LIU Zhu,CHEN Jun-xia.Effect on Optimization of Ultrasonic-assisted Extraction of Lectin from Volvariella volvacea Mycelium Using Response Surface Methodology[J].Northern Horticulture,2014,38(19):138-141.
响应面法优化草菇菌丝凝集素超声波提取工艺研究
- Title:
- Effect on Optimization of Ultrasonic-assisted Extraction of Lectin from Volvariella volvacea Mycelium Using Response Surface Methodology
- 文章编号:
- 1001-0009（2014）19-0138-04
- Keywords:
- Volvariella volvacea mycelium; lectin; ultrasonic-assisted extraction; response surface analysis
- 分类号:
- S 646
- 文献标志码:
- A
- 摘要:
- 以草菇菌丝体为试材，以凝集素提取液的总活力为指标，在单因素试验的基础上，利用3因素3水平的Box-Benhnken中心组合试验和响应面分析法设计优化了草菇菌丝凝集素的超声波提取工艺。结果表明：超声功率为310 W、超声处理时间为36 min、液料比为4.5∶1，是草菇菌丝凝集素超声波提取的最佳工艺；在该条件下，其20 g草菇菌丝的凝集素提取液的总活力为76 386 U。
- Abstract:
- Taking mycelium of Volvariella volvacea as material,with total lectin activity of extracting solution as the indicator,based on the single factor experiments,the ultrasonic-assisted extraction technology of Volvariella volvacea mycelium lectin was optimization by Box-Benhnken central composite design and response surface methodology.The results showed that the optimal extraction conditions were ultrasonic power 310 W,extraction time 36 min and liquid-solid ratio 4.5∶1.Under these conditions,the total lectin activity of extracting solution of 20 g Volvariella volvacea mycelium was 76 386 U.
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备注/Memo
第一作者简介：刘主（1977-），男，湖南宁远人，博士，副教授，现主要从事生物化学与分子生物学等研究工作。E-mail：liuzhu77@126.com.
基金项目：韶关学院科研资助项目（2011-12）；韶关学院大学生创新创业训练计划资助项目（sycxcy2012-007）。
收稿日期：2014-05-19