[1]夏光辉,刘 欢,何文兵.双酶法提取角瓜籽仁蛋白质的工艺研究[J].北方园艺,2014,38(02):137-140.
　XIA Guang-hui,LIU Huan,HE Wen-bing.Study on Extraction Technology of Zucchini Seed Protein Using Double Enzyme Hydrolysis[J].Northern Horticulture,2014,38(02):137-140.
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双酶法提取角瓜籽仁蛋白质的工艺研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年02期 页码: 137-140 栏目: 出版日期: 2014-01-31
Title:
Study on Extraction Technology of Zucchini Seed Protein Using Double Enzyme Hydrolysis
文章编号:
1001-0009(2014)02-0137-04
作者:
夏光辉1; 2; 刘 欢1; 何文兵1
1.通化师范学院 长白山食品工程研究中心，吉林 通化 134002；
2.通化师范学院 制药与食品科学学院，吉林 通化 134002
Author(s):
XIA Guang-hui1; 2; LIU Huan1; HE Wen-bing1
1.Research Center of Changbai Mountain Food Engineering，Tonghua Normal University,Tonghua,Jilin 134002；
2.College of Pharmaceutical and Food Science,Tonghua Normal University，Tonghua,Jilin 134002
关键词:
角瓜籽; 蛋白质; 酶; 提取; 工艺
Keywords:
zucchini seed; protein; enzyme; extraction; technology
分类号:
S 642. 6
文献标志码:
A
摘要:
以角瓜籽为试材，采用超声波进行预处理，选取纤维素酶和Alcalase碱性蛋白酶以2∶1比例复配为复合酶制剂，研究了双酶法提取角瓜籽仁蛋白质的最佳工艺条件。结果表明：角瓜籽仁蛋白质的最佳提取工艺为：料液比1∶20 g/mL、总加酶量7%、酶解温度55℃、酶解时间120 min，酶解pH 7. 5，在此条件下角瓜籽仁蛋白质的提取率最高，为74. 2%。
Abstract:
Taking the zucchini seed as material，using ultrasonic to preprepared,the cellulase and alcalase as multi-enzyme with a mixture of cellulose and alcalase at 2∶1 activity ratio was chosed to study the optimal hydrolysis conditions for double enzyme hydrolysis extraction technology of zucchini seed protein.The results showed that the optimum extraction technology were diluted the substrate with water at the ratio of 1∶20 g/mL，added the multi-enzyme with 7% total dose,hydrolyzed the protein at the pH 7. 5 and temperature of 55℃ for 120 min.The protein extraction efficiency of 74. 2% could be observed under these conditions.

参考文献/References:

[1]陈聪颖,陈泽乃.西葫芦的化学和药理研究[J].国外医药(植物药分册),1998(5)：215-217.

[2]刘畅,宋振伟.白瓜籽剥壳分离设备的开发与应用[J].农业机械,2009(16)：103-104.

[3]麻成金,黄伟,黄群,等.复合酶法提取仿栗籽蛋白的工艺优化[J].食品科学,2012,33(20)：34-39.

[4]芮闯,侯彩云,刘莹,等.水酶法提取花生蛋白质的研究[J].粮油食品科技,2009,17(4)：22-25.

[5]方芳,许凯扬,朱强,等.超声波辅助水酶法萃取葫芦籽油的研究[J].中国粮油学报,2012,27(10)：62-66.

[6]杨萌萌,郭兆斌,余群力,等.超声波辅助法提取胶原蛋白工艺研究[J].甘肃农业大学学报,2013,48(3)：121-126.

[7]Zhang S B，Wang Z，Xu S Y.Optimization of aqueous enzymatic extraction of rapeseed oil and protein hydrolysates[J].Journal of the American Oil Chemists Society,2007,84(1)：97-105.

[8]谭志光,唐书泽,汪勇,等.酶法制取早籼米浓缩蛋白[J].食品科学,2006,32(4)：54-57.


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备注/Memo

第一作者简介：夏光辉（1978-），男，吉林通化人，硕士，讲师，现主要从事农产品加工与贮藏等研究工作。E-mail：xiaghui@163.com.

更新日期/Last Update: 2014-08-10