GAO Xing-wang,WANG Xian-lei,NING Xue-fei,et al.Fine Mapping of Palmately Lobed Leaf Gene pll in Melon[J].Northern Horticulture,2015,39(06):98-102.[doi:10.11937/bfyy.201506027]
甜瓜掌状裂叶基因pll的精细定位
- Title:
- Fine Mapping of Palmately Lobed Leaf Gene pll in Melon
- Keywords:
- palmately lobed leaf; SSR; fine mapping; leaf development
- 文献标志码:
- A
- 摘要:
- 以甜瓜掌状裂叶材料bm7和甜瓜圆叶材料Y8为亲本，构建BC1分离群体作为作图群体，同时利用甜瓜基因组序列，采用SSR分子标记技术，结合混合分离分析法(BSA)，将甜瓜裂叶基因pll进一步定位到SSR标记G61和G76之间65 kp的区间之内。结果表明：在此区域内，共有6个候选基因，从MELO3C010780到MELO3C010785，其中，MELO3C010784与ANT转录因子相似。根据文献报道，在拟南芥中过表达从椰子中克隆的ANT基因导致叶片出现锯齿形边缘，该表型与甜瓜株系bm7的裂叶形状具有相似性。这些结果表明MELO3C010784为最可能的候选基因。
- Abstract:
- A natural mutant named bm7 in melon (Cucumis melo L.)that consists of palmately lobed leaves and a melon line named Y8 that consists of round leaves were used as parental lines to construct BC1 segregating population for fine mapping of the palmately lobed leaf gene pll.Methods of bulk segregant analysis(BSA)and simple sequence repeat (SSR)markers were used to map pll.The pll gene was delimited between SSR markers G61 and G76，and the physical distance was 65 kp according to the melon genome database.The results showed that six genes，MELO3C010780-MELO3C010785，were predicted within this region.Gene MELO3C010784 was similar to the ANT transcription factor.Overexpression of CnANT in Arabidopsis resulted in leaves with serrated margins.This finding indicated that gene MELO3C010784 was maybe the most likely candidate gene for pll.
参考文献/References:
[1]Hirokazu T.Leaf development and evolution[J].Journal of Plant Research，2010，123：3-6. [2]Gonzalez N，Vanhaeren H，Inze D.Leaf size control：complex coordination of cell division and expansion[J].Trends in Plant Science，2012，17(6)：332-340. [3]Li L C，Kang D M，Chen Z L，et al.Hormonal regulation of leaf morphogenesis in Arabidopsis[J].Journal of Integrative Plant Biology，2007，49(1)：75-80. [4]Smith L G，Greene B，Veit B，et al.A dominant mutation in the maize homeobox gene，Knotted-1，causes its ectopic expression in leaf cells with altered fates[J].Development (Cambridge，England)，1992，116(1)：21-30. [5]Nikovics K，Blein T，Peaucelle A，et al.The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis[J].Plant Cell，2006，18(11)：2929-2945. [6]Chuck G，Lincoln C，Hake S.KNAT1 induces lobed leaves with ectopic meristems when overexpressed in Arabidopsis[J].Plant Cell，1996，8：1277-1289. [7]Dean G，Casson S，Lindsey K.KNAT6 gene of Arabidopsis is expressed in roots and is required for correct lateral root formation[J].Plant Molecular Biology,2004，54(1)：71-84. [8]Hay A，Jackson D，Ori N，et al.Analysis of the competence to respond to KNOTTED1 activity in Arabidopsis leaves using a steroid induction system[J].Plant Physiol，2003，131(4)：1671-1680. [9]Lincoln C，Long J，Yamaguchi J，et al.A knotted1-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants[J].Plant Cell，1994，6：1859-1876. [10]Pautot V，Dockx J，Hamant O，et al.KNAT2 Evidence for a Link between Knotted-like Genes and Carpel Development[J].Plant Cell，2001，13(8)：1719-1734. [11]Niklas K J.The effect of leaf-lobing on the interception of direct solar radiation[J].Oecologia，1989，80(1)：59-64. [12]Ali M S，Kikuzawa K.Shoot morphology of Aucuba japonica incurred by anisophylly：ecological implications[J].Journal of Plant Research，2005，118(5)：329-338. [13]Vogel S.Leaves in the lowest and highest winds：temperature，force and shape[J].New Phytologist,2009,183(1)：13-26. [14]Barber J.Photosynthetic energy conversion：natural and artificial[J].Chemical Society Reviews，2009，38(1)：185-196. [15]Zhu X G，Long S P，Ort D R.Improving photosynthetic efficiency for greater yield[J].Annual Review of Plant Biology，2010，61：235-261. [16]Wells R，Meredith W R，Williford J R.Canopy photosynthesis and its relationship to plant productivity in near-isogenic cotton lines differing in leaf morphology[J].Plant Physiology，1986，82(3)：635-640. [17]Gao X X，Ning X F.Fine mapping of a gene that confers palmately lobed leaf (pll)in melon (Cucumis melo L.)[J].Euphytica，2014，200:337-347. [18]Li Q,Wan J M.SSR Hunter：Development of a local searching software for SSR sites[J].Yichuan，2005，27(5)：808-810. [19]Lalitha S.Primer premier 5.Biotech Software & Internet Report：The Computer Software [J].Journal for Scient，2000，1(6)：270-272. [20]Michelmore R W，Paran I，Kesseli R V.Identification of markers linked to disease-resistance genes by bulked segregant analysis：A rapid method to detect markers in specific genomic regions by using segregating populations[J].Proc Natl Acad Sci USA，1991，88：9828-9832. [21]Bandupriya H，Dunwell J M.Overexpression of CnANT，coconut babyboom homologue alters plant growth and morphology in transgenic Arabidopsis plants[J].Tropical Agricultural Research，2012，23(3)：249-260.
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备注/Memo
第一作者简介：高兴旺（1983-），男，博士，现主要从事植物分子生物学等研究工作。E-mail：gxw516@163.com. 责任作者：李冠（1949-），男，硕士，教授，博士生导师，现主要从事植物生理生化与分子生物学等研究工作。E-mail:guanli@xju.edu.cn. 基金项目：国家自然科学基金资助项目(31260258)；新疆高技术发展资助项目(201111120)。收稿日期：2014-11-10