BU Jiaodi,CHEN Yingying,LIU Mengjun,et al.Cloning and Expression Analysis of Glyceraldehyde-3-phosphate Dehydrogenase From Ziziphus jujuba[J].Northern Horticulture,2015,39(13):102-106.[doi:10.11937/bfyy.201513030]
枣甘油醛-3-磷酸脱氢酶基因的克隆及表达分析枣甘油醛-3-磷酸脱氢酶基因的克隆及表达分析
- Title:
- Cloning and Expression Analysis of Glyceraldehyde-3-phosphate Dehydrogenase From Ziziphus jujuba
- 文章编号:
- 1001-0009（2015）13-0102-05
- 关键词:
- 枣; 甘油醛-3-磷酸脱氢酶; 克隆：表达分析
- 分类号:
- Q 786;S 665
- 文献标志码:
- A
- 摘要:
- 以‘金丝小枣’为试材，根据GAPDH基因保守序列设计引物，采用同源克隆和RT-PCR法，克隆枣GAPDH基因并进行生物信息学及转录表达分析。结果表明：该基因包含完整的开放阅读框为1 020 bp，编码339个氨基酸残基，预测分子量为36.904 kDa，理论等电点为8.53，命名为ZjGAPDH（KP147910）；该蛋白包含2个保守结构域，即Gp_dh_N superfamily和Gp_dh_C superfamily；系统进化树分析表明，ZjGAPDH与其它物种GAPDH蛋白的同源性相对较小，但均属于存在细胞质中参与糖酵解过程的蛋白；实时荧光定量分析表明，ZjGAPDH基因在枣果实不同发育时期中表达有显著差异，在幼果期和全红期果实中的表达丰度较高。
- Abstract:
- Taking Ziziphus jujube Mill.‘Jinsixiaozao’as material,a fulllength cDNA sequence of GAPDH gene was cloned from Chinese jujube by homologous gene cloning method and RT-PCR method.The results showed that，the gene was 1 020 bp，the estimated molecular weight and isoelectric point of the putative protein were 36-904 kDa and 8.53，named as ZjGAPDH (accession number KP147910).The protein had two conserved domains named as Gp_dh_N superfamily and Gp_dh_C superfamily.Phylogenic analysis showed that ZjGAPDH had a low homology with that of other species but all belong to cytoplasmic proteins and involve in glycolysis.Qrt-PCR analysis showed that the ZjGAPDH expressions at different riping stages of jujube fruit were significantly different，and the expression level of ZjGAPDH at young fruit stage and whole-red ripening stage were higher than that of other stages.
参考文献/References:
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备注/Memo
第一作者简介：卜娇迪（1990-），女，辽宁铁岭人，硕士研究生，研究方向为细胞工程。E-mail：bujiaodi713138@163.com.责任作者：赵锦（1977-），女，河北安平人，博士，教授，研究方向为植物资源评价。E-mail：zhaojinbd@126.com.基金项目：国家科技支撑计划资助项目（2013BAD14B03）；河北省科技支撑计划资助项目（11230606D-6）。