LI Hui-wen,MA Dong-mei,XU Xing.Advance and Prospects on Salt-tolerant Transgenge Alfalfa[J].Northern Horticulture,2012,36(19):184-188.
苜蓿耐盐碱转基因研究进展与展望
- Title:
- Advance and Prospects on Salt-tolerant Transgenge Alfalfa
- 文章编号:
- 1001-0009(2012)19-0184-05
- Keywords:
- alfalfa; salt-tolerant; transgene; advance
- 分类号:
- S 551.+7
- 文献标志码:
- A
- 摘要:
- 土壤的盐碱化极大的影响苜蓿的生长，该文综述了通过基因工程来提高苜蓿耐盐碱能力方面的研究进展和通过基因工程的方法获得的一些耐盐苜蓿新材料；分析了当前耐盐碱转基因苜蓿研究中存在的一些问题；展望了未来研究的方向。
- Abstract:
- Salinization of the soil greatly affects the growth of alfalfa.In this paper，the research progress through genetic engineering to improve the ability of alfalfa salinity and some new salt-tolerant alfalfa material obtained through genetic engineering methods were reviewed,and some problems in currently study of salinity tolerance of transgenic alfalfa were analyzed,the future research trend was prospected.
参考文献/References:
[1]Sengupta-Gopalan C，Bagga S，Potenza C，et al.Alfalfa[J].Biotechnology in Agriculture and Forestry,2007，61:321-335. [2]梁云媚,多立安,李燕,等.不同盐分胁迫对苜蓿种子萌发的影响[J].草业科学,1998,15(6):21-25． [3]Saunders J W,Bingham E T.Production of alfalfa plants from callus tissue[J].Crop Sei,1972,12:804-808. [4]Deak M,Kissv.Agrobacterium mediated gene transfer[J].Plant Cell ReP,1986(5):97-100. [5]Winieov I,Bastola C R.Transgenic overexpression of the transeription Faetor Alfinl enhances expression of the endogenous MsPRP2 gene in alfalfa and improves salinity tolerance of Plants[J].Plant Phys,1990,120:473-480. [6]陈晨,乔代蓉,白林含,等.农杆菌介导的杜氏盐藻Dscbr基因转化紫花苜蓿的初步研究[J].四川大学学报(自然科学版),2005,42(3):567-570. [7]梁慧敏,夏阳,孙仲序,等.根癌农杆菌介导苜蓿遗传转化体系的建立[J].农业生物技术学报,2005,13(2):152-156. [8]燕丽萍,夏阳,毛秀红,等.转BADH基因紫花苜蓿山苜2号品种的抗盐性鉴定及系统选育[J].植物学报,2011(3):63-71. [9]马晖玲.紫花苜蓿抗旱耐盐碱转基因植株选育的研究[D].兰州:甘肃农业大学,2004. [10]晏石娟,马晖玲,曹致中.紫花苜蓿抗早耐盐碱转基因抗性苗耐盐性研究[J].甘肃农业大学学报,2006,10(5):91-94. [11]王强龙,王锁民,张金林,等.根癌农杆菌介导AtNHX1基因转化紫花苜蓿的研究[J].草业科学,2006,23(12):55-59. [12]邓程君.农杆菌介导的NTHK1基因转化苜蓿增大叶片及提高耐盐性的研究[D].北京:中国农业大学,2006. [13]隋昕.碱蓬液泡型Na+/H+逆向运输蛋白基因的克隆及其向苜蓿中的转化[D].长春:吉林农业大学,2007. [14]潘竟丽,张富春.农杆菌介导灰绿黎Cg-NXH1基因转化新疆大叶苜蓿的研究[J].新疆农业科学,2007,44(4):418-422. [15]赵红娟,张博,李培英.农杆菌介导犁苞滨藜NHX基因转化苜蓿的影响因素[J].草地学报,2007,15(5):418-422. [16]盛慧,朱延明,李杰,等.DREB2A基因对苜蓿遗传转化的研究[J].草业科学,2007,24(3):40-45. [17]李世林,杨舸,易秀莉,等.根癌农杆菌介导的盐生杜氏藻DsNRT2基因转化紫花苜蓿的初步研究[J].四川大学学报(自然科学版),2008,45(2):409-412. [18]刘小琳,康俊梅,孙彦,等.MsNHX1基因转化紫花苜蓿及转基因植株的鉴定[J].草地学报,2008(2):115-120. [19]王玉祥,王涛,张博.转rstB基因苜蓿耐盐性初评(简报)[J].草地学报,2008,16(5):539-541. [20]Bao A K，Wang S M，Wu G Q,et al.Overexpression of the Arabidopsis H+-PPase?enhanced resistance to salt and drought stress intransgenic alfalfa(Medicago sativa?L.) [J].Plant Science,2009,176:232-238. [21]Ramón Suárez，Cecilia Calderón，Gabriel Iturriaga.Enhanced Tolerance to Multiple Abiotic Stresses in Transgenic Alfalfa Accumulating Trehalose[J].Crop Science，2009,49:1791-1799. [22]Jin T C，Chang Q，Li W F，et al.Stress-inducible expression of GmDREB1 conferred salt tolerance in transgenic alfalfa[J].Plant Cell Tiss Organ Culture，2010:100:219-227. [23]Liu Z H，Zhang H M，Li G L，et al.Enhancement of salt tolerance in