CHANG Yun-xia,XU Ke-dong,CHEN Can,et al.Effects of Salicylic Acid on Several Physiological Indexes Related to Cold Resistance of Cucumber Seedling Leaf Under Low Temperature Stress[J].Northern Horticulture,2013,37(12):1-4.
水杨酸对低温胁迫下黄瓜幼苗叶片抗寒生理指标的影响
- Title:
- Effects of Salicylic Acid on Several Physiological Indexes Related to Cold Resistance of Cucumber Seedling Leaf Under Low Temperature Stress
- 文章编号:
- 1001-0009(2013)12-0001-04
- 分类号:
- S 642.2
- 文献标志码:
- A
- 摘要:
- 以“津研2号”黄瓜为试材，采用水培方法，研究了不同浓度的水杨酸（Salicylic acid，SA）对4℃低温胁迫下黄瓜幼苗生长的影响，以期为黄瓜抗低温胁迫研究提供参考。结果表明：低温胁迫下，黄瓜幼苗叶绿素和可溶性蛋白含量显著降低，可溶性糖、脯氨酸（Pro）、丙二醛（MDA）含量以及超氧化物歧化酶（SOD）、过氧化物酶（POD）活性显著增加（P<0.01）；外施SA显著提高了低温胁迫下幼苗叶片的叶绿素、可溶性蛋白、可溶性糖和Pro含量，SOD、POD活性也显著升高，使膜脂过氧化产物MDA含量显著降低（P<0.01）。由此可见，外施SA可以通过提高黄瓜幼苗可溶性蛋白、可溶性糖、Pro含量以及SOD、POD活性来维持细胞膜的稳定性，降低膜脂过氧化伤害程度，从而缓解了低温胁迫对幼苗生长的抑制，并以1.5 mmol/L外源SA缓解效果最好。
- Abstract:
- Using cucumber ‘Jinyan-2’ as the material，the effects of different concentrations of salicylic acid on the growth of cucumber seedling under 4℃ cold stress were studied by hydroponic culture.The results showed that under cold stress，the contents of chlorophyll and soluble proteins were significantly decreased than those of control.The contents of proline，soluble sugar，MDA and the activities of SOD and POD were significantly increased(P<0.01).Exogenous SA application on the root significantly increased the contents of chlorophyll，soluble proteins，soluble sugar and proline，while the activities of SOD and POD were significantly increased，the membrane lipid peroxidation products MDA content decreased significantly (P<0.01).Therefore，the application of SA could improve the conptents of cucumber seedling soluble protein,soluble sugar and Pro and the activities of SOD and POD to maintain the stability of cell membrane，decrease membrane lipid peroxidation damage degree，so as to alleviate the low temperature stress on seedling growth inhibition，and the alleviated effect was the best under the condition of 1.5 mmol/L exogenous SA.
参考文献/References:
[1]郑丽梅,司龙亭,韩贵超.低温处理对不同耐寒性黄瓜幼苗叶片超微结构的影响[J].西北农业学报,2009,18(4):276-279.
[2]周青,王纪忠,陈新红.持续低温对黄瓜幼苗形态和生理特性的影响[J].北方园艺,2010(16):1-3.
[3]刘伟,艾希珍,梁文娟,等.低温弱光下水杨酸对黄瓜幼苗光合作用及抗氧化酶活性的影响[J].应用生态学报,2009,20(2):441-445.
[4]孟雪娇,邸昆,丁国华.水杨酸在植物体内的生理作用研究进展[J].中国农学通报,2010,26(15):207-214.
[5]Kang G Z，Wang C H，Sun G C，et al.Salicylic acid changes activities of H2O2-metabolizing enzymes and increases the chilling tolerance of banana seedlings[J].Environmental and Experimental Botany,2003,50(1):9-15.
[6]常云霞,徐克东,陈璨,等.水杨酸对低温胁迫下大豆幼苗生长抑制的缓解作用[J].大豆科学,2012,31(6):927-931.
[7]张蕊,高志明,吕俊,等.外源水杨酸对水稻幼苗耐寒性的影响[J].江苏农业科学,2012,40(6):62-65.
[8]初敏,王秀峰,王淑芬,等.外源SA预处理对低温胁迫下萝卜幼苗的生理效应[J].西北农业学报,2012,21(2):142-145,183.
