XUE Mei,LI Xiaoying,ZHOU Weiping.Study on Extraction Technology and Antioxidant Property of Total Flavonoids From Lilium brownii var.viridulum Leaves[J].Northern Horticulture,2016,40(11):127-130.[doi:10.11937/bfyy.201611033]
龙牙百合叶总黄酮提取工艺及抗氧化性研究
- Title:
- Study on Extraction Technology and Antioxidant Property of Total Flavonoids From Lilium brownii var.viridulum Leaves
- Keywords:
- Lilium brownii var.viridulum leaves; total flavonoid; ultrasonic method; antioxidant activity
- 文献标志码:
- A
- 摘要:
- 以龙牙百合叶片为研究对象，采用优化超声波法提取龙牙百合叶片总黄酮，对黄酮进行了抗氧化活性分析，并在单因素试验的基础上采用正交实验法，研究了乙醇浓度、料液比、提取时间、提取温度对总黄酮提取效果的影响，并确定了最佳工艺条件，对其体外抗氧化活性进行了初步研究。结果表明：最佳工艺条件为提取温度50 ℃，乙醇浓度80%，料液比1∶5 g?mL-1，提取时间30 min。此条件下，百合叶总黄酮含量为12.691 mg?g-1。抗氧化试验结果表明，龙牙百合叶总黄酮提取液对DPPH?的清除效果优于相同浓度下维生素C溶液，并呈显著差异。
- Abstract:
- Extraction technology by ultrasonic method and antioxidant activity of total flavonoids from Lilium brownii var.viridulum leaves were studied.Basing on the single factor experiment，orthogonal experiment was used to study the effect of ethanol concentration，material-liquid ratio，extraction time and extraction temperature on the quantity of flavonoids extracting from Lilium brownii var.viridulum leaves.Antioxidant activity of flavonoids extraction in vitro was researched preliminarily.The results showed that the optimum extracting conditions of total flavonoids from Lilium brownii var.viridulum leaves were extraction temperature 50 ℃，ethanol concentration 80%，solid-liquid ratio 1∶5 g?mL-1，extraction time 30 minutes，the total flavonoids content was 12.691 mg?g-1.The results of antioxidant test showed that the DPPH? eliminating ability of total flavonoids extraction from Lilium brownii var.viridulum leaves was better than the same concentration of vitamin C，and showed a very significant difference.
参考文献/References:
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备注/Memo
第一作者简介：薛梅（1973-），女，硕士，实验师，现主要从事植物生理及植物种质资源开发与利用等研究工作。E-mail:xuemei.1998@163.com.责任作者：李晓英（1973-），女，硕士，副教授，现主要从事生物活性物质的提取与检测等研究工作。E-mail：lxyi2@126.com.基金项目：重庆市教委科研资助项目（KJ1401129）。