LIN Xiaoping,LAI Zhongxiong.Effect of Different Light Qualities on Photosynthetic Characteristics and Polysaccharide Accumulation of Dendrobium officinale[J].Northern Horticulture,2016,40(13):154-158.[doi:10.11937/bfyy.201613040]
不同光质对铁皮石斛光合特性和多糖积累的影响
- Title:
- Effect of Different Light Qualities on Photosynthetic Characteristics and Polysaccharide Accumulation of Dendrobium officinale
- Keywords:
- light quality; Dendrobium officinale; chloroplast number; chlorophyll content; polysaccharide content
- 文献标志码:
- A
- 摘要:
- 以铁皮石斛试管苗为试材，研究了不同光质对其光合特性和多糖积累的差异。结果表明：不同光质条件下，试管苗叶中叶绿体数量及叶绿素含量有明显差异，二者呈负相关；白光和绿光下多糖含量和叶绿素含量变化趋势基本一致，表现为白光>绿光；在红、蓝光下则表现出低叶绿素含量、高多糖含量的特征，表现出光质对光合代谢调控的复杂性。
- Abstract:
- The in vitro shoots of Dendrobium officinale were chosen as materials to study the effects of different light qualities on photosynthetic characteristics and the differences inpolysaccharide accumulation.The results showed that,under the condition of different light qualities,there existed in leaves of in vitro shoots significant difference between chloroplast number and chlorophyll content,which were negatively correlated；the change trend of polysaccharide content and chlorophyll content were basically the same under white light and green light,exhibiting white>green,and it was characterized by low chlorophyll content and high polysaccharide content under red light and blue light,showing the complexity of photosynthetic metabolism regulation under different light qualities.
参考文献/References:
[1]刘向东.利用激光扫描共聚焦显微镜研究植物细胞发育形态学变化[J].激光生物学报,2007,16(2):173-178. [2]刘秀丽,宋平,孙成明.植物叶绿素测定方法的再探讨[J].江苏农业研究,1999,20(3):46-67. [3]李志丹,韩瑞宏,廖桂蓝,等.植物叶片中叶绿素提取方法的比较研究[J].广东第二师范学院学报,2011,3(31):80-83. [4]李彩霞,竹剑平.不同采收期铁皮石斛中多糖含量比较[J].药物分析杂志,2010,30(6):1138-1149. [5]高亭亭,斯金平,朱玉球,等.光质与种质对铁皮石斛种苗生长和有效成分的影响[J].中国中药杂志社,2012,37(2):198-201. [6]TANA K A,TAKAMU R A,WATANA B E,et a1.In vitro growth of cymbidium plantlets cultured under superbright red and blue light-emitting diodes(leds)[J].Journal of Horticultural Science & Biotechnology,1998,73:39-44. [7]ANZEL K A,RENA T A,SILV A,et al.In vitro cultivation of grape culture under solid-state lighting[J].Scientific Works of the Lithuanian Institute of Horticulture and Lithuanian University of Agriculture,2007,26(3):235-245. [8]徐凯,郭延平,张上隆.不同光质对草莓叶片光合作用和叶绿素荧光的影响[J].中国农业科学,2005,38(2):369-375. [9]江明艳.遮荫和光质对一品红生长发育的影响研究[D].雅安:四川农业大学,2004. [10]ANDERSON,JAN M.Photoregulation of the composition,function and structure of thylakoid membranes[J].Plant Physio,1986,37(1）:9-136. [11]迟伟,王荣富,张成林.遮荫条件下草莓的光合特性变化[J].应用生态学报,2001,12(4):566-568. [12]RIVKIN R B.Influence of irradiance and spectral quality on the carbon metabolism of phytoplankton in photo-synthesis,chemical composition and growth[J].Marine Ecology Progress Series,1989,55:291-304. [13]车生泉.光质对小苍蓝茎尖试管培养的影响[J].园艺学报,1997,24(3):269-273. [14]储钟稀,童哲,冯丽洁,等.不同光质对黄瓜叶片光合特性的影响[J].植物学报,1999,41(8):867-870. [15]杜洪涛,刘世琦,蒲高斌.光质对彩色甜椒幼苗生长及叶绿素荧光特性的影响[J].西北农业学报,2005,14(1):41-45. [16]刘立侠,唐树延,许守民,等.光质对人参叶绿体结构和光合生理性状的影响[J].植物学报,1993,35(8):588-592. [17]李韶山,潘瑞炽.蓝光对水稻幼苗碳水化合物和蛋白质代谢的调节[J].植物生理学报,1995,21(1):22-28． [18]张玲菊,高亭亭,章晓玲,等.5 个种源铁皮石斛的光合特性[J].浙江农林大学学报,2013,30(3):359-363. [19]王振兴,艾军,陈丽,等.软枣猕猴桃光合日变化的研究[J].北方园艺,2010(6):29-31. [20]刘汉峰.CO2倍增对三种石斛光合特性的影响[D].北京:北京林业大学,2012. [21]孙志蓉,王美云,张宏桂,等.环草石斛和铁皮石斛试管苗叶片气孔特征比较[J].安徽农业科学,2011,39(27):16583-16586. [22]濮晓珍，尹春英，周晓波，等.铁皮石斛组培苗移栽驯化过程中叶片光合特性、超微结构及根系活力的变化[J].生态学报,2012,32(13):4114-4122. [23]李雪,刘建福,曾小爱,等.不同基质和容器对铁皮石斛生长和生理特性的影响[J].西南师范大学学报（自然科学版),2012,37(8):124-128. [24]SAEBO A,KREKLING T,APPELGREN M.Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro[J].Plant Cell,Tissue and Organ Culture,1995,41:177-185. [25]VLASOVA M P,DROZDOVA S,VOSKRESENSKAYA N P.Modification of chloroplast ultrastructure in pea plants greening under blue and red light[J].Fiziol Rast(USSR),1971,18:5-11. [26]史宏志,韩锦峰,张国显,等.单色蓝光和红光对烟苗叶片生长和碳氮代谢的影响[J].河南农业大学学报,1998(3):258-262． [27]戴绍军,王洋,阎秀峰,等.滤光膜对喜树幼苗叶片生长和喜树碱含量的影响[J].生态学报,2004,24(5):869-875. [28]徐克章,曹正菊,张为群,等.人参叶片光合作用特性的研究[J].中国农业科学,1990(23):69-74. [29]ARNON,D I.Copper enzymes in isolated chloroplasts.Polybhenoloxidase in Beta vulgais[J].Plant Physiol,1949,24(1):1-15. [30]KASPERBAUER M J.Strawberry yield over red versus black plastic much[J].Crop Sci,2000,40:171-174.
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备注/Memo
第一作者简介：林小苹（1975-），女，硕士，副教授，研究方向为植物生理与生物技术。E-mail：lxp103@126.com.责任作者：赖钟雄（1966-），男，博士，教授，研究方向为植物生理与生物技术。E-mail：laizx01@163.com.基金项目：漳州市自然基金资助项目（zz2013J12）；福建省农业科技平台建设资助项目（2008N2001）。