[9]王志斌,葛云侠,王建民,等.水杨酸和赤霉素复配剂对低温下玉米光合特性的影响[J].玉米科学,2010,18(6):43-45.
[10]刘萍,李明军.植物生理学实验技术[M].北京:科学出版社,2008.
[11]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[12]周建,杨立峰,郝峰鸽,等.低温胁迫对广玉兰幼苗光合及叶绿素荧光特性的影响[J].西北植物学报,2009,29(1):136-142.
[13]冀鹏飞,薛斌,刘志华,等.干旱胁迫下葡萄根系的生理生化变化与抗旱性的关系[J].北方园艺,2012(4):17-20.[14]唐仕云,杨丽涛,李杨瑞.低温胁迫下不同甘蔗品种（系）光合特性的变化及其与耐寒性的关系[J].广西植物,2012,32(5):679-685.
[15]孙富,杨丽涛,谢晓娜,等.低温胁迫对不同抗寒性甘蔗品种幼苗叶绿体生理代谢的影响[J].作物学报,2012,38(4):732-739.
[16]张良英,刘林,牛歆雨.UV-B辐射增强对油桃光合特性和抗氧化酶活性的影响[J].北方园艺,2012(11):27-29.[17]马迪,岳桦.大叶铁线莲对低温胁迫的响应[J].北方园艺,2010(6):112-114.
[18]朱月,赵雪梅,唐立红.低温对几种引种紫斑牡丹叶片可溶性糖含量的影响[J].北方园艺,2012(2):62-64.
[19]陈菲.低温胁迫对耧斗菜脯氨酸和可溶性蛋白含量的影响[J].北方园艺,2011(5):29-31.
相似文献/References:
[1]关志华,王忠红.三十四份樱桃番茄种质材料耐低温性鉴定[J].北方园艺,2014,38(13):24.
GUAN Zhi-hua,WANG Zhong-hong.Cold Tolerance Appraisal on Seed of 34 Cherry Tomato Germplasm[J].Northern Horticulture,2014,38(12):24.
[2]杨忠义,段国锋,马金虎,等.不同处理方法对苦参种子发芽的影响[J].北方园艺,2014,38(12):124.
YANG Zhong-yi,DUAN Guo-feng,MA Jin-hu,et al.Effect of Different Treatment Methods on Seed Germination of Sophora flavescens [J].Northern Horticulture,2014,38(12):124.
[3]付雪清,李红利,朱翠敏,等.水杨酸和亚硫酸氢钠降低小白菜硝酸盐累积的复合效应研究[J].北方园艺,2014,38(04):9.
FU Xue-qing,LI Hong-li,ZHU Cui-min,et al.The Compound Effect of SA and Sodium Bisulfite on Reducing Nitrate Accumulation in the Leaf of Non-heading Chinese Cabbage[J].Northern Horticulture,2014,38(12):9.
[4]王宁.植物抗寒生理性研究进展[J].北方园艺,2014,38(04):174.
WANG Ning.Research Progress on Cold Resistance Physiology of Plant[J].Northern Horticulture,2014,38(12):174.
[5]关志华,王忠红.十份加工番茄种质材料耐低温筛选研究[J].北方园艺,2014,38(02):34.
GUAN Zhi- hua,WANG Zhong- hong.Study on Low Temperature Resistant Screening of 10 Processing Tomato Germplasm Materials[J].Northern Horticulture,2014,38(12):34.
[6]高彩琴,范永,代红军,等.水杨酸对“赤霞珠”葡萄中白藜芦醇诱导合成的影响[J].北方园艺,2014,38(06):20.
GAO Cai-qin,FAN Yong,DAI Hong-jun,et al.Effect of SA on the Accumulation of Resveratrol in Carbernet-Sauvignon Grape[J].Northern Horticulture,2014,38(12):20.
[7]张 红.阿司匹林浸种对低温胁迫下甜瓜幼苗生理特性的影响[J].北方园艺,2013,37(18):1.
ZHANG Hong.Effects of Aspirin on Physiological Characteristics of Muskmelon Seedlings Under Chilling Stress[J].Northern Horticulture,2013,37(12):1.
[8]籍越,江涓.水杨酸对香石竹切花衰老过程中糖代谢的影响[J].北方园艺,2013,37(05):142.
