WEI Xin,WEI Yongxiang,LIU Cheng,et al.Mutagenic Effect on Tissue Culture Plants and Nutritional Bowl Plants of Blueberry Induced by 60Co-γ Irradiation[J].Northern Horticulture,2016,40(10):6-9.[doi:10.11937/bfyy.201610002]
60Co-γ辐射对越橘组培苗和营养钵苗的诱变效应
- Title:
- Mutagenic Effect on Tissue Culture Plants and Nutritional Bowl Plants of Blueberry Induced by 60Co-γ Irradiation
- Keywords:
- blueberry; 60Co-γ; mutation effects
- 文献标志码:
- A
- 摘要:
- 以 ‘斯巴坦’及‘蓝丰’越橘组培苗、1年生‘斯巴坦’及‘蓝丰’和‘奥尼尔’越橘营养钵苗为试材，采用7个剂量的60Co-γ射线辐射处理，观察比较不同辐射处理条件下组培苗的生长植株比例、株高和增殖系数及营养钵苗的株高、茎粗和成活率。结果表明：随着60Co-γ辐射剂量的增加，组培苗的生长植株比例、株高和增殖系数呈下降趋势。‘蓝丰’组培苗辐照剂量大于50 Gy时生长受到严重抑制，‘斯巴坦’辐照剂量大于70 Gy时生长受到严重抑制；‘斯巴坦’和‘奥尼尔’株高和茎粗随辐射剂量的增加总体上呈下降趋势，‘蓝丰’茎粗随辐射剂量的增加总体上呈下降趋势，但株高随辐射剂量的增加总体上呈上升趋势。
- Abstract:
- Taking the tissue culture plants of ‘Bluecrop’，‘Spartan’ and the nutritional bowl plants of ‘Bluecrop’，‘Spartan’，‘O’Neal’ trees as materials.Seven 60Co-γ doses were selected to irradiate the growth ratio，plant height，multiplication constant of tissue culture plant and the plant height，stem diameter，survival rate of nutritional bowl plants were investigated．The results showed that with the increment of the 60Co-γ iradiation dose，the growth ratio，plant height，multiplication constant of tissue culture plant were showed a decling trend.The growth of tissue culture plant for ‘Bluecrop’ were inhibited on more than 50 Gy irradiation dose，The growth of tissue culture plant for ‘Spartan’ were inhibited on more than 70 Gy irradiation dose；the plant height and stem diameter of nutritional bowl plants for ‘Spartan’ and ‘O’Neal’ were showed a decling trend.The stem diameter of nutritional bowl plants for ‘Bluecrop’ were showed a decling trend，but the height showed a rising trend.
参考文献/References:
[1]李亚东,刘海广,唐雪东.蓝莓栽培图解手册[M].北京:中国农业出版社,2014.
[2]KADER F，ROVEL B.Fractionation and identification of the phenolic compounds of highbush blueberries(Vaccinium corymbosum L.)[J].Food Chemistry,1996,55(1):35-40.
[3]POONNACHIT U,DARNELL R.Effect of ammonium and nitrate on ferric chelate reductase and nitrate reductase in Vaccinium species[J].Annals of Botany,2004,93(4):399-405.
[4]辛培尧,周军,唐军荣,等.60Co-γ辐射对华山松南方种源种子的诱变效果[J].贵州农业科学,2012,40(9):190-191.
[5]胡德活,韦如萍乐昌含笑种源与家系种子育苗试验[J].广州林业科技,2006,22(3):1-6.
[6]赵剑,李建国.60Co-γ射线辐射诱变提高长春花生物碱含量[J].分子植物育种,2009,7(3):607-611.
[7]洪亚辉,朱兆海,黄璜,等.菊花组织培养与辐射诱变的研究[J].湖南农业大学学报(自然科学版),2003(6):457-461.
[8]范建新,邓仁菊,李金强.果树诱变育种研究进展[J].安徽农业科学,2008,36(22):9455-9457,9598.
[9]廖飞雄,潘瑞炽.60Co-γ辐射对菜心种子萌发和幼苗生长的效应[J].核农学报,2001,15(1):6-10.
[10]杨静坤,黄丽萍,唐敏,等.碧玉兰试管植株辐射诱变初探[J].现代园艺,2008(10):6-9.
[11]张福翠,熊作明,胡艺春,等.大花美人蕉辐射诱变育种研究[J].安徽农业科学，2011,39(1):56-58.
[12]陈发棣,滕年军,房伟民,等.三个菊花品种花器官愈伤组织辐射效应的研究[J].中南林学院学报,2003,23(5):49-52.
[13]王红,陈发棣,房伟民,等.平阳霉素对小菊品种离体培养的影响[J].西北植物学报,2007,27(4):693-698.
[14]邢莉莉,陈发棣,陈素梅.60Co-γ辐射对切花菊试管苗的诱变效应[J].园艺学报,2010,37(7):1117-1124.
[15]王晶,刘录祥,赵荣,等.60Co-γ射线对菊花组培茼的诱变效应[J].农业生物技术学报,2006,14(2):241-244.
[16]DIN R,AHMED Q K,JEHAN S.Studies for days taken to earing initiation and earing completion in M J generation of different wheat genotypes irradiated with various doses of gamma radiation[J].Asian Journal of Plant Sciences,2003(2):894-896.
[17]AKGUN I，TOSUN M.Agricultural and cytological characteristics of M I perennial rye (Secale montanum Guss．)as effeeted by the application of different doses of gamma rays[J].Pakistan Journal of Biological Sciences,2004,7(5):827-833.
[18]DIN R,QASIM M,AHMAD K.Radio sensitivity of various wheat genetypes in M l generation[J].International Journal of Agriculture and Biology,2004,5(6):898-900.
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备注/Memo
第一作者简介：魏鑫（1982-），男，硕士，助理研究员，研究方向为蓝莓种质资源与栽培生理。E-mail：run2010@163.com.责任作者：杨艳敏（1975-），女，本科，副研究员，研究方向为小浆果种质资源。E-mail：yymzcb@163.com.基金项目：科技部科技支撑计划资助项目（2013BAD02B04-04）；辽宁省科技厅农业攻关计划资助项目（2014204007）。