GAO Qingya,ZHAO Baotang,ZHANG Ji,et al.Optimization of Ultrasound-assisted Extraction of Protein From Nitraria tangutorum Bor by Response Surface Methodology[J].Northern Horticulture,2015,39(14):141-147.[doi:10.11937/bfyy.201514037]
响应面优化超声辅助提取唐古特白刺蛋白质的工艺研究
- Title:
- Optimization of Ultrasound-assisted Extraction of Protein From Nitraria tangutorum Bor by Response Surface Methodology
- 文献标志码:
- A
- 摘要:
- 以唐古特白刺为试材，在单因素试验的基础上，选取液料比、超声时间、超声功率和提取温度为影响因子，应用Box-Behnken中心组合设计建立数学模型，以蛋白质得率为响应值，进行响应面分析。结果表明：超声波提取唐古特白刺中蛋白质的最佳工艺条件为超声时间30 min，超声温度50℃，液料比为20∶1 mL/g，超声功率150 W，提取次数2次，在此条件下唐古特白刺蛋白质得率达到(11.87±0.32)％。响应面法建立的数学模型和试验数据相符，优化的唐古特白刺中蛋白质提取工艺方法简便，为进一步开发唐古特白刺蛋白提供了依据。
- Abstract:
- Taking Nitraria tangutorum Bor as test material，based on single factor experiments，solvent-solid ratio，ultrasonic time，supersonic power and ultrasound tissue temperature were selected as influencing factors.The experiment mathematical model was arranged according to Box-Behnken central composite design,for protein yield as response values.The results showed that the optimum extraction conditions were that the ultrasonic time 30 minutes，ultrasonic temperature 50℃，the ratio of liquid to solid 20∶1 mL/g，ultrasonic power 150 W.The protein extraction yield was (11.87±0.32)％.The mathematical model and the experimental data were consistented with response surface method;the optimization of the fruit of Nitraria tangutorum Bor protein extraction process was simple,provided the basis for the further development of Nitraria tangutorum Bor protein.
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备注/Memo
第一作者简介：高清雅（1988-），女，硕士研究生，研究方向为植物生理与生态。E-mail：gaoqingya30@126.com.责任作者：赵庆芳（1962-），女，教授，现主要从事植物生理学等研究工作。E-mail：1083455950@qq.com.基金项目：国家科技支撑计划资助项目（2012BAD36B04）。