JI YueJIANG Juan.Effects of Salicylic Acid on the Metabolism of Saccharide in Cut Flower of Dianthus caryophyllus Senescence[J].Northern Horticulture,2013,37(12):142.
[9]师秋菊,李群.植物耐受低温胁迫研究进展[J].北方园艺,2013,37(05):191.
SHI Qiu-ju,LI Qun.Research Progress of Plant Resistance to Low Temperature Stress[J].Northern Horticulture,2013,37(12):191.
[10]王永亮,王韡,戴斌,等.“星白”勋章菊耐寒性研究[J].北方园艺,2012,36(02):73.
WANG Yong-liang,WANG Wei,DAI Bin,et al.Study on Cold Hardiness of Gazania rigens L.‘White Star’[J].Northern Horticulture,2012,36(12):73.
[11]庄福金,陈可,李敏.施用水杨酸配合接种菌根改善黄瓜抗低温的效应[J].北方园艺,2017,41(20):7.[doi:10.11937/bfyy.20171005]
ZHUANG Fujin,CHEN Ke,LI Min.Effects of Application of Salicylic Acid With Inoculation of Arbuscular Mycorrhizal Fungi on Improving Cucumber Resistance to Chilling[J].Northern Horticulture,2017,41(12):7.[doi:10.11937/bfyy.20171005]
[12]陈明辉,程世平,佟伟霜,等.水杨酸对低温胁迫下铁皮石斛幼苗根系活力及抗氧化酶活性的影响[J].北方园艺,2017,41(22):83.[doi:10.11937/bfyy.20172380]
CHEN Minghui,CHENG Shiping,TONG Weishuang,et al.Effects of SA on Root Activity and Antioxidant Enzyme Activity ofDendrobium officinale Seedlings Under Low Temperature Stress[J].Northern Horticulture,2017,41(12):83.[doi:10.11937/bfyy.20172380]
[13]刘耀臣,陈可,于伟红,等.水杨酸和AM真菌增强黄瓜耐低温的效应[J].北方园艺,2020,44(10):10.[doi:10.11937/bfyy.20193391]
LIU Yaochen,CHEN Ke,YU Weihong,et al.Effects of Salicylic Acid and AM Fungi on Cucumber Tolerance to Low Temperature[J].Northern Horticulture,2020,44(12):10.[doi:10.11937/bfyy.20193391]
[14]刘紫烟,李丹,朱圆圆,等.外源SA对核桃一年生枝低温胁迫的生理影响[J].北方园艺,2022,(13):25.[doi:10.11937/bfyy.20214979]
LIU Ziyan,LI Dan,ZHU Yuanyuan,et al.Physiological Effects of Exogenous SA on Low Temperature Stress of Walnut Annual Branches[J].Northern Horticulture,2022,(12):25.[doi:10.11937/bfyy.20214979]
[15]马曙晓,席素亭,朱铮.外源水杨酸对低温胁迫月季苗生理指标的影响[J].北方园艺,2023,(07):70.[doi:10.11937/bfyy.20223709]
MA Shuxiao,XI Suting,ZHU Zheng.Effects of Exogenous Salicylic Acid on Physiological Indexes in Rose Seedling Under Low Temperature Stress[J].Northern Horticulture,2023,(12):70.[doi:10.11937/bfyy.20223709]
[16]刘羽琪,孙梦笛,冯国军,等.水杨酸对低温胁迫下菜豆幼苗生长及抗寒性的影响[J].北方园艺,2023,(24):1.[doi:10.11937/bfyy.20232619]
LIU Yuqi,SUN Mengdi,FENG Guojun,et al.Effects of Salicylic Acid on the Growth and Cold Resistance of Common Bean Seedlings Under Low Temperature Stress[J].Northern Horticulture,2023,(12):1.[doi:10.11937/bfyy.20232619]
备注/Memo
第一作者简介：常云霞（1978-），女，硕士，讲师，现主要从事植物抗性生理等研究工作。E-mail：changyx618@126.com.
责任作者：陈龙（1962-），男，本科，教授，现主要从事生物化学与分子生物学等研究工作。E-mail：chenlongzg@126.com.
基金项目：河南省教育厅自然科学研究计划资助项目（2011B180057）；周口师范学院生物化学与分子生物学重点学科建设资助项目。
收稿日期：2012-03-04