[1]武冬梅,许文涛,谢宗铭,等.新疆伊犁昭苏野生羊肚菌分离株的分子鉴定研究[J].北方园艺,2014,38(21):145-148.
　WU Dong-mei,XU Wen-tao,XIE Zong-ming,et al.Molecular Identification of Morchella in Xinjiang Yili[J].Northern Horticulture,2014,38(21):145-148.
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新疆伊犁昭苏野生羊肚菌分离株的分子鉴定研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年21期 页码: 145-148 栏目: 出版日期: 2014-11-15
Title:
Molecular Identification of Morchella in Xinjiang Yili
文章编号:
1001-0009（2014）21-0145-04
作者:
武冬梅1; 2; 许文涛2; 3; 谢宗铭1; 李全胜1; 罗云波2; 3
1.新疆农垦科学院 分子农业技术育种中心，作物种质创新与基因资源利用兵团重点实验室，新疆 石河子 832000；
2.中国农业大学 食品科学与营养工程学院，北京 100083；
3.农业部转基因生物食用安全监督检验测试中心（北京），北京 100083
Author(s):
WU Dong-mei 1; 2; XU Wen-tao 2; 3; XIE Zong-ming1; LI Quan-sheng1; LUO Yun-bo 2; 3
1.Center for Molecular Agrobiotechnology and Breeding,Xinjiang Academy of Agricultural and Reclamation Sciences,Xinjiang Production and Construction Group Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization,Shihezi,Xinjiang 832000；
2.College of Food Science and Nutrition Engineering,China Agricultural University,Beijing 100083;
3.The Supervision,Inspection and Testing Center of Genetically Modified Food Safety,Ministry of Agriculture,Beijing 100083
关键词:
羊肚菌; 分子鉴定; 内转录间隔区（ITS）
Keywords:
Morchella; molecular identification; internal transcribed spacer sequence
分类号:
S 646.7
文献标志码:
A
摘要:
以羊肚菌为试材，采用组织分离法分别从3株野生羊肚菌子实体分离得到性状稳定的菌丝体，根据子实体形态特征并结合ITS序列分析对分离株进行鉴定。结果表明：3株分离株均与黑脉羊肚菌同源性最高达99.0%；系统发育分析显示，分离获得的3株野生羊肚菌分离株亲缘关系较近，均聚在黑脉羊肚菌分支上。
Abstract:
Taking Morchella as material,three Morchella strains were isolated from wild Morchella by using standard tissue culture method,and identified by morphological characteristics and internal transcribed spacer sequence analysis,respectively.The results showed that these strains manifested high sequence similarity with Morchella angusticeps,with similarity of 99.0%;the phylogenetic tree also suggested that these strains belonged to Morchella angusticeps.

参考文献/References:

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备注/Memo

第一作者简介：武冬梅（1976-），女，硕士，副研究员，现主要从事应用微生物等研究工作。E-mail:wdm0999123@sina.com.
责任作者：罗云波（1960-），男，博士，教授，现主要从事食品安全与食品生物技术及果蔬贮藏保鲜等研究工作。E-mail：lyb@cau.edu.cn.
基金项目：国家自然科学基金资助项目（31160011）；国家星火计划资助项目（2013GA891001）。
收稿日期：2014-07-14

更新日期/Last Update: 2014-12-01