WU Qing-qing,DOU Yun,ZHANG Chao-jun,et al.Research on Rapid Propagation Technology of Two Lily Commercial Varieties[J].Northern Horticulture,2015,39(12):96-99.[doi:10.11937/bfyy.201512025]
两个百合商业品种的组培快繁技术研究
- Title:
- Research on Rapid Propagation Technology of Two Lily Commercial Varieties
- 文章编号:
- 1001-0009（2015）12-0096-04
- Keywords:
- lily; explant; tissue culture
- 分类号:
- S 682.2+65
- 文献标志码:
- A
- 摘要:
- 以东方百合商业品种‘Sorbonne’、‘Corvara’的球茎鳞片为试材，采用MS培养基为基础培养基，研究不同浓度的植物生长调节剂及蔗糖对百合组培苗生长的影响，筛选最合适配方。结果表明：最适外植体灭菌时间为10 min。‘Sorbonne’ 最佳不定芽诱导培养基配方为MS+30 g/L蔗糖+0.8%琼脂+6-BA 0.8 mg/L+NAA 0.1 g/L，增殖培养基配方为MS+30 g/L蔗糖+0.8%琼脂+6-BA 0.8 mg/L。‘Corvara’ 最佳不定芽诱导配方为MS+30 g/L蔗糖+0.8%琼脂+6-BA 0.2 mg/L+NAA 0.1 g/L，增殖配方为MS+30 g/L蔗糖+0.8%琼脂+6-BA 0.6 mg/L，使用高浓度蔗糖代替激素进行增殖，最佳培养基配方为MS+蔗糖60 g/L+0.8%琼脂，最佳生根培养基配方为MS+30 g/L蔗糖+0.8%琼脂+NAA 0.1 g/L。
- Abstract:
- Taking the bulb scales of lily commercial oriental varieties of ‘Sorbonne’and‘Corvara’as the test materials and MS medium as the minimal medium，the experimental effect and screen the optimal formula with addition of plant growth regulator and sucrose of different concentrations was compared to lily tissne cultured seedling.The results showed that the optimal sterilizing time of explant was 10 min.The optimal formula for the inducing medium of adventitious buds of ‘Sorbonne’ was MS+sucrose 30 g/L+0.8% agar+6-BA 0.8 mg/L+NAA 0.1 g/L，the suitable proliferation medium was MS+sucrose 30 g/L+0.8% agar+6-BA 0.8 mg/L.The optimal formula for the inducing medium of adventitious buds of‘Corvara’was MS+sucrose 30 g/L+0.8% agar+6-BA 0.2 mg/L+NAA 0.1 g/L，the suitable proliferation medium was MS+sucrose 30 g/L+0.8% agar+6-BA 0.6 mg/L.High concentration sucrose was adopted as replacement of hormone for proliferation in the proliferation test of adventitious buds and the optimal formula of medium was MS+sucrose 60 g/L+0.8% agar.The optimal rooting medium was MS+sucrose 30 g/L+0.8% agar+NAA 0.1 g/L.
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备注/Memo
第一作者简介： 吴青青（1982-），男，硕士，助理研究员，研究方向为百合育种与种球繁育。E-mail:2899697856@qq.com.基金项目： 国家自然科学基金资助项目（31160176）；贵州省科技厅资助项目（黔科合J字［2010］2090号,黔科合NY字［2011］3074号）；贵州省农学院专项资助项目（黔农科院院专项［2008］032号）。