TONG Jun,ZHOU Yuan,MAO Jing,et al.Genetic Diversity Analysis of Iris L.Germplasm by ISSR[J].Northern Horticulture,2015,39(10):104-107.[doi:10.11937/bfyy.201510025]
鸢尾属部分园艺品种遗传多样性的ISSR分析
- Title:
- Genetic Diversity Analysis of Iris L.Germplasm by ISSR
- 文章编号:
- 1001-0009(2015)10-0104-04
- Keywords:
- Iris cultivars; ISSR; genetic diversity; relationship
- 分类号:
- S 682.1+9
- 文献标志码:
- A
- 摘要:
- 以34份鸢尾属园艺品种为试材,采用ISSR分子标记方法,研究鸢尾属园艺品种的遗传特性及亲缘关系。结果表明:10个ISSR随机引物检测到153个多态位点,多态性条带比例为100%,表明供试材料具有丰富的遗传多态性。各品种之间的DICE遗传相似系数在0.148~0.657之间,平均相似系数为0.365。由聚类分析可知,当遗传系数为0.354时,可将供试材料分为4个品种群。其中Ⅰ群有21个品种,全部为须毛状附属物亚属的德国鸢尾。Ⅱ群有4个品种,包括无附属物亚属的西伯利亚鸢尾和2个花菖蒲品种。Ⅲ群有4个品种,包括2个花菖蒲品种以及2个从荷兰引进的德国鸢尾。Ⅳ群为无附属物亚属的路易斯安那鸢尾品种群。主坐标分析所反映的关系和UPGMA聚类图相似。
- Abstract:
- Inter-simple sequence repeat (ISSR) was used to detect the genetic diversity among 34 horticultural cultivars of Iris.The results showed that 10 primers produce 153 clear,polymorphic and steady bands.All of bands were polymorphic(100%),which indicated that the genetic diversity of Iris L.was rich.The genetic similarity coefficient among the accessions ranged from 0.148 to 0.657,and the average was 0.365.At the point of the similarity coefficient 0.354,all the accessions might be separated into four groups.The group I consisted by the cultivars of I.germanica.The group II had tow cultivars of I.sibirica and tow of I.ensata.In group III,tow I.ensata and tow of germanica from Holland were included.The IV group was made up with cultivars of I.Louisiana.The results of principal coordinates showed similarity to the UPGMA dendrogram.
参考文献/References:
相似文献/References:
[1]肖志娟,翟梅枝,许静,等.不同核桃品种的ISSR标记分析[J].北方园艺,2013,37(04):103.
[2]刘志曦,李志强,魏海莲,等.平菇ISSR遗传多样性分析[J].北方园艺,2014,38(12):94.
LIU Zhi-xi,LI Zhi-qiang,WEI Hai-lian,et al. Phylogenetic Analysis of Oyster Mushrooms by ISSR Marker[J].Northern Horticulture,2014,38(10):94.
[3]李 娜,张存旭,崔晓燕,等.栓皮栎ISSR反应体系的建立[J].北方园艺,2014,38(13):93.
LI Na,ZHANG Cun-xu,CUI Xiao-yan,et al.Establishment of ISSR Reaction System in Quercus variabilis [J].Northern Horticulture,2014,38(10):93.
[4]王鸿磊,丁 强,吕 蔚,等.我国双孢菇菌株与国外引进菌株间亲缘关系的ISSR分析[J].北方园艺,2014,38(11):96.
WANG Hong-lei,DING qiang,LV Wei,et al.Genetic Relationship Based on ISSR Markers Among Agaricus bisporus Strains From China and Overseas[J].Northern Horticulture,2014,38(10):96.
[5]李 娜,张存旭,刘 伟,等.毛梾ISSR-PCR反应体系的建立[J].北方园艺,2013,37(22):114.
LI Na,ZHANG Cun-xu,LIU Wei,et al.Establishment of ISSRPCR Reaction System of Cornus walteri[J].Northern Horticulture,2013,37(10):114.
[6]黄萌,陈尚钘,杨光耀,等.古樟ISSR扩增条件的优化[J].北方园艺,2013,37(07):116.
HUANG Meng,CHEN Shang-xing,YANG Guang-yao,et al.Optimization of ISSR Amplification Conditions in Ancient Camphor-trees[J].Northern Horticulture,2013,37(10):116.
[7]吴春燕,高义,宋廷宇,等.正交设计优化大白菜ISSR-PCR 反应体系[J].北方园艺,2012,36(05):127.
WU Chun-yan,GAO Yi,SONG Ting-yu,et al.Optimization of an ISSR-PCR Reaction System of Chinese Cabbage by Orthogonal Design[J].Northern Horticulture,2012,36(10):127.
[8]陈名红,李 玉,刘 多,等.适于ISSR-PCR扩增的百合基因组DNA的提取[J].北方园艺,2012,36(24):108.
CHEN Ming-hong,LI Yu,LIU Duo,et al.Extraction of Genomic DNA for ISSR-PCR Reaction in Lilium[J].Northern Horticulture,2012,36(10):108.
[9]张运峰,胡芬.红掌组培再生植株中表型突变体的ISSR分析[J].北方园艺,2014,38(21):101.
ZHANG Yun-feng,HU Fen.ISSR Analysis of Phenotypic Variants in Tissue Culturing Regeneration Plants of Anthurium andraeanum[J].Northern Horticulture,2014,38(10):101.
[10]孙叶迎,陈丽飞,刘树英,等.长白山区杓兰属植物ISSR-PCR最佳反应体系的建立[J].北方园艺,2013,37(24):92.
SUN Ye-ying,CHEN Li-fei,LIU Shu-ying,et al.Establishment of the Optimum ISSR-PCR Reaction System of Cypripedium in the Region of Changbai Mountain of China[J].Northern Horticulture,2013,37(10):92.
备注/Memo
第一作者简介:童俊(1980-),女,湖北黄冈人,硕士,工程师,现主要从事园林植物遗传育种与栽培技术等研究工作。E-mail:vivian7484@126.com.