[1]胡月芳.淮山多糖微波法提取及清除羟基自由基的研究[J].北方园艺,2014,38(02):135-137.
　HU Yue-fang.Study on the Microwave Extraction and Hydroxy Radical Scavenging of Yam Polysaccharide[J].Northern Horticulture,2014,38(02):135-137.
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淮山多糖微波法提取及清除羟基自由基的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年02期 页码: 135-137 栏目: 出版日期: 2014-01-31
Title:
Study on the Microwave Extraction and Hydroxy Radical Scavenging of Yam Polysaccharide
文章编号:
1001-0009(2014)02-0135-03
作者:
胡月芳
贺州学院 化学与生物工程学院，广西 贺州 542899
Author(s):
HU Yue-fang
yam；polysaccharide；microwave extraction；hydroxyl radicals
关键词:
淮山; 多糖; 微波提取; 羟基自由基
Keywords:
yam; polysaccharide; microwave extraction; hydroxyl radicals
分类号:
S 632. 1
文献标志码:
A
摘要:
以淮山为试材，采用微波法提取其多糖，设计L9(34)正交实验考察提取时间、提取功率、料液比、提取次数对淮山多糖提取率的影响，并通过分光光度法考察其清除羟基自由基的能力。结果表明：淮山多糖的最佳提取工艺为提取时间为6 min、提取功率为400 W、料液比为1∶20 g/mL、提取次数为3次。在此条件下，多糖的提取率最高，达6. 32%，试验结果还表明淮山多糖具有较好的清除羟基自由基的能力，且其清除能力与多糖浓度有明显的量效关系。
Abstract:
Taking yam as material,polysaccharide was extracted form yam by microwave,and L9(34) orthogonal experiment based on extraction time，extraction power，material-liquid ratio and extraction times was tested to optimize the extraction technology.The hydroxyl radical scavenging effect was studied by spectrophotometry method.The results showed that the best extracting technology was extraction time for 6 min，extraction power for 400 W，material-liquid ratio of 1∶20 g/mL and extraction times was 3 times.Under these conditions，the yield of yam polysaccharide was 6. 32%.The results showed that the yam polysaccharide had the good ability to scavenge the hydroxyl radicals，which had the dose-effect relationship between the concentration of yam polysaccharide.

参考文献/References:

[1]雷伏贵,华树妹,涂前程,等.山药种质资源亲缘关系的ISSR分析[J].福建农业学报,2013,28(1)：27-32．

[2]贾艳.山药多糖的药理学研究[J].中国医药指南,2012,10(9)：54-55．

[3]张卫明,单承莺,杭悦宇,等.温度因素对薯蓣多糖测定的影响研究[J].食品科学,2008,29(5)：393-395．

[4]胡国强,杨保华,张忠泉.山药多糖对大鼠血糖及胰岛释放的影响[J].山东中药杂志,2004,23(4)：230-23.

[5]盛玮,薛建平,谢笔钧.怀山药零余子提取物抗氧化活性的研究[J].食品科学,2009,30(3)：92-94．

[6]张留记,屠万倩,都恒青.薄层扫描法测定怀山药降糖肠胃舒散中尿囊素的含量[J].中国中药杂志,2001,26(8)：567-570．

[7]李明静,宋爱新,史会齐,等.高效液相色谱法测定山药样品中尿囊素的含量[J].化学研究,2003,14(1)：67-70．

[8]王海波,蔡宝昌.反相高效液相色谱法测定不同产地山药中尿囊素的含量[J].中药新药与临床药理,2004,15(3)：190-193．

[9]胡月芳,李建平,黄志强.毛细管电泳-电致化学发光法测定淮山药中胆碱和尿囊素[J].分析试验室,2012,31(12):104-107．〖HT〗〖HJ〗


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备注/Memo

作者简介：胡月芳(1977-)，女，硕士，讲师，研究方向为食品科学与电化学分析。E-mail：huyuefang@126.com.

基金项目：广西高校科学技术研究资助项目(2013YB238)。

更新日期/Last Update: 2014-08-10