[1]周丽英,卜璐璐,杨春雷,等.番茄PG基因启动子的序列分析[J].北方园艺,2016,40(01):72-75.[doi:10.11937/bfyy.201601019]
　ZHOU Liying,BU Lulu,YANG Chunlei,et al.Cloning and Sequence Analysis of Polygalac-turonase (PG) Promoters in Tomato[J].Northern Horticulture,2016,40(01):72-75.[doi:10.11937/bfyy.201601019]
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番茄PG基因启动子的序列分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷 期数: 2016年01 页码: 72-75 栏目: 出版日期: 2016-01-15
Title:
Cloning and Sequence Analysis of Polygalac-turonase (PG) Promoters in Tomato
作者:
周丽英1; 卜璐璐1; 杨春雷1; 侯思名2; 田甜3; 杨正安1
（1.云南农业大学 园林园艺学院，云南 昆明 650201；2.云南昆明学院 生命科学与技术系，云南 昆明 650213；3.云南省昭通市巧家县农业局农技中心站，云南 昭通 654600）
Author(s):
ZHOU Liying1; BU Lulu1; YANG Chunlei1; HOU Siming2; TIAN Tian3; YANG Zheng’an1
（1.Gardening College,Yunnan Agricultural University，Kunming，Yunnan 650201；2.Department of Lifescience and Technique,College of Kunming,Kunming,Yunnan 650213；3.Extension Center of Agricultural Technology of Qiaojia County，Zhaotong，Yunnan 654600）
关键词:
番茄; PG基因; 启动子
Keywords:
Solanum lycopersicum; polygalac-turonase gene; promoter
DOI:
10.11937/bfyy.201601019
文献标志码:
A
摘要:
以“中蔬四号”番茄为试材，利用PCR技术从“中蔬四号”番茄中扩增出1 500 bp的PG基因启动子，并对启动子序列进行了生物信息学分析。结合PLANTCARE和PLACE数据库Database预测分析PG启动子。结果表明：PG启动子具有多个典型的TATA〖KG-*2〗-Box和CAAT-Box等基本元件，以及光响应元件、热响应元件、乙烯响应元件、植物组织特异调控元件、胚乳表达相关调控元件、逆境胁迫响应元件、生理昼夜节律控制元件等，说明番茄PG基因的表达与光、温度、激素、逆境等因素有关，为以后深入研究PG基因的调控等研究提供理论基础。
Abstract:
Taking tomato variety of‘Zhongshu No.4’as material,1 500 bp PG gene promoter was amplified in tomato leaves using PCR，and bioinformatics analysis of PG promoter sequences was conducted.The results showed that by PLANTCARE database and PLANTCARE database showed that PG gene promoter had number of typical core elements such as TATA-Box，CAAT-Box，photoresponse element，thermal response element，ethylene response element，regulatory element related to tissue specific activation，regulatory element involved in endosperm expression，element involved in defense and stress responsiveness，circadian rhythm controlling element and so on，all of which laid the groundwork for such research on the future regulation of PG genes.

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备注/Memo

第一作者简介：周丽英（1991-），女，山东聊城人，硕士研究生，研究方向为蔬菜生物学及生物技术。E-mail：1153469555@qq.com.责任作者：杨正安（1974-），男，云南昆明人，博士，副教授，现主要从事蔬菜生物技术与遗传育种等研究工作。E-mail：454483788@qq.com.基金项目：云南省自然科学基金资助项目（2011FB049）；国家自然科学基金资助项目（31260481，31460516）。

更新日期/Last Update: 2016-03-02