CHEN Yu-qin,NAN Hai-juan,LIU Kun-feng.Extraction Technology and Antioxidant Acitvity of Polysaccharide From Pteridium revolutum [J].Northern Horticulture,2014,38(14):128-132.
蕨菜多糖提取及抗氧化特性
- Title:
- Extraction Technology and Antioxidant Acitvity of Polysaccharide From Pteridium revolutum
- 文章编号:
- 1001-0009(2014)14-0128-05
- 分类号:
- TS 247
- 文献标志码:
- A
- 摘要:
- 以粉碎后的蕨菜为原料，以水为溶剂，恒温回流提取多糖，在单因素试验的基础上，采用响应面分析来优化蕨菜多糖提取的生产工艺。从总还原力、DPPH自由基清除能力、羟基自由基清除能力、超氧阴离子清除能力及抗亚油酸氧化能力5个方面研究了蕨菜多糖的抗氧化特性，同时与维生素C，2,6-二叔丁基-4-甲基苯酚（BHT）相比较。结果表明：多糖最佳提取条件为温度93℃、料液比1∶34 g/mL、时间115 min;蕨菜多糖的抗氧化活性随浓度的升高而增大，但低于维生素C和BHT的抗氧化能力。
- Abstract:
- Taking Pteridium revolutum as material,using water as solvent,based on single factor analysis method,the optimal technological requirements of extracting polysaccharide from?Pteridium revolutum??were investigated by using response surface analysis.The antioxidant activities of polysaccharide were analyzed from the total reducing power,scavenging ability on DPPH free radical,scavenging ability on hydroxyl radicals,scavenging ability on superoxide radical,linoleic acid oxidation ability.The results showed that the optimum process of the extraction technology was extraction time 115 min,extraction temperature 93℃ and the ratio of material to liquid 1∶34 g/mL.The results also showed that antioxidant activities of polysaccharide increased with the enhancement of concentration,but was lower than vitamin C and BHT.
参考文献/References:
[1]王斯琪．蕨菜的开发与利用[J]，黑龙江生态工程职业学报，2007，20(6)：23-24．
[2] Jiang CC，郝丽珍，邵世勤，等，蕨菜的营养成分及其利用研究[C].中国园艺学会第四届青年学术讨论会论文集，2000：17-24．
[3]毛建兰，刘波．蕨菜的营养价值及加工技术[J].农技服务，2008，25(2)：103-104．
[4]李青萍，常明昌，张浩，等．山西野生蕨菜营养价值的初步研究[J].山西农业大学学报，1999，19(1)：31-32．
[5]钟岩，潘浦群，王艳红，等，苯酚一硫酸法测定鲜人参中多糖含量[J]．时珍国医国药，2008，19(8)：1957-1958．
[6]王影，陈晓平．绿豆芽总黄酮的提取工艺研究[J].食品与发酵科技，46(5)：45-48．
[7]李波，索晓敏，芦菲，等，鸡腿菇菌柄多糖提取工艺的研究[J].食品工业科技，2010(5)：238-239．
[8] Xu W T, ZhangF F,Huang K L Application of response surface meth-odology for extraction optimization of water- soluble polysaccharides from Pteridium aquilinum [J]. Bromatology ,2008. 29(7):122-126.
[9]壬明艳，鲁加峰，王晓顺，等，响应面法优化天冬多糖的提取条件[J].食品科学，2007，28(10)：200-203．
[IO]许文涛，张方方．黄昆仑，等，响应曲面法优化蕨菜水溶性多糖提取工艺的研究[J].食品科学，2008 (8)：326-348.
[II]邱松山，周天，姜翠翠，无花果粗多糖提取工艺及抗氧化活性研究[J].食品与机械，2007，27(1)：40-42.
[11]于曙光，蒋海燕，任春艳，等．蕨菜粗多糖的抗氧化活性研究[J]．莱阳农学院学报（自然科学版），2006，23 (1)：41-43．
[13]陈美珍，王欣，余杰，龙须菜多糖的提取、分离及抗氧化活性的研究[J].汕头大学学报（自然科学版），2005，20(2)：37-42.
[14]李军，蒋碧蓉，吴红梅，等．海星多糖的提取分离与抗氧化活性研究[J]．广东化工，2011，37(2)：188-193．
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备注/Memo
第一作者简介：陈玉琴（1974-），女，河南三门峡人，硕士，讲师，研究方向为园林植物应用。E-mail：yqc0714@126.com