CHEN Yueying,WANG Yanping,SUN Ruilin,et al.Study on Optimization of Microwave-assisted Extraction of Proanthocyanidins From Grape Residues and Its Antioxidant Activity[J].Northern Horticulture,2016,40(11):123-126.[doi:10.11937/bfyy.201611032]
葡萄皮渣原花青素微波辅助提取工艺的优化及其抗氧化活性研究
- Title:
- Study on Optimization of Microwave-assisted Extraction of Proanthocyanidins From Grape Residues and Its Antioxidant Activity
- 文献标志码:
- A
- 摘要:
- 以葡萄皮渣为试材,采用微波辅助提取方法和体外抗氧化试验,研究了葡萄皮渣中原花青素提取工艺和体外抗氧化活性。结果表明:最佳工艺为乙醇体积分数60%(v/v)、料液比1∶20 g?mL-1、微波时间90 s、微波功率800 W。在此条件下,原花青素提取率为9.12 mg?g-1。体外抗氧化试验中,葡萄皮渣原花青素提取物表现明显的抗氧化能力,对DPPH自由基(DPPH?)和羟基自由基(?OH)具有较好的清除能力,但抗氧化能力比维生素C弱。
- Abstract:
- Taking grape residue as the experimental material,the extraction technology and antioxidant activity of proanthocyanidins from grape residue were studied by microwave assisted extraction and in vitro antioxidant activity.The results showed that the optimum condition were as follows,60%(v/v) ethanol as the solvent,material-solvent ratio 1∶20 g?mL-1,microwave time 90 seconds,microwave power 800 W.Under such conditions,the yield of proanthocyanidins was 9.12 mg?g-1.The extraction from grape residues showed significant antioxidant capacity,and activity to removing DPPH? and ?OH,but weaker than vitamin C.
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备注/Memo
第一作者简介:陈月英(1964-),女,硕士,教授,现主要从事营养与食品安全等研究工作。E-mail:14389487@qq.com.基金项目:河南农业职业学院科学研究资助项目(ky2015-zr-08);2015年度河南省高等学校优秀教学团队建设资助项目;2014年度河南省高等学校“专业综合改革试点”资助项目。