QIN Li,JIAN Wen-lei,CHOE Il-su,et al.Study on Resistance of Asparagus officinalis L.Against Stem Blight Induced by Chitosan[J].Northern Horticulture,2014,38(20):121-125.
壳聚糖诱导芦笋对茎枯病的抗性研究
- Title:
- Study on Resistance of Asparagus officinalis L.Against Stem Blight Induced by Chitosan
- 文章编号:
- 1001-0009（2014）20-0121-05
- Keywords:
- Asparagus officinalis L.; chitosan; stem blight; resistance
- 分类号:
- S 644.6
- 文献标志码:
- A
- 摘要:
- 以芦笋为试材，对接种茎枯病病原菌后的芦笋在不同时期喷施壳聚糖处理，并研究了发病情况、叶片光合作用与抗氧化酶活性的变化。结果表明:喷施壳聚糖后，芦笋茎枯病发病率及发病指数均显著降低，且以接种病原菌后立即喷施壳聚糖效果最好，芦笋体内过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、愈创木酚过氧化物酶（G-POD）、多酚氧化酶（PPO）的活性升高，接种病原菌造成的拟叶光合速率和气孔导度下降现象也得到明显缓解。表明壳聚糖能诱导芦笋茎枯病抗性，降低病原菌对其造成的伤害。
- Abstract:
- Taking Asparagus officinalis L.as material，the different asparagus cultivars were used and their seedlings were inoculated with Phomopsis aspasagi (Sacc）Bubak.The chitosan were sprayed in different periods after inoculation.Morbidity and disease index were surveyed after the treatment.The changes of antioxidant enzyme activities were compared.The photosynthetic rate and stomatal conductance of intended leaves were also determined.The results showed that the disease index significantly reduced with chitosan-supply，and the best effect obtained byspraying chitosan immidietly after inoculation.The activity of catalase（CAT)，guaiacol peroxidase(G-POD)，superoxide dismutase(SOD)，polyphenoloxidase（PPO）activity of Asparagus officinalis L.increased.On the other hand，the decline of photosynthetic rate and stomatal conductance caused by inoculation was suppressed after supplied with the chitosan.It could be concluded that chitosan could induce systemic resistance on asparagus against stem blight，reduce the damage caused by pathogens,and resulted in beneficial effects on asparagus production.
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备注/Memo
第一作者简介：秦力（1989-），男，硕士研究生，研究方向为蔬菜栽培。E-mail：qinli19890720@163.com.
责任作者：卢钢（1968-），男，博士，教授，现主要从事蔬菜学等研究工作。E-mail：glu@zju.cn.
基金项目：国家“十二五”科技支撑计划资助项目（2011BAD12B04）；国家公益性行业（农业）科研专项资助项目（201003074-3）;浙江省重点资助项目（2012C12012-3）。
收稿日期：2014-06-10