of optimum temperature was about 35 ℃.The optimal pH was 7.5.When the crude enzyme liquid content was above 50%，the substrate degradation was complete.
参考文献/References:
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备注/Memo
第一作者简介：高桂凤（1971-），女，吉林吉林人，硕士，讲师，现主要从事微生物等研究工作。E-mail：791979258@qq.com.基金项目：吉林省教育厅“十二五”科学技术研究资助项目（吉教科合字[2014]第391号)；吉林农业科技学院微生物重点学科培育资助项目（吉农院合字[2013]第X010号）。