番茄hp1和dg基因高效检测体系的建立
- 摘要:
- 根据NCBI中提供的番茄红素单碱基突变基因hp1和dg的序列,设计特异性引物。利用同一PCR反应体系同时扩增筛选番茄红素基因hp1和dg,以及野生型基因HP1和DG。结果表明:扩增的特异性片段与单基因引物扩增片段完全吻合。SNP分子标记为单显性标记,突变基因纯合体只在突变材料PCR体系中产生298和870bp的特异性片段,野生基因纯合体只在野生材料PCR体系中产生298和870bp的特异性片段,杂合体在2个反应体系中均有特异性片段。利用该体系在F2代中筛选出同时含hp1和dg基因的纯合植株,大大节省了工作量,提高了准确度。
相似文献/References:
[1]吴晓燕,张丽荣,马建华,等.一种新型微生物制剂对设施番茄土壤微生物数量的影响[J].北方园艺,2013,37(04):42.
[2]王晓娥,李建国,赵姝荣,等.咸阳市设施番茄根结线虫病的发生与防治[J].北方园艺,2013,37(04):133.
[3]谷婧玥,李景富,许向阳,等.番茄不同果穗间品质性状差异性研究[J].北方园艺,2014,38(12):26.
GU Jing-yue,LI Jing-fu,XU Xiang-yang,et al.Study on Tomato’s Quality and Traits at Different Tassel[J].Northern Horticulture,2014,38(04):26.
[4]吕 涛,于 杰,赵 勇.日光温室越冬栽培番茄适宜品种筛选试验[J].北方园艺,2014,38(12):40.
LV Tao,YU Jie,ZHAO Yong.Selection of Overwintering Tomato Varieties Suitable for Solar Greenhouse in Heilongjiang[J].Northern Horticulture,2014,38(04):40.
[5]李美芹,裴华丽,乔 宁,等.TYLCV外壳蛋白基因植物表达载体构建及转化番茄的研究[J].北方园艺,2014,38(12):84.
LI Mei-qin,PEI Hua-li,QIAO Ning,et al.Study on the Construction of Plant Expression Vector of TYLCV-CP Gene and Its Transferring Into Tomato[J].Northern Horticulture,2014,38(04):84.
[6]张 浩,李福云,徐志然,等.不同套作模式对温室连作番茄生长产量及土壤微生物与酶活性的影响[J].北方园艺,2014,38(13):46.
ZHANG Hao,LI Fu-yun,XU Zhi-ran,et al.Effects of Different Relay Intercropping Patterns on Continuous Cropping Tomato Growth，Yield and Soil Microflora and Enzymes Activities in Solar Greenhouse[J].Northern Horticulture,2014,38(04):46.
[7]杨会芳,梁新安,常介田,等.叶面喷施硒肥对不同蔬菜硒富集及产量的影响[J].北方园艺,2014,38(11):158.
YANG Hui-fang,LIANG Xin-an,CHANG Jie-tian,et al.Effects of Foliar Spraying of Selenium Fertilizer on Selenium Enrichment of Different Vegetables and Yield[J].Northern Horticulture,2014,38(04):158.
[8]祁连弟,管建慧,其日格,等.不同比例牛羊粪便栽培基质对温室番茄苗期生长的影响[J].北方园艺,2013,37(22):47.
QI Lian-di,GUAN Jian-hui,QI Ri-ge,et al.Effects of Different Nursery Substrates with Cow and Sheep Dung on the Seedlings Growth of Tomato in Solar Greenhouse[J].Northern Horticulture,2013,37(04):47.
[9]肖庆红,马 倩,张 燕,等.S-诱抗素和新奥霉素在日光温室番茄栽培上的应用研究[J].北方园艺,2013,37(22):53.
XIAO Qing-hong,MA Qian,ZHANG Yan,et al.Study on S-auxin and Xinaomycin in Tomato Cultivation in Solar Greenhouse[J].Northern Horticulture,2013,37(04):53.
[10]何志刚,王秀娟,董 环,等.秸秆反应堆和功能菌对日光温室番茄生长发育及土壤微生态环境的影响[J].北方园艺,2013,37(22):184.
HE Zhi-gang,WANG Xiu-juan,DONG Huan,et al.Effects of Straw Reactors and Function Microorganism on Growth and Soil Micro-eco-environment of Tomato[J].Northern Horticulture,2013,37(04):184.
[11]李文枫,李景富,许向阳.不同hp基因类型番茄中番茄红素与主要品质性状的差异及相关分析[J].北方园艺,2014,38(13):97.
LI Wen-feng,LI Jing-fu,XU Xiang-yang.Correlation and Difference Analysis Between Lycopene Content and Main Quality Characters of Tomato with Different hp Gene Types[J].Northern Horticulture,2014,38(04):97.
[12]唐政,陈小香,伍淑婕.水肥管理对有机番茄产量与番茄红素含量的影响[J].北方园艺,2014,38(05):166.
TANG Zheng,CHEN Xiao-xiang,WU Shu-jie.Effect of Different Water and Organic Fertilizer Management on the Yield of Organic Tomato and Contents of Lycopene in Organic Tomato[J].Northern Horticulture,2014,38(04):166.
备注/Memo
基金:国家科技部“863”计划资助项目(2007AA10Z-178)