alfalfa transformed with the gene encoding for betaine aldehyde dehydrogenase[J].Euphytica,2011,178:363-372. [24]Li W F，Wang D，Jin T C,et al.The Vacuolar Na+/H+ Antiporter Gene SsNHX1 from the Halophyte Salsola soda Confers Salt Tolerance in Transgenic Alfalfa (Medicago sativa L.) [J].Plant Mol Biol Rep,2011,29:278-290. [25]Hua Y，Zhang B X，Cai H，et al.Stress-inducible expression of GsSAMS2 enhances salt tolerance in transgenic Medicago sativa[J].African Journal of Biotechnology，2012,11(17):4030-4038. [26]Pereira L F,Eriekson L.Stable transformation of alfalfa by Partiele bombardment[J].Plant Cell RePorts,1995(2):290-295. [27]Ramaiah S M,Skirmer D Z,Shashi M Ramaiah,et al.Particle bombardment：a simple and efficient method of alfalfa(Medicago sativa L.) Pollen transformation[J].Current Science,1997,73(8):674-682. [28]王瑛,朱宝成,孙毅.外源lea3基因转化紫花苜蓿的研究[J].核农学报,2007,21(3):249-252. [29]肖荷霞.苜蓿转化再生体系的建立及LEA3基因研究[D].石家庄:河北大学,2005. [30]牛一丁,霍朝霞,哈斯阿古拉,等.紫花苜蓿花粉管通道法转基因技术初探[J].中国草地学报,2009,31(1):36-39. [31]张立全,牛一丁,郝金凤,等.通过花粉管通道法导入红树总DNA获得耐盐紫花苜蓿T0代植株及其RAPD验证[J].草业学报,2011(3)：292-297. [32]刘明,杨君,安利佳.花粉管通道法转化影响因素的分析[J].安徽农业科学,2002,35(12):3483-3523. [33]许仕珍.紫花苜蓿不同再生体系的比较及农杆菌介导的AtNHX1基因转化研究[D].兰州:甘肃农业大学,2006. [34]Reich T J,Lyer V N,Mikib L.Efficient Transformation of Alfalfa Protoplasts by the Intranuclear Microinjection of Ti-plasmids[J].Bio Technology,1986(4):1001-1004. [35]Kuchuke N.Plant Genic Transfer [J].Plant Cell Rep,1990(8):660-663. [36]张建军,金湘,徐建辉,等.氩离子注入介导甘草总DNA在阿尔冈金紫花苜蓿中转化的初步研究[J].种子,2008,27(4):57-59. [37]杨起简,周禾,孙彦,等.紫花苜蓿的愈伤组织诱导及组织培养[J].北京农学院学报,2004,19(2):29-31. [38]Samac D A.Strain specificity in transformation of alfalfa by Agrobacterium tumefaciens[J].?Plant Cell Tiss Org Cult,1995,43:271-277. [39]黎茵,黄霞,黄学林.根癌农杆菌介导的苜蓿体胚转化[J].植物生理与分子生物学学报,2003,29(2):109-113. [40]刘文婷,段琦梅,刘景玲,等.农杆菌介导的苜蓿次级体细胞胚的遗传转化[J].生物工程学报，2012,28(2):203-213. [41]王超.农杆菌介导的碱蓬液泡膜H+-ATPase基因转化苜蓿的研究[D].长春:吉林农业大学,2011. [42]Anthony T T,Stephen H B,Kardailsky V,et al.Transformation of Medicago truncatula via infiltration of seeding or flowering plants with Agrobacterium [J].Plant J,2000,22:531-541. [43]马忠强,尹航.转基因技术在作物育种中的应用[J].现代化农业,2009(10):25-26. [44]Ferradini N，Nicolia A，Capomaccio S,et al.Assessment of simple marker-free genetic transformation techniques in alfalfa[J].Plant Cell Rep,2011,30(11):1991-2000. [45]王相春,程在全,曾千春,等.植物筛选标记基因应用进展[J].安徽农业科学,2011,39(22):13290-13291,13365. [46]Malik K,Wu K,Li X Q,et al.A constitutive gene expression system derived from the tCUP cryptic promoter elements[J].Theor Appl Genet,2002,105:505-514. [47]Khoudi H，Vézina L P，Mercier J，et al.An alfalfa rubisco small subunit homologue shares cis-acting elements with the regulatory sequences of the RbcS-3A gene from pea[J].Gene,1997,197:343-351. [48]Kasuga M，Liu Q，Miura S，et al.Improving plant drought，salt，and freezing tolerance by gene transfer of a single stress-inducible transcription factor[J].Nat Biotechnol,1999,17:287-291.
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备注/Memo
第一作者简介：李会文（1985-），男，甘肃武威人，硕士，现主要从事植物基因工程等研究工作。E-mail：lhw19851021@163.com.