[1]麻冬梅,王静,金凤霞,等.苜蓿子叶节离体再生体系的建立[J].北方园艺,2013,37(14):104-106.
　MA Dong-mei,WANG Jing,JIN Feng-xia,et al.Establishment of Regeneration System in vitro from Cotyledonary Nodes of Medicago sativa L.[J].Northern Horticulture,2013,37(14):104-106.
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苜蓿子叶节离体再生体系的建立

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年14期 页码: 104-106 栏目: 出版日期: 2013-07-15
Title:
Establishment of Regeneration System in vitro from Cotyledonary Nodes of Medicago sativa L.
文章编号:
1001-0009(2013)14-0104-03
作者:
麻冬梅1; 王静2; 金凤霞1; 郭林娜2; 许兴1; 2
1.宁夏大学 农学院，宁夏 银川 750021；
2.宁夏大学 生命科学学院，宁夏 银川 750021
Author(s):
MA Dong-mei1; WANG Jing2; JIN Feng-xia1; GUO Lin-na2; XU Xing1; 2
1.College of Agriculture,Ningxia University,Yinchuan,Ningxia 750021；
2.College of Life Science,Ningxia University,Yinchuan,Ningxia 750021
关键词:
苜蓿; 子叶节; 丛生芽; 再生体系
Keywords:
Medicago sativa L.; cotyledonary node; adventitious shoot; regeneration system
分类号:
S 541+.1
文献标志码:
A
摘要:
以苜蓿子叶节为外植体，以MS为基本培养基，研究了不同浓度激素对苜蓿离体再生体系中子叶节外植体分化、不定芽诱导、不定芽伸长及生根的影响，以期建立高效的苜蓿子叶节离体再生体系。结果表明：苜蓿子叶节再生最佳萌发培养基为MS；最佳诱导培养基为MS+1.0 mg/L 6BA，诱导时间为7～10d；不定芽伸长的最佳培养基为MS+0.5 mg/L 6BA；最佳生根培养基为1/2MS+0.1 mg/L IBA，15 d即可生根。
Abstract:
Taking the cotyledonary nodes of Medicago sativa as explants and MS as the basic medium,the effect of different proportion of hormones on explants differentiation,adventitious bud induction,elongation and rooting were studied.The results showed that the optimum medium for alfalfa cotyledon node regeneration：germination medium was MS；the best induction medium was MS+1.0 mg/L 6BA,with induction time 7 to 10 d；the best adventitious shoot elongation medium was MS+0.5 mg/L 6BA；the best rooting medium was 1/2MS+0.1 mg/L IBA,root could be formed in 15 days.

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备注/Memo

第一作者简介：麻冬梅（1978-），女，宁夏银川人，博士，讲师，现主要从事植物抗逆分子生物学研究工作。E-mail:mdm319@tom.com.

责任作者：许兴（1959-），男，博士，教授，博士生导师，现主要从事作物逆境生理及生物技术育种等研究工作。
基金项目：国家“十一五”科技支撑计划资助项目（2011BAC07B03）；国家“973”资助项目（2012CB114204）；宁夏农发办资助项目（NTKJ-2012-12）。
收稿日期：2013-03-07

更新日期/Last Update: 2014-08-10